FastQCFastQC Report
Wed 25 May 2016
SRR1779715_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779715_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166405
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACAG200.006926802552.5284163
TCAACGA200.006926802552.5284163
CGTATTA200.006943295752.49684556
CTCGGTT608.367351E-1152.49684544
ACGGATG200.006943295752.49684569
AAGGCGT200.006943295752.49684539
TATCTCG506.161645E-848.99705541
CGGTGGT1800.048.6081946
TCGGTGT651.8371793E-1048.45862645
TCTCGGT1900.047.89185743
GTTAGTG305.866577E-446.66386432
ACGATTG305.866577E-446.66386424
TTGGTCG451.6777449E-646.66386448
GACGATT451.6777449E-646.66386423
ATCTCGT851.8189894E-1245.29139742
CGTATCA950.044.2078756
CGGTTGT404.5794266E-543.7473746
GTAGTGT404.5794266E-543.7473733
CCGACTA757.4942363E-1041.9974821
TCGGTGG1500.041.9974845
Produced by FastQC (version 0.11.5)