##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779715_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	166405
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.915753733361377	34.0	23.0	34.0	12.0	34.0
2	27.596370301373156	33.0	23.0	34.0	12.0	34.0
3	26.71387878969983	32.0	21.0	34.0	11.0	34.0
4	28.757447192091583	33.0	23.0	34.0	12.0	34.0
5	28.351077191190168	34.0	23.0	34.0	11.0	34.0
6	30.56129323037168	36.0	26.0	38.0	11.0	38.0
7	31.55343288963673	37.0	27.0	38.0	12.0	38.0
8	30.780661638772873	36.0	26.0	38.0	11.0	38.0
9	32.22319040894204	37.0	31.0	38.0	12.0	38.0
10-11	31.841738529491302	37.0	28.0	38.0	11.0	38.0
12-13	30.990895706258826	36.0	27.0	38.0	11.0	38.0
14-15	30.890580210931162	36.5	26.5	38.0	11.0	38.0
16-17	30.6135332471981	35.5	26.5	38.0	11.0	38.0
18-19	31.1891259277065	36.5	26.5	38.0	11.5	38.0
20-21	30.098680929058624	35.0	24.0	38.0	11.0	38.0
22-23	30.09060725338782	35.5	24.5	38.0	11.0	38.0
24-25	31.202557014512784	36.5	27.0	38.0	11.0	38.0
26-27	29.82446440912232	35.0	24.0	38.0	11.0	38.0
28-29	31.80309185421111	37.0	29.0	38.0	11.0	38.0
30-31	32.264955980889994	37.0	31.0	38.0	11.0	38.0
32-33	32.34979417685767	37.0	31.0	38.0	11.0	38.0
34-35	32.408999128631955	37.0	31.0	38.0	11.0	38.0
36-37	30.439139448934828	36.0	25.0	38.0	11.0	38.0
38-39	28.824103843033562	34.0	22.0	37.5	11.0	38.0
40-41	29.87765091193173	35.0	24.5	37.5	11.0	38.0
42-43	30.87544544935549	36.0	25.5	38.0	11.0	38.0
44-45	32.41760463928368	37.0	31.0	38.0	11.0	38.0
46-47	31.045614614945464	36.5	27.5	38.0	11.0	38.0
48-49	30.457699588353716	35.5	24.5	38.0	11.0	38.0
50-51	28.421060064300953	34.0	21.0	37.5	11.0	38.0
52-53	31.544583996875094	36.5	28.5	38.0	11.0	38.0
54-55	30.675784982422403	36.0	25.5	38.0	11.0	38.0
56-57	31.078951954568673	36.5	27.0	38.0	11.0	38.0
58-59	32.097500075117935	37.0	31.0	38.0	11.0	38.0
60-61	32.57883477058983	37.5	32.0	38.0	11.0	38.0
62-63	32.46375409392746	37.5	31.5	38.0	11.0	38.0
64-65	32.18459180914035	37.5	30.0	38.0	11.0	38.0
66-67	30.19024668729906	36.0	24.0	38.0	11.0	38.0
68-69	30.249130134310867	36.0	24.5	38.0	11.0	38.0
70-71	29.83102971665515	35.0	24.0	38.0	11.0	38.0
72-73	28.68658093206334	34.0	21.5	37.5	11.0	38.0
74-75	30.66216459841952	35.5	25.5	38.0	11.0	38.0
76	31.001177849223282	36.0	26.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	2.0
5	2.0
6	0.0
7	0.0
8	1.0
9	1.0
10	1.0
11	4.0
12	60.0
13	324.0
14	867.0
15	1513.0
16	2442.0
17	3769.0
18	5030.0
19	5312.0
20	4931.0
21	3889.0
22	3274.0
23	2805.0
24	2657.0
25	2750.0
26	2983.0
27	3415.0
28	4035.0
29	4887.0
30	5777.0
31	7267.0
32	9276.0
33	12371.0
34	16444.0
35	21500.0
36	24815.0
37	13999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.94291036927977	16.07944472822331	10.793545867011208	33.184099035485715
2	20.796250112676905	16.500105165109222	38.18995823442805	24.513686487785826
3	21.882286483497992	15.192543448474796	35.80322588399317	27.121944184034035
4	15.458162063411937	17.50679070214658	30.112857863993653	36.922189370447825
