FastQCFastQC Report
Wed 25 May 2016
SRR1779712_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779712_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163079
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTC150.002222013269.99570515
TTGCAGA150.002222013269.99570532
ATCTCGG1050.056.66319342
GTGTACA252.3856583E-455.9965736
TCTCGGT1250.053.19673543
GAGCTAT200.006943080652.4967811
GTATCGC200.006943080652.4967823
ATCGGAC506.160553E-848.99699810
GTAGATC751.4551915E-1146.66380738
GATCTCG1000.045.4972141
CGTCATC404.5790526E-543.7473149
CTCGGTG1450.043.44561444
AGATCTC901.8189894E-1242.77515840
CTTCCGA602.582783E-740.83082618
CATCGGA602.582783E-740.8308269
TAATGGC350.001252980439.99754754
AGTGTAC350.001252980439.99754735
CCGACTA801.4097168E-939.37258521
TCCGACT459.170512E-538.8865120
CTCGGTT459.170512E-538.8865144