FastQCFastQC Report
Wed 25 May 2016
SRR1779713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779713_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149971
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2332415.552340119089692No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG27001.80034806729301No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC17451.1635582879356676No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT10240.682798674410386RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC7600.5067646411639584No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC6050.40341132618973No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3480.23204486200665464No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG2600.17336685092451207No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2490.16603209953924425No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC2360.15736375699301866No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG2160.1440278453834408No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT1870.12469077354955291No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT1650.11002127077901727No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCG150.002221122870.000016
TCTTTAT1100.057.2727241
CCCCTTG200.00694031352.519
AAGACAG200.00694031352.554
TATAGGG200.00694031352.55
GCTAATC200.00694031352.533
TCTATAG200.00694031352.53
CTATACA1150.051.739131
GTATGCC33800.048.1508942
CGTATGC34050.048.10572441
TCGTATG34050.048.10572440
CTCGTAT33750.048.01481639
TCTCGTA33050.047.97276738
TTGAAAA34000.047.9705958
TCTTCTG33650.047.94948250
CTTGAAA34100.047.93255257
AATCTCG32550.047.8494636
CCGTCTT33650.047.8454747
TGCTTGA34100.047.82991455
GTCTTCT33850.047.7695749
Produced by FastQC (version 0.11.5)