##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779713_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	149971
Sequences flagged as poor quality	0
Sequence length	76
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.289382613972034	33.0	23.0	34.0	12.0	34.0
2	27.13270565642691	32.0	23.0	34.0	12.0	34.0
3	26.195131058671343	32.0	21.0	34.0	11.0	34.0
4	28.105660427682686	33.0	23.0	34.0	12.0	34.0
5	27.783271432476944	33.0	23.0	34.0	11.0	34.0
6	29.781637783304774	35.0	23.0	38.0	11.0	38.0
7	30.895479792759932	36.0	26.0	38.0	12.0	38.0
8	30.081055670763014	36.0	24.0	38.0	11.0	38.0
9	31.59493502077068	37.0	27.0	38.0	12.0	38.0
10-11	31.11251508625001	37.0	26.5	38.0	11.0	38.0
12-13	30.449363543618432	35.5	25.0	38.0	11.0	38.0
14-15	30.179984797060765	35.0	24.5	38.0	11.0	38.0
16-17	29.90471824552747	35.5	24.0	38.0	11.0	38.0
18-19	30.463119536443713	36.0	25.0	38.0	11.0	38.0
20-21	29.35331164025045	35.0	23.5	38.0	11.0	38.0
22-23	29.382657313747323	34.5	23.5	37.5	11.0	38.0
24-25	30.38816504524208	35.5	24.5	38.0	11.0	38.0
26-27	29.06907335418181	34.0	22.0	38.0	11.0	38.0
28-29	30.95708503644038	36.5	26.0	38.0	11.0	38.0
30-31	31.41198631735469	37.0	26.5	38.0	11.0	38.0
32-33	31.55796453981103	37.0	27.0	38.0	11.0	38.0
34-35	31.59609857905862	37.0	27.0	38.0	11.0	38.0
36-37	29.622973774929818	35.0	23.5	38.0	11.0	38.0
38-39	28.148075294556946	33.5	21.0	37.0	11.0	38.0
40-41	29.076851524628097	34.0	22.0	37.5	11.0	38.0
42-43	30.049826299751288	35.0	24.0	38.0	11.0	38.0
44-45	31.600459422154948	37.0	27.0	38.0	11.0	38.0
46-47	30.267451707330082	35.5	24.0	38.0	11.0	38.0
48-49	29.598752425468923	35.0	23.5	38.0	11.0	38.0
50-51	27.63170212907829	32.5	21.0	37.0	11.0	38.0
52-53	30.675130525234877	36.0	25.0	38.0	11.0	38.0
54-55	29.83171746537664	35.5	23.5	38.0	11.0	38.0
56-57	30.25206206533263	35.5	24.5	38.0	11.0	38.0
58-59	31.250448420027872	37.0	26.5	38.0	11.0	38.0
60-61	31.759123430529904	37.0	27.0	38.0	11.0	38.0
62-63	31.66559201445613	37.0	27.5	38.0	11.0	38.0
64-65	31.368277867054296	37.0	26.5	38.0	11.0	38.0
66-67	29.45752178754559	34.5	22.5	38.0	11.0	38.0
68-69	29.46732034860073	34.5	23.0	38.0	11.0	38.0
70-71	29.075334564682507	34.0	22.0	38.0	11.0	38.0
72-73	27.955698101632983	33.0	21.0	37.0	11.0	38.0
74-75	29.767104973628236	35.0	23.5	37.5	11.0	38.0
76	30.03149275526602	36.0	24.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	1.0
5	0.0
6	1.0
7	1.0
8	1.0
9	1.0
10	1.0
11	10.0
12	58.0
13	369.0
14	1004.0
15	1844.0
16	2846.0
17	4481.0
18	5551.0
19	5812.0
20	5234.0
21	4006.0
22	3266.0
23	2764.0
24	2551.0
25	2434.0
26	2831.0
27	3093.0
28	3629.0
29	4409.0
30	5251.0
31	6441.0
32	8265.0
33	10761.0
34	14337.0
35	18346.0
36	19847.0
37	10520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.49696941408673	16.519193710784087	12.121676857525788	31.8621600176034
2	20.61731934840736	15.713704649565582	39.088890518833644	24.580085483193418
3	21.334933653397346	15.105021004200841	36.59065146362606	26.969393878775755
4	16.50985857265738	18.14750851831354	26.9405418380887	38.40209107094038
5	17.0399610591381	25.616285815257616	36.992485213808	20.35126791179628
6	30.785951950710473	29.425022170953053	15.397643544418587	24.391382333917893
7	28.314140733875213	22.420334598022286	17.42003454001107	31.84549012809143
8	23.843563498149567	30.388423965591972	17.306038075550962	28.461974460707502
