FastQCFastQC Report
Wed 25 May 2016
SRR1779715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779715_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166405
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2046312.297106457137707No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG24841.4927436074637181No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC14820.8905982392355998No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT8360.5023887503380308Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC6770.4068387368168024No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC5160.3100868363330429No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2990.1796821008984105No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG2210.1328085093596947No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT2120.12740001802830442No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1920.11538114840299271No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1920.11538114840299271No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1910.11478020492172712No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT1690.1015594483338842Illumina PCR Primer Index 9 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACA150.00222155370.070
ATTGACA150.00222155370.058
AAACGAC252.385079E-455.99999669
CAAACTT200.00694164752.49999624
CTTTGCG200.00694164752.49999626
CCCGCTA200.00694164752.49999627
TACAAGC200.00694164752.49999640
TATGCCG32200.051.5217443
CCGTCTT32050.051.43525747
GTCTTCT32050.051.43525749
TCGTATG32350.051.39103740
CGTCTTC32150.051.3841448
TGCTTGA32050.051.32605455
CGTATGC32400.051.31172641
TCTCGTA31650.051.31121438
ATGCCGT32200.051.30434844
CTCGTAT32100.051.24610539
ATCTCGT31650.051.2006337
GCCGTCT32200.051.08695646
CTGCTTG32350.051.0664654
Produced by FastQC (version 0.11.5)