##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779713_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	149971
Sequences flagged as poor quality	0
Sequence length	76
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.59180774949824	34.0	34.0	34.0	34.0	34.0
2	33.62200692133813	34.0	34.0	34.0	34.0	34.0
3	33.62194690973588	34.0	34.0	34.0	34.0	34.0
4	33.6673623567223	34.0	34.0	34.0	34.0	34.0
5	33.67315681031666	34.0	34.0	34.0	34.0	34.0
6	37.26688493108668	38.0	38.0	38.0	36.0	38.0
7	37.266698228324145	38.0	38.0	38.0	36.0	38.0
8	37.13383254095792	38.0	38.0	38.0	36.0	38.0
9	37.14386781444413	38.0	38.0	38.0	36.0	38.0
10-11	37.06093511412206	38.0	38.0	38.0	35.5	38.0
12-13	37.03405991825086	38.0	38.0	38.0	35.5	38.0
14-15	37.058277933733855	38.0	38.0	38.0	35.5	38.0
16-17	36.9901214234752	38.0	38.0	38.0	35.0	38.0
18-19	36.983833541151284	38.0	38.0	38.0	35.0	38.0
20-21	37.035333497809575	38.0	38.0	38.0	35.0	38.0
22-23	36.976715498329675	38.0	38.0	38.0	35.0	38.0
24-25	36.944439258256594	38.0	38.0	38.0	35.0	38.0
26-27	36.80466556867661	38.0	37.5	38.0	34.5	38.0
28-29	36.6931173360183	38.0	37.0	38.0	34.0	38.0
30-31	36.521767541724735	38.0	37.0	38.0	34.0	38.0
32-33	36.44217882123877	38.0	37.0	38.0	34.0	38.0
34-35	36.394606290549504	38.0	37.0	38.0	34.0	38.0
36-37	36.511165491995115	38.0	37.0	38.0	34.0	38.0
38-39	36.204072787405565	38.0	37.0	38.0	34.0	38.0
40-41	36.57267071633849	38.0	37.0	38.0	34.0	38.0
42-43	36.747034426655816	38.0	37.0	38.0	34.0	38.0
44-45	36.7095538470771	38.0	37.0	38.0	34.0	38.0
46-47	36.73999306532596	38.0	37.0	38.0	34.0	38.0
48-49	36.71646851724667	38.0	37.0	38.0	34.0	38.0
50-51	36.630371871895235	38.0	37.0	38.0	34.0	38.0
52-53	36.6161191163625	38.0	37.0	38.0	34.0	38.0
54-55	36.61588573790933	38.0	37.0	38.0	34.0	38.0
56-57	36.625117522721055	38.0	37.0	38.0	34.0	38.0
58-59	36.523534550013004	38.0	37.0	38.0	34.0	38.0
60-61	36.58006881330391	38.0	37.0	38.0	34.0	38.0
62-63	36.5757479779424	38.0	37.0	38.0	34.0	38.0
64-65	36.62140347133779	38.0	37.0	38.0	34.0	38.0
66-67	36.53221622847084	38.0	37.0	38.0	34.0	38.0
68-69	36.31588440431817	38.0	37.0	38.0	34.0	38.0
70-71	34.87514586153323	38.0	35.5	38.0	27.0	38.0
72-73	32.58068559921585	38.0	31.0	38.0	11.0	38.0
74-75	32.29339672336651	38.0	29.5	38.0	11.0	38.0
76	31.84068253195618	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	12.0
22	15.0
23	36.0
24	62.0
25	141.0
26	244.0
27	424.0
28	745.0
29	1195.0
30	1876.0
31	2806.0
32	4291.0
33	6437.0
34	10620.0
35	21226.0
36	26291.0
37	73546.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.618219522441	13.687312880490229	10.504697574864474	40.18977002220429
2	19.111028132105538	14.195411112815146	42.16348494042181	24.5300758146575
3	19.341739402951237	14.819531776143387	38.78349814297431	27.055230677931068
4	14.96889398617066	27.742696921404804	26.17039294263558	31.118016149788957
5	15.989758019883842	26.285748578058428	41.00526101712998	16.71923238492775
6	29.997132779003945	40.104420187902996	13.63196884731048	16.266478185782585
7	26.454447859919583	22.004920951383937	14.628161444545945	36.912469744150535
8	23.13847343819805	41.41200632122211	13.72732061531896	21.722199625260885
9	20.574644431256708	13.376586139987065	13.953364317101306	52.09540511165493
10-11	26.014696174593755	25.578945262750796	24.548746090910907	23.857612471744538
12-13	24.266024764787858	13.805335698234991	26.629481699795292	35.29915783718185
14-15	23.451867361023133	13.838342079468696	15.675697301478287	47.034093258029884
16-17	34.09959258790033	24.382047195791188	14.4147868587927	27.103573357515785
18-19	34.58268598595728	17.505050976522128	14.706509925252215	33.20575311226837
20-21	24.487067499716613	15.730041141287316	14.84153603029919	44.94135532869688
22-23	24.649432223563224	25.093518080162163	14.684839068886651	35.57221062738796
24-25	34.16393836141654	25.081515759713547	14.540144427922733	26.214401450947182