9	22.364551728736707	13.951255292901676	18.274930817190675	45.409262161170936
10-11	28.046438431069205	20.52186553926995	23.87671543457676	27.55498059508409
12-13	25.386008021898515	15.801072920591205	27.20843933064159	31.604479726868696
14-15	26.99638914543109	18.87079942253	20.528525084269944	33.604286347768976
16-17	28.52146253309238	18.716115738301802	22.353776698964385	30.408645029641434
18-19	25.052513653549923	17.959002674028582	24.683084268576497	32.305399403844994
20-21	28.03730216151156	17.97558755572152	23.514565113743586	30.472545169023334
22-23	28.6803612927318	17.165072368574602	23.381334542532585	30.773231796161017
24-25	25.968492123030757	20.816870884387765	19.265483037426023	33.94915395515545
26-27	27.3532314422128	20.095822997519406	23.0975434104185	29.453402149849296
28-29	26.90770148935248	20.41416468564227	21.519426264215866	31.158707560789388
30-31	26.117703543909602	20.111161864878653	22.218702800385433	31.55243179082631
32-33	26.69431765517839	19.63964805003851	22.437643575646234	31.22839071913686
34-35	26.43000986903524	19.7820810327812	22.07563148488971	31.712277613293853
36-37	27.838904781909964	16.676335847320303	27.35111128893512	28.13364808183461
38-39	26.930425011919805	15.89985362812207	31.464619016340972	25.705102343617153
40-41	26.634680465856007	20.45305263719446	22.121825414025693	30.790441482923836
42-43	23.50681825759344	20.717167339045776	24.17964191644717	31.596372486913616
44-45	23.151787946986747	18.326581645411352	26.684671167791947	31.836959239809953
46-47	26.422781847340815	17.425805956862263	26.51447204782561	29.636940147971313
48-49	26.4970658842358	17.937116564417177	26.97919445185383	28.586623099493195
50-51	29.038022965818005	16.794587962283778	28.09845160774063	26.06893746415759
52-53	22.446836177406126	19.772140756596716	26.72061402631351	31.060409039683652
54-55	25.364684229303442	18.703507970538517	25.218977250372603	30.71283054978544
56-57	25.285486134959505	19.283025536213813	24.534303347147805	30.897184981678876
58-59	22.625366764470524	20.685516137636704	25.510136036276343	31.17898106161643
60-61	22.917805844147185	19.429255411370878	25.13936864005548	32.51357010442646
62-63	22.462765443130404	20.78833567282269	25.04676200224724	31.702136881799664
64-65	22.899572229242448	21.367866180770655	24.454617290207285	31.277944299779612
66-67	21.79155038527069	24.71433953834202	20.05308099853627	33.44102907785102
68-69	21.783254681585486	24.94688922610015	20.52960696359786	32.740249128716506
70-71	22.121639509334187	25.251151144142252	19.948052640879705	32.679156705643855
72-73	21.196915187666086	25.920492396331035	19.441786337068763	33.44080607893412
74-75	23.14529868934774	23.597408702784346	22.783210581241438	30.47408202662648
76	23.831529531011398	22.443835397203273	22.97397323304059	30.75066183874474
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	1.5
6	1.0
7	0.5
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	0.5
14	0.0
15	1.0
16	4.0
17	6.0
18	6.5
19	12.5
20	25.0
21	32.0
22	47.5
23	79.0
24	114.5
25	134.0
26	198.0
27	294.0
28	425.0
29	524.0
30	625.0
31	812.5
32	1001.0
33	1103.0
34	1273.5
35	1623.0
36	2003.5
37	2205.0
38	2424.0
39	2860.0
40	3305.5
41	3690.5
42	3847.0
43	4003.5
44	4294.5
45	4536.0
46	4643.0
47	4641.0
48	4693.5
49	4701.5
50	4655.0
51	4676.0
52	4828.0
53	5042.5
54	5126.0
55	5323.0
56	5662.0
57	5926.0
58	6048.0
59	6174.5
60	6361.5
61	6530.0
62	6638.0