5	15.853489979267449	26.31291127069499	38.201376160572096	19.63222258946546
6	29.803791953366787	31.408311048345904	15.656981460893604	23.130915537393708
7	28.22210871067576	25.02088278597398	18.402091283314803	28.354917220035457
8	24.45747871080956	30.66304485002915	17.302988599691105	27.576487839470186
9	23.612663613745358	15.471940768518852	18.32834529261169	42.587050325124096
10-11	25.484757883982635	22.335071139890925	25.821902372331312	26.358268603795125
12-13	25.002403846153847	18.13311298076923	28.207031249999996	28.65745192307692
14-15	24.855694544187067	21.19494833248698	22.54560533171475	31.4037517916112
16-17	26.044875011643942	21.229840951227647	24.419530576247556	28.305753460880855
18-19	23.108959623549772	21.033495295874612	26.78451770319688	29.07302737737873
20-21	25.65377692707567	20.7802353987566	25.779979987800374	27.786007686367363
22-23	26.17332696300703	20.035277184315774	25.717187561036443	28.07420829164075
24-25	24.12214015709229	23.407292111130477	22.03318529558471	30.43738243619252
26-27	24.964993780010698	22.295506583573218	25.508266275638675	27.231233360777406
28-29	24.521481499287855	23.203564925269983	23.765767823123937	28.509185752318224
30-31	23.956646243806283	23.12340555954531	24.44943914758847	28.470509049059938
32-33	24.557086022668543	22.410425606077	24.72896308849866	28.303525282755803
34-35	24.192671163929745	22.674619510509473	24.564371459907754	28.56833786565303
36-37	25.00413169748162	20.326449174111232	29.173991039474988	25.495428088932158
38-39	25.007737542556484	18.867884841327793	32.35284396005926	23.77153365605647
40-41	24.405715230096607	21.88824952297961	24.376568157574482	29.329467089349297
42-43	21.874230013401522	22.463476343006867	25.91241533903449	29.74987830455712
44-45	22.196781211312636	20.413406411134748	28.375040565391412	29.014771812161204
46-47	23.928172213608338	20.012860731499178	28.74523732256397	27.31372973232851
48-49	24.209716579725473	20.539556239332676	29.017824947715088	26.232902233226763
50-51	26.009248881450254	19.275414141353437	30.304960681736915	24.410376295459393
52-53	20.68379047573259	22.56785018870646	28.142803913555614	28.605555422005335
54-55	23.018813496678113	21.30573659902702	27.404122081894748	28.27132782240011
56-57	23.098303760573327	21.632236061238903	26.477861746720954	28.79159843146682
58-59	20.652778153386638	22.765202393079182	27.517105227573897	29.06491422596028
60-61	20.81407717687218	21.96944656454143	27.361971670162323	29.85450458842407
62-63	21.14611441312043	22.864895400758428	26.871878699736172	29.11711148638497
64-65	21.349587437424503	22.98751795383386	26.149195608146687	29.513699000594958
66-67	20.027885369496172	24.939676854241853	22.338945464270367	32.69349231199161
68-69	20.65074957021435	24.734614875993316	22.63918442913646	31.975451124655873
70-71	20.735412103018994	25.112307049727605	22.08670254482516	32.065578302428236
72-73	19.974699215134798	25.6508491688602	21.59971874661955	32.774732869385446
