FastQCFastQC Report
Wed 25 May 2016
SRR1779712_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779712_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163079
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT158099.694074650936049No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG20381.249701065127944No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC11520.7064060976581902No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT7240.44395660998657094Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC6110.37466503964336306No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC3640.22320470446838647No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC2510.1539131341251786No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG2080.12754554541050656No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT1780.1091495532839912No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCAAC150.002221472370.0000142
CAATACG150.002221472370.0000169
ATATCGT308.348017E-658.33333637
ATCGTAT252.3849361E-455.99999639
TCGTTGT200.006941396352.534
AGTTTCC200.006941396352.533
CCTACCG200.006941396352.551
CCTGTCA200.006941396352.510
AATGCGG200.006941396352.511
CTTGATA200.006941396352.528
GATGATC200.006941396352.54
CCGTCTT25050.050.8582847
CGTATGC25100.050.7569741
TATGCCG25100.050.7569743
TCGTATG25100.050.7569740
ATGCCGT25100.050.7569744
GCCGTCT25100.050.7569746
CGTCTTC25150.050.65606348
TGCCGTC25300.050.63241245
TCTCGTA24350.050.59548638