FastQCFastQC Report
Wed 25 May 2016
SRR1779713_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779713_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149971
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACT207.908804E-569.9953238
GTATCAC308.348055E-658.3294457
CTACGAA452.6839189E-854.44081522
TGCCTAC900.054.44081519
AGTCCAT200.006942144652.49649421
AATTTGG200.006942144652.49649442
CGTACCC200.006942144652.49649456
CGTGTAT200.006942144652.49649435
TAGATCT1550.051.9320239
CGTATCA1150.048.69240656
GTATCAT1150.048.69240657
TTTAGTC305.8649E-446.66355518
TCGGAGT305.8649E-446.66355531
AGGTGGT751.4551915E-1146.6635546
AGATCTC1750.045.99693340
TATCATT1450.045.8590158
TCGGTGG2100.044.99699845
TCTCGGT2350.044.67786843
CATTAAA1100.044.54248461
ATCATTA1100.044.54248459
Produced by FastQC (version 0.11.5)