##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779712_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	163079
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.48651880376995	34.0	27.0	34.0	12.0	34.0
2	27.989833148351412	34.0	23.0	34.0	12.0	34.0
3	26.94140876507705	32.0	21.0	34.0	11.0	34.0
4	29.23040366938723	34.0	23.0	34.0	12.0	34.0
5	28.87668553277859	34.0	23.0	34.0	11.0	34.0
6	31.20898460255459	37.0	27.0	38.0	11.0	38.0
7	32.235352191269264	37.0	31.0	38.0	12.0	38.0
8	31.460145083057906	37.0	27.0	38.0	11.0	38.0
9	32.85147689156789	37.0	32.0	38.0	12.0	38.0
10-11	32.57937257402854	37.0	31.0	38.0	11.0	38.0
12-13	31.595104213295393	37.0	29.0	38.0	11.0	38.0
14-15	31.625515854279215	36.5	29.0	38.0	11.5	38.0
16-17	31.277991035019838	36.5	27.5	38.0	11.0	38.0
18-19	31.89135020450211	37.0	29.0	38.0	11.5	38.0
20-21	30.77485758436095	35.5	27.0	38.0	11.0	38.0
22-23	30.774011368723137	35.5	26.5	38.0	11.0	38.0
24-25	31.961031156678665	36.5	29.0	38.0	11.0	38.0
26-27	30.53625850048136	36.5	25.0	38.0	11.0	38.0
28-29	32.60881229342834	37.0	31.5	38.0	11.0	38.0
30-31	33.061703223591024	38.0	34.0	38.0	11.0	38.0
32-33	33.152266692829855	38.0	34.0	38.0	11.0	38.0
34-35	33.21490811201933	38.0	34.0	38.0	11.0	38.0
36-37	31.14092249768517	36.5	27.5	38.0	11.0	38.0
38-39	29.418205287008135	34.5	23.0	37.5	11.0	38.0
40-41	30.58660219893426	35.0	26.0	38.0	11.0	38.0
42-43	31.642498421010675	36.5	28.5	38.0	11.0	38.0
44-45	33.22604381925325	37.5	34.0	38.0	21.0	38.0
46-47	31.82241735600537	37.0	29.0	38.0	15.5	38.0
48-49	31.28562537175234	36.5	27.5	38.0	11.0	38.0
50-51	29.130280416239984	34.0	22.5	37.5	11.0	38.0
52-53	32.453712617811	37.0	32.5	38.0	11.0	38.0
54-55	31.521324020873323	36.5	28.0	38.0	11.0	38.0
56-57	31.91379331489646	37.0	29.5	38.0	11.0	38.0
58-59	33.016921246757704	37.0	34.0	38.0	11.0	38.0
60-61	33.4606969628217	38.0	34.0	38.0	21.0	38.0
62-63	33.3296193869229	38.0	34.0	38.0	16.0	38.0
64-65	33.043696613297854	38.0	33.0	38.0	15.5	38.0
66-67	31.018766977967736	36.5	25.5	38.0	11.0	38.0
68-69	31.09014955941599	36.5	26.0	38.0	11.0	38.0
70-71	30.684821466896413	36.5	25.5	38.0	11.0	38.0
72-73	29.489011460703097	34.5	23.5	38.0	11.0	38.0
74-75	31.622784049448427	36.5	28.0	38.0	11.0	38.0
76	32.03348070567026	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	0.0
6	0.0
7	0.0
8	1.0
9	1.0
10	1.0
11	10.0
12	43.0
13	239.0
14	677.0
15	1222.0
16	1965.0
17	3032.0
18	3971.0
19	4215.0
20	3770.0
21	3086.0
22	2557.0
23	2271.0
24	2276.0
25	2371.0
26	2646.0
27	3088.0
28	3671.0
29	4537.0
30	5510.0
31	7093.0
32	9302.0
33	12561.0
34	17215.0
35	23122.0
36	26995.0
37	15629.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.6336806087847	17.69878402492044	11.039434875121874	30.628100491172987
2	21.15784374444288	16.714598446151864	38.39856756541308	23.728990243992175
3	20.607803579860068	17.568172480822177	36.00463579001588	25.81938814930187
4	14.550616572336105	18.978531877188356	32.70684760146922	33.76400394900631
