FastQCFastQC Report
Wed 25 May 2016
SRR1779714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779714_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194465
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT1972710.144241894428303No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG24511.26038104543234No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC14340.7374077597511121No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT8670.445838582778392RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC6650.3419638495359062No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC5570.28642686344586427RNA PCR Primer, Index 32 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC3700.1902656004936621No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT2260.11621628570693955No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC2180.11210243488545496RNA PCR Primer, Index 32 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG2130.10953127812202709No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGT150.002222118270.058
TTGCGTC150.002222118270.04
CTAATCG150.002222118270.017
GTCTAGG308.354047E-658.3333321
ATTCGAT200.00694339852.532
CGGTGTT200.00694339852.537
CGTAATA200.00694339852.562
ACGTAAT200.00694339852.561
ATGCCGT27850.051.0233444
CGTATGC27950.050.9660141
TCGTATG28000.050.87540
TATGCCG27950.050.84078643
CTCGTAT27850.050.7719939
GCCGTCT28000.050.7546
TCTTCTG28150.050.7282450
GTATGCC28100.050.6939542
CTGCTTG27750.050.57657654
GCTTGAA27550.050.56261456
TGCTTGA27750.050.45044755
CCGTCTT28100.050.44484347