##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779712_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	163079
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.57505871387487	34.0	34.0	34.0	34.0	34.0
2	33.60381164956861	34.0	34.0	34.0	34.0	34.0
3	33.634030132635104	34.0	34.0	34.0	34.0	34.0
4	33.67688666229251	34.0	34.0	34.0	34.0	34.0
5	33.669945241263434	34.0	34.0	34.0	34.0	34.0
6	37.289559048068725	38.0	38.0	38.0	36.0	38.0
7	37.2731927470735	38.0	38.0	38.0	36.0	38.0
8	37.13346292287787	38.0	38.0	38.0	36.0	38.0
9	37.1493509280778	38.0	38.0	38.0	36.0	38.0
10-11	37.062236707362686	38.0	38.0	38.0	36.0	38.0
12-13	37.043672085308344	38.0	38.0	38.0	35.5	38.0
14-15	37.06192397549654	38.0	38.0	38.0	36.0	38.0
16-17	37.03432998730676	38.0	38.0	38.0	35.5	38.0
18-19	37.02728125632362	38.0	38.0	38.0	35.0	38.0
20-21	37.045474892536745	38.0	38.0	38.0	35.5	38.0
22-23	36.98249927949031	38.0	37.5	38.0	35.0	38.0
24-25	36.96509973693732	38.0	37.0	38.0	35.0	38.0
26-27	36.886006168789365	38.0	37.5	38.0	35.0	38.0
28-29	36.84282157727236	38.0	37.0	38.0	35.0	38.0
30-31	36.729207929899005	38.0	37.0	38.0	34.5	38.0
32-33	36.70671576352565	38.0	37.0	38.0	34.0	38.0
34-35	36.61724072382097	38.0	37.0	38.0	34.0	38.0
36-37	36.75551726463861	38.0	37.0	38.0	34.5	38.0
38-39	36.41594564597527	38.0	37.0	38.0	34.0	38.0
40-41	36.70120309788507	38.0	37.0	38.0	34.0	38.0
42-43	36.825924858504166	38.0	37.0	38.0	35.0	38.0
44-45	36.818170947822836	38.0	37.0	38.0	35.0	38.0
46-47	36.83181464198333	38.0	37.0	38.0	35.0	38.0
48-49	36.81488726322825	38.0	37.0	38.0	35.0	38.0
50-51	36.769602462610145	38.0	37.0	38.0	34.0	38.0
52-53	36.74576738881156	38.0	37.0	38.0	34.0	38.0
54-55	36.736293452866406	38.0	37.0	38.0	34.0	38.0
56-57	36.76026956260463	38.0	37.0	38.0	34.5	38.0
58-59	36.71445741021223	38.0	37.0	38.0	34.0	38.0
60-61	36.7766235996051	38.0	37.0	38.0	34.5	38.0
62-63	36.77708656540695	38.0	37.0	38.0	35.0	38.0
64-65	36.78781449481539	38.0	37.0	38.0	35.0	38.0
66-67	36.7294593417914	38.0	37.0	38.0	34.0	38.0
68-69	36.58494349364419	38.0	37.0	38.0	34.0	38.0
70-71	35.707951360996816	38.0	37.0	38.0	29.5	38.0
72-73	34.27675237154999	38.0	36.0	38.0	22.0	38.0
74-75	34.12455619668995	38.0	36.0	38.0	21.0	38.0
76	33.67325038784883	38.0	34.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	8.0
22	14.0
23	28.0
24	61.0
25	97.0
26	180.0
27	334.0
28	618.0
29	1032.0
30	1517.0
31	2401.0
32	3882.0
33	6011.0
34	10198.0
35	18960.0
36	26090.0
37	91645.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.600794706859865	16.371206593123578	11.205612003998063	34.82238669601849
2	19.65243838875637	16.666768866622924	38.94247573262039	24.73831701200032
3	18.51495287560017	18.6682528099878	36.01138098712894	26.805413327283095
4	13.55110100012877	24.122051275762054	32.57500965789586	29.75183806621331
5	13.712372531104558	30.62748729143544	40.71094377571606	14.949196401743938
6	28.3604878617112	41.25791794161112	16.076257519361782	14.305336677315902
7	26.911496881879334	26.290325547740668	18.716082389516735	28.082095180863263
8	23.692811459476694	39.889256127398376	16.328895811232595	20.089036601892335