5	14.921602413554167	29.163779517902366	38.53408470741175	17.38053336113172
6	28.093745975876722	32.42048332403314	17.046952703904243	22.438817996185897
7	27.708656540695	26.554001434887386	19.97620785018304	25.761134174234574
8	23.87829062344782	32.14493766748223	17.730274655530824	26.246497053539127
9	23.765460487990335	16.463388460665822	19.157821151971156	40.613329899372694
10-11	25.02920462245552	23.693917160053594	26.66987585352617	24.607002363964714
12-13	24.61704502250512	20.11694077535352	28.27121429535058	26.994799906790785
14-15	23.657987686722755	22.327932988299935	23.836432093011847	30.177647231965466
16-17	24.862252829297756	23.022864343949394	26.04579001106883	26.069092815684016
18-19	22.45146375265217	23.068668211977382	27.613844021732465	26.86602401363798
20-21	24.215396502244353	22.75626088449557	27.16616792170522	25.862174691554856
22-23	24.91361257347147	21.67856825296569	27.700392767678377	25.707426405884465
24-25	22.67456477614869	24.74321946074554	24.19562537942199	28.386590383683778
26-27	23.575600558016895	23.5648694639052	27.186767027946836	25.672762950131073
28-29	23.5488361254875	24.64249797640364	25.930241114572343	25.878424783536513
30-31	22.881706715070198	24.593511514746417	26.26239088509169	26.26239088509169
32-33	23.310818061683463	24.65284271912531	25.885714811328565	26.150624407862665
34-35	23.432469722520313	24.263988962133986	26.082170780315806	26.22137053502989
36-37	24.00972567584555	22.055085590239983	29.763879477658843	24.171309256255615
38-39	23.915203100470958	21.088537578492936	32.771107240973315	22.22515208006279
40-41	23.549738155683922	23.155438635220822	25.632534922795784	27.662288286299468
42-43	21.1810335576643	23.49803007772378	26.96386687311248	28.35706949149944
44-45	21.365453720396875	22.565522400873224	29.07207770705324	26.99694617167666
46-47	23.109706180841506	21.66713168359635	29.685760977210084	25.537401158352065
48-49	23.042299814808	22.13228350319487	29.92598452236408	24.89943215963305
50-51	24.22451088306265	21.05142128291057	31.821652067000052	22.902415767026728
52-53	20.126813982651996	23.725804621842297	29.219646355784356	26.92773503972135
54-55	21.817395783509326	22.828899763297645	28.70138710033482	26.652317352858212
56-57	22.001465578826984	23.159211530855337	27.850596809453286	26.988726080864385
58-59	20.00723600094436	24.05663703790009	28.550316268431107	27.385810692724444
60-61	20.179612811440276	23.369901823109895	28.49153446615933	27.958950899290503
62-63	19.950819722395117	24.271264184554816	27.89141092820109	27.88650516484898
64-65	20.83526241073865	24.35175333973123	26.874526673391607	27.938457576138514
66-67	19.65192968685323	24.49860643096084	23.946391284751073	31.90307259743485
68-69	20.07679379761277	24.82181630825472	23.96064624556841	31.1407436485641
70-71	20.252834654843596	25.040933090494327	23.45113478178217	31.25509747287991
72-73	19.951249425111143	24.818948336654913	22.61658745975778	32.61321477847616