63	6488.5
64	6220.0
65	5921.0
66	5505.0
67	5269.0
68	5017.5
69	4465.0
70	3863.5
71	3563.0
72	3274.0
73	2717.0
74	2203.5
75	1958.0
76	1774.0
77	1385.0
78	1018.0
79	856.0
80	738.5
81	499.5
82	299.5
83	221.0
84	168.0
85	91.0
86	50.0
87	33.0
88	24.0
89	11.0
90	4.0
91	1.5
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	6.667955804788926E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.004000773482873355
9	0.004000773482873355
10-11	0.006001160224310033
12-13	0.004334171273112802
14-15	0.004334171273112802
16-17	0.00800154696574671
18-19	0.006667955804788926
20-21	0.004334171273112802
22-23	0.006334558014549479
24-25	0.005667762434070587
26-27	0.004667569063352248
28-29	0.004334171273112802
30-31	0.006334558014549479
32-33	0.004334171273112802
34-35	0.004667569063352248
36-37	0.006667955804788926
38-39	0.007001353595028373
40-41	0.007001353595028373
42-43	0.004000773482873355
44-45	0.005667762434070587
46-47	0.006334558014549479
48-49	0.007334751385267818
50-51	0.006001160224310033
52-53	0.006667955804788926
54-55	0.008334944755986158
56-57	0.005000966853591694
58-59	0.007334751385267818
60-61	0.006001160224310033
62-63	0.006334558014549479
64-65	0.005000966853591694
66-67	0.007668149175507265
68-69	0.03233958565322629
70-71	0.007668149175507265
72-73	0.007001353595028373
74-75	0.005000966853591694
76	0.006667955804788926
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	149971.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.87716340281305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.22711384409133	94.19086995057943
2	2.4016096408458956	4.65322259211998
3	0.31491841198713805	0.9152520736989711
4	0.042356722393898996	0.16413596466245375
5	0.010009445817357956	0.048484335900989516
6	0.001997044403300106	0.011608079818870659
7	0.0	0.0
8	9.974862721079487E-4	0.007730691246005167
9	9.974041889569613E-4	0.00869631197330104
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007334751385267818	0.0
2	0.0	0.0	0.0	0.008001546965746712	0.0
3	0.0	0.0	0.0	0.008668342546225604	0.0
4	0.0	0.0	0.0	0.012669116029098959	0.0
5	0.0	0.0	0.0	0.013335911609577852	0.0
6	0.0	0.0	0.0	0.013335911609577852	0.0
7	0.0	0.0	0.0	0.014002707190056744	0.0
8	0.0	0.0	0.0	0.014002707190056744	0.0
9	0.0	0.0	0.0	0.014669502770535636	0.0
10	0.0	0.0	0.0	0.016003093931493424	0.0
11	0.0	0.0	0.0	0.016669889511972315	0.0
12	0.0	0.0	0.0	0.017336685092451207	6.667955804788926E-4
13	0.0	0.0	0.0	0.017336685092451207	6.667955804788926E-4
14	0.0	0.0	0.0	0.017336685092451207	6.667955804788926E-4
15	0.0	0.0	0.0	0.017336685092451207	6.667955804788926E-4
16	0.0	0.0	0.0	0.019337071833887887	6.667955804788926E-4
17	0.0	0.0	0.0	0.02067066299484567	6.667955804788926E-4
18	0.0	0.0	0.0	0.022671049736282346	6.667955804788926E-4
19	0.0	0.0	0.0	0.024671436477719026	6.667955804788926E-4
20	0.0	0.0	0.0	0.026671823219155705	6.667955804788926E-4
21	0.0	0.0	0.0	0.03267298344346574	6.667955804788926E-4
22	0.0	0.0	0.0	0.0446753038920858	6.667955804788926E-4
23	0.0	0.0	0.0	0.04600889505304359	6.667955804788926E-4
24	0.0	0.0	0.0013335911609577852	0.05334364643831141	6.667955804788926E-4
25	0.0	0.0	0.0013335911609577852	0.058678011082142545	6.667955804788926E-4
26	0.0	0.0	0.0013335911609577852	0.06134519340405812	6.667955804788926E-4
27	0.0	0.0	0.0013335911609577852	0.10668729287662282	6.667955804788926E-4
