FastQCFastQC Report
Wed 25 May 2016
SRR1779714_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779714_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194465
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA207.914963E-569.9948636
TAGATCT1600.059.0581639
CCGTATC1200.052.49614355
TAGTGTA200.00694541252.49614334
TTTAGAT900.050.55183837
GTAGATC1250.050.39629738
CGTATCA1100.047.7237756
GTATCAT1100.047.7237757
TATCACG305.8694166E-446.6632411
ATCTCGG2150.043.9502642
CGTATCC404.582649E-543.74678456
GTTTAGA802.910383E-1143.74678436
GACGAAA404.582649E-543.74678423
TTAGATC901.8189894E-1242.7746338
AGATCTC2050.042.67978740
CTCGGTG2550.042.54589544
TCGGTGG1900.042.36530745
TCTCGGT2650.042.26104443
GATCTCG2250.041.99691841
CGACGAT701.9153958E-839.99706322