74-75	21.978665865384617	25.046875	24.186298076923077	28.788161057692307
76	22.26843032025818	24.506451438427376	24.666911062098475	28.558207179215973
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	1.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	1.0
10	1.0
11	0.0
12	0.0
13	1.5
14	5.0
15	9.0
16	13.5
17	16.0
18	24.0
19	42.5
20	83.5
21	114.0
22	123.5
23	179.5
24	281.5
25	337.0
26	386.5
27	552.5
28	813.5
29	958.0
30	1122.5
31	1516.0
32	2008.5
33	2272.0
34	2493.5
35	2951.5
36	3545.5
37	3903.0
38	4233.5
39	4816.0
40	5400.0
41	5896.5
42	6061.0
43	6239.5
44	6508.0
45	6748.5
46	6899.0
47	6894.5
48	6931.5
49	6876.5
50	6780.0
51	6651.0
52	6473.5
53	6294.5
54	6164.0
55	6026.5
56	5831.5
57	5592.5
58	5411.0
59	5299.5
60	5029.0
61	4736.5
62	4603.0
63	4470.5
64	4120.5
65	3769.0
66	3474.0
67	3313.0
68	3263.5
69	3047.5
70	2749.0
71	2617.0
72	2508.5
73	2250.5
74	1921.0
75	1741.0
76	1576.5
77	1311.0
78	1028.5
79	847.0
80	746.5
81	535.5
82	363.5
83	302.0
84	243.0
85	154.5
86	101.5
87	78.0
88	57.5
89	31.0
90	20.0
91	11.5
92	8.0
93	6.0
94	3.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	6.00943481265587E-4
4	6.00943481265587E-4
5	0.0
6	0.0
7	0.0
8	0.0036056608875935222
9	0.004206604368859109
10-11	0.004507076109491902
12-13	0.003004717406327935
14-15	0.002704245665695141
16-17	0.005708963072023077
18-19	0.005108019590757489
20-21	0.0039061326282263152
22-23	0.005108019590757489
24-25	0.0036056608875935222
26-27	0.0036056608875935222
28-29	0.0036056608875935222
30-31	0.0039061326282263152
32-33	0.004206604368859109
34-35	0.004507076109491902
36-37	0.005108019590757489
38-39	0.005108019590757489
40-41	0.004507076109491902
42-43	0.0036056608875935222
44-45	0.004206604368859109
46-47	0.004206604368859109
48-49	0.005408491331390282
50-51	0.0039061326282263152
52-53	0.005408491331390282
54-55	0.005108019590757489
56-57	0.004507076109491902
58-59	0.005108019590757489
60-61	0.004807547850124696
62-63	0.004807547850124696
64-65	0.0036056608875935222
66-67	0.005708963072023077
68-69	0.02584056969442024
70-71	0.005108019590757489
72-73	0.004206604368859109
74-75	0.003004717406327935
76	0.004807547850124696
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	166405.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.50778109602896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.76156014928064	93.38243465397015
2	2.898976297462541	5.595475398361828
3	0.30179286924874704	0.8737608048540173
4	0.03465685678989358	0.133786253942219
5	0.003013827218202448	0.014542888871776788
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.00943481265587E-4	0.0
2	0.0	0.0	0.0	0.001201886962531174	0.0
3	0.0	0.0	0.0	0.001201886962531174	0.0
4	0.0	0.0	0.0	0.004807547850124696	0.0
5	0.0	0.0	0.0	0.005408491331390283	0.0
6	0.0	0.0	0.0	0.005408491331390283	0.0
7	0.0	0.0	0.0	0.00600943481265587	0.0
8	0.0	0.0	0.0	0.007211321775187044	0.0
9	0.0	0.0	0.0	0.015624530512905261	0.0
10	0.0	0.0	0.0	0.017427360956702023	0.0
11	0.0	0.0	0.0	0.01802830443796761	0.0