9	21.99976698409973	15.983664358991653	17.132187467423766	44.88438118948485
10-11	23.727763844517074	27.016047437131697	27.099749201307343	22.156439517043886
12-13	23.49352154477278	19.05824784306992	28.174381741364613	29.273848870792683
14-15	21.557343373457037	19.50251105292527	20.633251368968413	38.306894204649275
16-17	27.842334083481013	27.599200387542233	20.34780689113865	24.210658637838105
18-19	28.01127061117618	22.954212375597102	21.452179618467124	27.58233739475959
20-21	21.589536359678437	21.977998393416687	21.311756878568055	35.120708368336814
22-23	22.302687654449684	27.806155298965535	21.04532159260236	28.845835453982428
24-25	27.34840169488407	28.083934780075914	21.38135504878004	23.186308476259974
26-27	27.49740923110885	28.56376357470919	22.114128735152903	21.82469845902906
28-29	22.048516363234995	22.799686041734375	27.76997651445005	27.381821080580576
30-31	26.677254582135042	21.516872190778702	29.580755339436713	22.225117887649546
32-33	22.24351387977606	22.199670098541198	27.9763182261358	27.580497795546943
34-35	21.578805364271304	33.04349425738446	23.671962668399978	21.70573770994426
36-37	27.28524212191637	28.75262909387475	22.06200675746111	21.900122026747773
38-39	21.453712617811	23.174351081377736	28.33626647207795	27.03566982873331
40-41	21.077821178692535	20.903365853359414	29.530779560826346	28.488033407121705
42-43	26.636170199719157	20.82211688813397	29.145076925907077	23.396635986239797
44-45	20.83315448340988	26.483176865200303	30.523856535789402	22.159812115600413
46-47	25.98679167765316	21.108481165570062	25.34170555375002	27.563021603026755
48-49	25.270574384194163	20.514903819621168	24.568767284567603	29.645754511617067
50-51	20.4364142532147	21.015888005199933	30.43279637476315	28.114901366822213
52-53	18.585777445287253	21.023246402050543	36.5954537371458	23.795522415516405
54-55	18.30155936693259	21.327393471875595	31.446108941065372	28.92493822012644
56-57	23.995425529957874	21.055745988140718	25.738445783945203	29.210382697956206
58-59	18.738770779806107	21.335058468594976	35.08728898264032	24.838881768958604
60-61	23.950048749379135	27.074914611936546	25.371139141152447	23.60389749753187
62-63	18.20068801010553	35.04651120009321	22.633815512726958	24.1189852770743
64-65	18.310450763127072	36.12022394054416	21.483759404950973	24.0855658913778
66-67	18.35276154501806	35.93841021836043	21.144966549954315	24.5638616866672
68-69	18.18597121640432	35.99175859552732	21.090698373181098	24.731571814887264
70-71	18.518325474156697	34.34163810177889	21.73026569944628	25.40977072461813
72-73	19.41850268888085	30.88963017923828	22.719050276246485	26.972816855634385
74-75	19.43321948258206	29.78740365099124	23.685453062626088	27.093923803800614
76	19.442110878776546	29.834620030782627	23.462861557895252	27.260407532545578
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	2.5
16	5.5
17	6.0
18	13.5
19	28.0
20	49.0
21	63.0
22	68.0
23	89.5
24	177.5
25	249.0
26	316.5
27	435.5
28	579.5
29	672.0
30	801.5
31	1058.5
32	1594.0
33	2002.0
34	2226.0
35	2691.5
36	3340.5
37	3748.0
38	4238.5
39	8267.5
40	12687.5
41	12940.5
42	12312.0
43	11374.0
44	9942.0
45	8932.5