28	0.0	0.0	0.0013335911609577852	0.1546965746711031	6.667955804788926E-4
29	0.0	0.0	0.0013335911609577852	0.19603790066079443	6.667955804788926E-4
30	0.0	0.0	0.0013335911609577852	0.24471397803575357	6.667955804788926E-4
31	0.0	0.0	0.0013335911609577852	0.26405104986964145	6.667955804788926E-4
32	0.0	0.0	0.0013335911609577852	0.3260630388541785	6.667955804788926E-4
33	0.0	0.0	0.0013335911609577852	0.3980769615458989	6.667955804788926E-4
34	0.0	0.0	0.0013335911609577852	0.5167665748711417	6.667955804788926E-4
35	0.0	0.0	0.0013335911609577852	0.7528122103606697	6.667955804788926E-4
36	0.0	0.0	0.0013335911609577852	0.9448493375385908	6.667955804788926E-4
37	0.0	0.0	0.0013335911609577852	1.4002707190056745	6.667955804788926E-4
38	0.0	0.0	0.0013335911609577852	1.8290202772536024	6.667955804788926E-4
39	0.0	0.0	0.0013335911609577852	2.2984443659107425	6.667955804788926E-4
40	0.0	0.0	0.0013335911609577852	2.7785371838555455	6.667955804788926E-4
41	0.0	0.0	0.0013335911609577852	3.244627294610291	6.667955804788926E-4
42	0.0	0.0	0.0013335911609577852	3.7460575711304185	6.667955804788926E-4
43	0.0	0.0	0.0013335911609577852	4.248154643231024	6.667955804788926E-4
44	0.0	0.0	0.0013335911609577852	4.758920057877856	6.667955804788926E-4
45	0.0	0.0	0.0013335911609577852	5.15432983710184	6.667955804788926E-4
46	0.0	0.0	0.0013335911609577852	5.649758953397657	6.667955804788926E-4
47	0.0	0.0	0.002000386741436678	6.16585873268832	6.667955804788926E-4
48	0.0	0.0	0.002000386741436678	6.535263484273626	6.667955804788926E-4
49	0.0	0.0	0.002000386741436678	6.885331164025045	6.667955804788926E-4
50	0.0	0.0	0.002000386741436678	7.375425915677031	6.667955804788926E-4
51	0.0	0.0	0.002000386741436678	7.874855805455722	6.667955804788926E-4
52	0.0	0.0	0.002000386741436678	8.288269065352635	0.0013335911609577852
53	0.0	0.0	0.002000386741436678	8.751691993785466	0.0013335911609577852
54	0.0	0.0	0.002000386741436678	9.241786745437452	0.0013335911609577852
55	0.0	0.0	0.002000386741436678	9.58518646938408	0.0013335911609577852
56	0.0	0.0	0.002000386741436678	9.964593154676571	0.0013335911609577852
57	0.0	0.0	0.002000386741436678	10.298657740496497	0.0013335911609577852
58	0.0	0.0	0.002000386741436678	10.605383707516786	0.0013335911609577852
59	0.0	0.0	0.002000386741436678	10.964119729814431	0.0013335911609577852
60	0.0	0.0	0.002000386741436678	11.1641584039581	0.0013335911609577852
61	0.0	0.0	0.002000386741436678	11.384200945516133	0.0013335911609577852
62	0.0	0.0	0.002000386741436678	11.520894039514307	0.0013335911609577852
63	0.0	0.0	0.002000386741436678	11.727600669462763	0.0013335911609577852
64	0.0	0.0	0.002000386741436678	11.934974094991698	0.0013335911609577852
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACACT	20	7.908804E-5	69.99532	38
GTATCAC	30	8.348055E-6	58.32944	57
CTACGAA	45	2.6839189E-8	54.440815	22
TGCCTAC	90	0.0	54.440815	19
AGTCCAT	20	0.0069421446	52.496494	21
AATTTGG	20	0.0069421446	52.496494	42
CGTACCC	20	0.0069421446	52.496494	56
CGTGTAT	20	0.0069421446	52.496494	35
TAGATCT	155	0.0	51.93202	39
CGTATCA	115	0.0	48.692406	56
GTATCAT	115	0.0	48.692406	57
TTTAGTC	30	5.8649E-4	46.663555	18
TCGGAGT	30	5.8649E-4	46.663555	31
AGGTGGT	75	1.4551915E-11	46.66355	46
AGATCTC	175	0.0	45.996933	40
TATCATT	145	0.0	45.85901	58
TCGGTGG	210	0.0	44.996998	45
TCTCGGT	235	0.0	44.677868	43
CATTAAA	110	0.0	44.542484	61
ATCATTA	110	0.0	44.542484	59
>>END_MODULE