12	0.0	0.0	0.0	0.019230191400498784	0.0
13	0.0	0.0	0.0	0.01983113488176437	0.0
14	0.0	0.0	0.0	0.021033021844295546	0.0
15	0.0	0.0	0.0	0.02403773925062348	0.0
16	0.0	0.0	0.0	0.02584056969442024	0.0
17	0.0	0.0	0.0	0.028845287100748174	0.0
18	0.0	0.0	0.0	0.03004717406327935	0.0
19	0.0	0.0	0.0	0.034253778432138456	0.0
20	0.0	0.0	0.0	0.042666987169856674	0.0
21	0.0	0.0	0.0	0.044469817613653435	0.0
22	0.0	0.0	0.0	0.05348396983263724	0.0
23	0.0	0.0	0.0	0.06189717857035546	0.0
24	0.0	0.0	0.0	0.06490189597668339	0.0
25	0.0	0.0	0.0	0.07091133078933927	0.0
26	0.0	0.0	0.0	0.08353114389591659	0.0
27	0.0	0.0	0.0	0.13641417024728825	0.0
28	0.0	0.0	0.0	0.18268681830473843	0.0
29	0.0	0.0	0.0	0.20912833148042426	0.0
30	0.0	0.0	0.0	0.27403022745710764	0.0
31	0.0	0.0	0.0	0.2950632493014032	0.0
32	0.0	0.0	0.0	0.33292268862113517	0.0
33	0.0	0.0	0.0	0.3713830714221327	0.0
34	0.0	0.0	0.0	0.4362849673988161	0.0
35	0.0	0.0	0.0	0.6003425377843213	0.0
36	0.0	0.0	0.0	0.728944442775157	0.0
37	0.0	0.0	0.0	1.132177518704366	0.0
38	0.0	0.0	0.0	1.5155794597518104	0.0
39	0.0	0.0	0.0	1.945254048856705	0.0
40	0.0	0.0	0.0	2.423605059944112	0.0
41	0.0	0.0	0.0	2.9776749496709836	0.0
42	0.0	0.0	0.0	3.606862774556053	0.0
43	0.0	0.0	0.0	4.263093056098074	0.0
44	0.0	0.0	0.0	4.872449746101379	0.0
45	0.0	0.0	0.0	5.3009224482437425	0.0
46	0.0	0.0	0.0	5.838165920495177	0.0
47	0.0	0.0	0.0	6.44511883657342	0.0
48	0.0	0.0	0.0	6.822511342808209	0.0
49	0.0	0.0	0.0	7.1728613923860465	0.0
50	0.0	0.0	0.0	7.689672786274451	0.0
51	0.0	0.0	0.0	8.285207776208647	0.0
52	0.0	0.0	0.0	8.833268231122863	0.0
53	0.0	0.0	0.0	9.542381539016256	6.00943481265587E-4
54	0.0	0.0	0.0	10.22685616417776	6.00943481265587E-4
55	0.0	0.0	0.0	10.651122261951263	6.00943481265587E-4
56	0.0	0.0	0.0	11.11204591208197	6.00943481265587E-4
57	0.0	0.0	0.0	11.49725068357321	6.00943481265587E-4
58	0.0	0.0	0.0	11.830173372194345	6.00943481265587E-4
59	0.0	0.0	0.0	12.20936870887293	6.00943481265587E-4
60	0.0	0.0	0.0	12.432919683903728	6.00943481265587E-4
61	0.0	0.0	0.0	12.693128211291729	6.00943481265587E-4
62	0.0	0.0	0.0	12.88482918181545	6.00943481265587E-4
63	0.0	0.0	0.0	13.158859409272559	6.00943481265587E-4
64	0.0	0.0	0.0	13.43349058021093	6.00943481265587E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACAG	20	0.0069268025	52.52841	63
TCAACGA	20	0.0069268025	52.52841	63
CGTATTA	20	0.0069432957	52.496845	56
CTCGGTT	60	8.367351E-11	52.496845	44
ACGGATG	20	0.0069432957	52.496845	69
AAGGCGT	20	0.0069432957	52.496845	39
TATCTCG	50	6.161645E-8	48.997055	41
CGGTGGT	180	0.0	48.60819	46
TCGGTGT	65	1.8371793E-10	48.458626	45
TCTCGGT	190	0.0	47.891857	43
GTTAGTG	30	5.866577E-4	46.663864	32
ACGATTG	30	5.866577E-4	46.663864	24
TTGGTCG	45	1.6777449E-6	46.663864	48
GACGATT	45	1.6777449E-6	46.663864	23
ATCTCGT	85	1.8189894E-12	45.291397	42
CGTATCA	95	0.0	44.20787	56
CGGTTGT	40	4.5794266E-5	43.74737	46
GTAGTGT	40	4.5794266E-5	43.74737	33
CCGACTA	75	7.4942363E-10	41.99748	21
TCGGTGG	150	0.0	41.99748	45
>>END_MODULE