26-27	33.94289562648779	26.661487887658282	15.54067119643131	23.85494528942262
28-29	24.453060925112187	27.009221782878022	15.635022771069075	32.90269452094072
30-31	23.848610731408073	26.19273059458162	25.200538770829027	24.75811990318128
32-33	31.879163304905617	27.64301098212321	17.162984843736456	23.314840869234718
34-35	25.50493095331764	16.956278213787996	34.5286755439385	23.010115288955866
36-37	23.069460095618485	33.149408885717904	19.613791999786624	24.167339018876984
38-39	22.69438758159911	18.02115075581279	26.4317768101833	32.8526848524048
40-41	22.86875462589434	14.889211914303432	27.878056424242022	34.363977035560204
42-43	32.36492388528448	14.636162991511693	27.00655460055611	25.992358522647713
44-45	22.654379846770375	24.361376532796342	29.026611811616913	23.95763180881637
46-47	31.14835534870075	15.87040161097812	20.4612891825753	32.51995385774583
48-49	30.68493242026792	14.90254782591301	18.646271612511754	35.76624814130732
50-51	22.093938161377867	15.523667909129099	28.49784291629715	33.88455101319588
52-53	19.946522994445594	15.759713544618625	38.06369231384734	26.230071147088434
54-55	20.124557414433458	15.357969207380092	30.380206839989064	34.13726653819739
56-57	29.386348027285276	15.559008074894479	20.90037407232065	34.1542698254996
58-59	21.40980589580652	16.378499843303036	35.96828720219242	26.243407058698015
60-61	29.518373552220094	25.927012555760783	19.823832607637478	24.73078128438165
62-63	20.3142607570797	37.91666388835175	16.67955804788926	25.089517306679294
64-65	19.61212501083543	39.526308419627796	15.942082135879604	24.919484433657175
66-67	20.331930839962393	39.453961099145836	15.53400324062652	24.68010482026525
68-69	19.81516426509125	40.11142154149802	15.574344373245493	24.499069820165232
70-71	19.903514679504706	38.48477372291976	16.158790699535245	25.452920898040286
72-73	20.316261143821137	33.90955584746384	17.952137413233224	27.822045595481793
74-75	20.64065719372412	31.57377092904628	19.45909542511552	28.326476452114075
76	20.539304265491328	31.956844990031406	19.460429016276482	28.043421728200784
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.5
16	5.5
17	9.0
18	11.5
19	18.0
20	29.0
21	36.0
22	45.0
23	74.5
24	109.0
25	123.0
26	134.0
27	227.0
28	324.5
29	340.0
30	396.0
31	501.0
32	666.0
33	782.0
34	859.0
35	1215.5
36	6138.5
37	10782.0
38	11395.0
39	10713.0
40	7827.5
41	5138.0
42	4039.0
43	3353.5
44	2558.0
45	2532.5
46	2617.0
47	2516.0
48	2395.0
49	2386.0
50	2397.0
51	2481.0
52	2694.5
53	3031.0
54	3238.0
55	3435.5
56	3826.0
57	4277.0
58	4535.0
59	4725.0
60	5122.5
61	5461.0
62	5592.0
63	5599.0
64	5480.5
65	5395.5
66	5360.5
67	5285.0
68	4970.5
69	4436.0
70	4008.5
71	3801.0
72	3605.5
73	3171.0
74	2708.5
75	2485.0
76	2254.0
77	1780.0
78	1369.5
79	1202.0
80	1087.0
81	824.0
82	541.0
83	406.0
84	346.5
85	204.0
86	103.5
87	86.0
88	61.0
89	25.0
90	8.5
91	3.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	149971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.66958278600529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.194149268658585	22.697721559501506
2	25.959494936867106	22.15361636583073
3	12.31091386423303	15.759046749038147
4	5.136579572446555	8.767028292136478
5	1.908051006375797	4.070787018823639
6	0.7829103637954745	2.0043875149195514
7	0.31410176272034007	0.938181381733802
8	0.14220527565945743	0.48542718258863377
9	0.05156894611826478	0.1980382874022311
>10	0.16564570571321416	1.18022817744764
>50	0.012501562695336917	0.4387514919551114
>100	0.012501562695336917	1.197564862540091
>500	0.0031253906738342293	0.9101759673536883
>1k	0.004688086010751344	3.6467050296390635
>5k	0.0	0.0
>10k+	0.0015626953369171147	15.552340119089692