74-75	21.478592540809693	25.897445331567265	25.108233072102237	27.515729055520804
76	21.961943424110675	25.33773218129304	25.469574975624415	27.23074941897187
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	1.0
14	3.0
15	5.0
16	4.5
17	3.0
18	5.5
19	19.0
20	42.5
21	55.0
22	65.0
23	100.0
24	152.5
25	180.0
26	239.5
27	397.5
28	566.5
29	637.0
30	771.0
31	1107.0
32	1572.5
33	1836.0
34	2126.5
35	2826.0
36	3590.5
37	3946.0
38	4516.0
39	5734.0
40	6833.0
41	7831.5
42	8379.0
43	8598.0
44	9189.5
45	9738.5
46	9915.0
47	9842.5
48	9537.5
49	8958.0
50	8611.0
51	8135.0
52	7241.5
53	6386.0
54	5948.0
55	5606.5
56	4844.5
57	4161.0
58	3898.0
59	3624.0
60	3154.0
61	2863.5
62	2769.0
63	2647.0
64	2431.5
65	2236.0
66	2130.0
67	2126.0
68	2014.5
69	1855.5
70	1682.0
71	1556.0
72	1487.5
73	1356.0
74	1185.5
75	1078.0
76	982.5
77	806.0
78	622.0
79	519.0
80	443.0
81	301.0
82	197.0
83	159.0
84	125.0
85	78.0
86	51.0
87	37.0
88	27.0
89	13.0
90	6.5
91	3.0
92	2.0
93	2.5
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0012263994751010247
9	0.0012263994751010247
10-11	0.003372598556527818
12-13	0.0030659986877525614
14-15	0.0018395992126515371
16-17	0.005212197769179355
18-19	0.0030659986877525614
20-21	0.0018395992126515371
22-23	0.003372598556527818
24-25	0.0012263994751010247
26-27	9.197996063257686E-4
28-29	0.0018395992126515371
30-31	0.003372598556527818
32-33	0.0027593988189773054
34-35	0.0024527989502020494
36-37	0.003372598556527818
38-39	0.004292398162853587
40-41	0.0030659986877525614
42-43	9.197996063257686E-4
44-45	0.0030659986877525614
46-47	0.0015329993438762807
48-49	0.0030659986877525614
50-51	0.0027593988189773054
52-53	0.003372598556527818
54-55	0.0030659986877525614
56-57	0.0021461990814267934
58-59	0.003372598556527818
60-61	0.0036791984253030743
62-63	0.003372598556527818
64-65	0.0027593988189773054
66-67	0.005212197769179355
68-69	0.027593988189773058
70-71	0.004905597900404099
72-73	0.0024527989502020494
74-75	0.0030659986877525614
76	0.0036791984253030743
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	163079.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.60953194148671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.19551763391183	86.29207281428721
2	4.110340935742451	7.530928184865969
3	0.9510836283604696	2.613849780939405
4	0.3479981015456819	1.2751977279650353
5	0.18306591839440042	0.8385291549274712
6	0.09066636473353215	0.4983541941644999
7	0.05073888090584047	0.32537155917132177
8	0.026847541261982563	0.19675925510319794
9	0.020877409303017173	0.17213127249602192
>10	0.022863585840797982	0.2568060560798546
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001839599212651537	0.0
2	0.0	0.0	0.0	0.003679198425303074	0.0
3	0.0	0.0	0.0	0.003679198425303074	0.0
4	0.0	0.0	0.0	0.006745197113055636	0.0
5	0.0	0.0	0.0	0.006745197113055636	0.0
6	0.0	0.0	0.0	0.007358396850606148	0.0
7	0.0	0.0	0.0	0.009197996063257685	0.0