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT	23324	15.552340119089692	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	2700	1.80034806729301	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	1745	1.1635582879356676	No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT	1024	0.682798674410386	RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	760	0.5067646411639584	No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	605	0.40341132618973	No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	348	0.23204486200665464	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	260	0.17336685092451207	No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	249	0.16603209953924425	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	236	0.15736375699301866	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	216	0.1440278453834408	No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT	187	0.12469077354955291	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT	165	0.11002127077901727	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010668729287662281	0.0
2	0.0	0.0	0.0	0.012002320448620067	0.0
3	0.0	0.0	0.0	0.014002707190056744	0.0
4	0.0	0.0	0.0	0.02067066299484567	0.0
5	0.0	0.0	0.0	0.024004640897240134	0.0
6	0.0	0.0	0.0	0.024004640897240134	0.0
7	0.0	0.0	0.0	0.024671436477719026	0.0
8	0.0	0.0	0.0	0.02600502763867681	0.0
9	0.0	0.0	0.0	0.02800541438011349	0.0
10	0.0	0.0	0.0	0.029339005541071272	0.0
11	0.0	0.0	0.0	0.030005801121550168	0.0
12	0.0	0.0	0.0	0.03067259670202906	0.0
13	0.0	0.0	0.0	0.03067259670202906	0.0
14	0.0	0.0	0.0	0.03067259670202906	0.0
15	0.0	0.0	0.0	0.03133939228250795	0.0
16	0.0	0.0	0.0	0.03400657460442352	0.0
17	0.0	0.0	0.0	0.034673370184902415	0.0
18	0.0	0.0	0.0	0.038007348087296874	0.0
19	0.0	0.0	0.0	0.04000773482873356	0.0
20	0.0	0.0	0.0	0.04334171273112802	0.0
21	0.0	0.0	0.0	0.047342486214001375	0.0
22	0.0	0.0	0.0	0.05000966853591694	0.0
23	0.0	0.0	0.0	0.05201005527735362	0.0
24	0.0	0.0	0.0	0.05734441992118476	0.0
25	0.0	0.0	0.0	0.06467917130645258	0.0
26	0.0	0.0	0.0	0.06867994478932593	0.0
27	0.0	0.0	0.0	0.12402397796907402	0.0
28	0.0	0.0	0.0	0.19870508298271	0.0
29	0.0	0.0	0.0	0.2813877349620927	0.0
30	0.0	0.0	0.0	0.3440665195271086	0.0
31	0.0	0.0	0.0	0.41941442012122343	0.0
32	0.0	0.0	0.0	0.5221009395149729	0.0
33	0.0	0.0	0.0	0.652126077708357	0.0
34	0.0	0.0	0.0	0.9301798347680551	0.0
35	0.0	0.0	0.0	1.2962506084509673	0.0
36	0.0	0.0	0.0	1.8276866860926446	0.0
37	0.0	0.0	0.0	2.5224876809516505	0.0
38	0.0	0.0	0.0	3.264631162024658	0.0
39	0.0	0.0	0.0	3.9887711624247353	0.0
40	0.0	0.0	0.0	4.685572544025178	0.0
41	0.0	0.0	0.0	5.331030665928746	0.0
42	0.0	0.0	0.0	6.074507738162711	0.0
43	0.0	0.0	0.0	6.799981329723747	0.0
44	0.0	0.0	0.0	7.518120169899514	0.0
45	0.0	0.0	0.0	8.338278733888552	0.0
46	0.0	0.0	0.0	9.208446966413506	0.0
47	0.0	0.0	0.0	9.929919784491668	0.0
48	0.0	0.0	0.0	10.63272232631642	0.0
49	0.0	0.0	0.0	11.310853431663455	0.0
50	0.0	0.0	0.0	11.93430729941122	0.0
51	0.0	0.0	0.0	12.533089730681265	0.0
52	0.0	0.0	0.0	13.131205366370832	0.0
53	0.0	0.0	0.0	13.630635256149523	0.0
54	0.0	0.0	0.0	14.178741223303172	0.0
55	0.0	0.0	0.0	14.735515533003047	0.0
56	0.0	0.0	0.0	15.312960505697768	0.0
57	0.0	0.0	0.0	15.87773636236339	0.0
58	0.0	0.0	0.0	16.459182108540986	0.0
59	0.0	0.0	0.0	16.976615478992606	0.0
60	0.0	0.0	0.0	17.415366970947716	0.0
61	0.0	0.0	0.0	17.882790672863422	0.0
62	0.0	0.0	0.0	18.296203932760335	0.0
63	0.0	0.0	0.0	18.77829713744657	0.0
64	0.0	0.0	0.0	19.26772509351808	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACTCG	15	0.0022211228	70.00001	6
TCTTTAT	110	0.0	57.272724	1
CCCCTTG	20	0.006940313	52.5	19
AAGACAG	20	0.006940313	52.5	54
TATAGGG	20	0.006940313	52.5	5
GCTAATC	20	0.006940313	52.5	33
TCTATAG	20	0.006940313	52.5	3
CTATACA	115	0.0	51.73913	1
GTATGCC	3380	0.0	48.15089	42
CGTATGC	3405	0.0	48.105724	41
TCGTATG	3405	0.0	48.105724	40
CTCGTAT	3375	0.0	48.014816	39
TCTCGTA	3305	0.0	47.972767	38
TTGAAAA	3400	0.0	47.97059	58
TCTTCTG	3365	0.0	47.949482	50
CTTGAAA	3410	0.0	47.932552	57
AATCTCG	3255	0.0	47.84946	36
CCGTCTT	3365	0.0	47.84547	47
TGCTTGA	3410	0.0	47.829914	55
GTCTTCT	3385	0.0	47.76957	49
>>END_MODULE