8	0.0	0.0	0.0	0.009197996063257685	0.0
9	0.0	0.0	0.0	0.014716793701212295	0.0
10	0.0	0.0	0.0	0.01778279238896486	0.0
11	0.0	0.0	0.0	0.019009191864065883	0.0
12	0.0	0.0	0.0	0.019009191864065883	0.0
13	0.0	0.0	0.0	0.019009191864065883	0.0
14	0.0	0.0	0.0	0.019009191864065883	0.0
15	0.0	0.0	0.0	0.019009191864065883	0.0
16	0.0	0.0	0.0	0.019622391601616395	0.0
17	0.0	0.0	0.0	0.020235591339166907	0.0
18	0.0	0.0	0.0	0.022688390289368955	0.0
19	0.0	0.0	0.0	0.023301590026919467	0.0
20	0.0	0.0	0.0	0.02391478976446998	0.0
21	0.0	0.0	0.0	0.02575438897712152	0.0
22	0.0	0.0	0.0	0.038631583465682275	0.0
23	0.0	0.0	0.0	0.04108438241588432	0.0
24	0.0	0.0	0.0	0.04721637979138945	0.0
25	0.0	0.0	0.0	0.05150877795424304	0.0
26	0.0	0.0	0.0	0.06070677401750072	0.0
27	0.0	0.0	0.0	0.099338357483183	0.0
28	0.0	0.0	0.0	0.13613034173621374	0.0
29	0.0	0.0	0.0	0.1594319317631332	0.0
30	0.0	0.0	0.0	0.1943843168035124	0.0
31	0.0	0.0	0.0	0.21339350866757828	0.0
32	0.0	0.0	0.0	0.25447789108346264	0.0
33	0.0	0.0	0.0	0.2894302761238418	0.0
34	0.0	0.0	0.0	0.33664665591523124	0.0
35	0.0	0.0	0.0	0.46112620263798526	0.0
36	0.0	0.0	0.0	0.5610777598587188	0.0
37	0.0	0.0	0.0	0.8738096260094801	0.0
38	0.0	0.0	0.0	1.193286689273297	0.0
39	0.0	0.0	0.0	1.5869609207807258	0.0
40	0.0	0.0	0.0	2.0548323205317667	0.0
41	0.0	0.0	0.0	2.673550855720234	0.0
42	0.0	0.0	0.0	3.3787305539033228	0.0
43	0.0	0.0	0.0	4.127447433452499	0.0
44	0.0	0.0	0.0	4.772533557355637	0.0
45	0.0	0.0	0.0	5.245310555007083	0.0
46	0.0	0.0	0.0	5.768369931137669	0.0
47	0.0	0.0	0.0	6.348456882860454	0.0
48	0.0	0.0	0.0	6.789347494159273	0.0
49	0.0	0.0	0.0	7.166465332752837	0.0
50	0.0	0.0	0.0	7.733061890249511	0.0
51	0.0	0.0	0.0	8.379987613365302	0.0
52	0.0	0.0	0.0	9.017102140680283	0.0
53	0.0	0.0	0.0	9.846148185848577	0.0
54	0.0	0.0	0.0	10.583827470121843	0.0
55	0.0	0.0	0.0	11.092170052551218	0.0
56	0.0	0.0	0.0	11.56249425125246	0.0
57	0.0	0.0	0.0	11.988668068850066	0.0
58	0.0	0.0	0.0	12.322248726077545	0.0
59	0.0	0.0	0.0	12.775403332127373	0.0
60	0.0	0.0	0.0	13.048277215337352	0.0
61	0.0	0.0	0.0	13.306434304846118	0.0
62	0.0	0.0	0.0	13.510629817450438	0.0
63	0.0	0.0	0.0	13.814163687537942	0.0
64	0.0	0.0	0.0	14.118310757362996	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGTC	15	0.0022220132	69.995705	15
TTGCAGA	15	0.0022220132	69.995705	32
ATCTCGG	105	0.0	56.663193	42
GTGTACA	25	2.3856583E-4	55.99657	36
TCTCGGT	125	0.0	53.196735	43
GAGCTAT	20	0.0069430806	52.49678	11
GTATCGC	20	0.0069430806	52.49678	23
ATCGGAC	50	6.160553E-8	48.996998	10
GTAGATC	75	1.4551915E-11	46.663807	38
GATCTCG	100	0.0	45.49721	41
CGTCATC	40	4.5790526E-5	43.747314	9
CTCGGTG	145	0.0	43.445614	44
AGATCTC	90	1.8189894E-12	42.775158	40
CTTCCGA	60	2.582783E-7	40.830826	18
CATCGGA	60	2.582783E-7	40.830826	9
TAATGGC	35	0.0012529804	39.997547	54
AGTGTAC	35	0.0012529804	39.997547	35
CCGACTA	80	1.4097168E-9	39.372585	21
TCCGACT	45	9.170512E-5	38.88651	20
CTCGGTT	45	9.170512E-5	38.88651	44
>>END_MODULE