46	8417.0
47	8402.0
48	7896.0
49	7255.0
50	7105.0
51	6697.0
52	5655.0
53	4791.0
54	4561.0
55	4283.0
56	3674.5
57	3058.5
58	2773.0
59	2584.0
60	2361.5
61	2269.5
62	2211.0
63	2052.0
64	1893.0
65	1952.0
66	1933.5
67	1856.0
68	1764.5
69	1669.0
70	1620.0
71	1575.0
72	1564.0
73	1431.0
74	1313.0
75	1317.0
76	1215.5
77	1022.0
78	820.5
79	711.0
80	620.5
81	478.0
82	360.0
83	294.0
84	235.5
85	146.0
86	89.5
87	64.0
88	51.0
89	28.5
90	17.0
91	9.5
92	4.0
93	3.5
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	163079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.21189730130796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	50.09652182747993	18.140901035694355
2	23.38876282724286	16.939029550095352
3	11.57753920140888	12.577339816898558
4	5.7032546482880075	8.261026864280502
5	2.7551054966640702	4.9883798649734175
6	1.5206421241575507	3.30392018592216
7	0.9533647170386427	2.4166201656865693
8	0.7247603887966946	2.0995959013729544
9	0.5655840417245233	1.8432784110768403
>10	2.67043722694483	15.446501388897405
>50	0.020320384732617606	0.472776997651445
>100	0.015240288549463204	1.0418263540983206
>500	0.003386730788769601	0.818621649629934
>1k	0.003386730788769601	1.9561071627861342
>5k	0.0	0.0
>10k+	0.0016933653943848005	9.694074650936049
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	15809	9.694074650936049	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	2038	1.249701065127944	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	1152	0.7064060976581902	No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT	724	0.44395660998657094	Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	611	0.37466503964336306	No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	364	0.22320470446838647	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	251	0.1539131341251786	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	208	0.12754554541050656	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT	178	0.1091495532839912	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001839599212651537	0.0
2	0.0	0.0	0.0	0.004905597900404099	0.0
3	0.0	0.0	0.0	0.004905597900404099	0.0
4	0.0	0.0	0.0	0.01042439553835871	0.0
5	0.0	0.0	0.0	0.011037595275909222	0.0
6	0.0	0.0	0.0	0.012263994751010247	0.0
7	0.0	0.0	0.0	0.014716793701212295	0.0
8	0.0	0.0	0.0	0.015329993438762807	0.0
9	0.0	0.0	0.0	0.022075190551818443	0.0
10	0.0	0.0	0.0	0.027593988189773055	0.0
11	0.0	0.0	0.0	0.02882038766487408	0.0
12	0.0	0.0	0.0	0.02882038766487408	0.0
13	0.0	0.0	0.0	0.030659986877525615	0.0
14	0.0	0.0	0.0	0.030659986877525615	0.0
15	0.0	0.0	0.0	0.03127318661507613	0.0
16	0.0	0.0	0.0	0.03372598556527818	0.0
17	0.0	0.0	0.0	0.03433918530282869	0.0
18	0.0	0.0	0.0	0.036178784515480227	0.0
19	0.0	0.0	0.0	0.03740518399058125	0.0
20	0.0	0.0	0.0	0.038631583465682275	0.0
21	0.0	0.0	0.0	0.042310781890985354	0.0
22	0.0	0.0	0.0	0.0447635808411874	0.0
23	0.0	0.0	0.0	0.05518797637954611	0.0
24	0.0	0.0	0.0	0.06070677401750072	0.0
25	0.0	0.0	0.0	0.06745197113055636	0.0
26	0.0	0.0	0.0	0.08032916561911711	0.0
27	0.0	0.0	0.0	0.13122474383580965	0.0
28	0.0	0.0	0.0	0.17660152441454754	0.0
29	0.0	0.0	0.0	0.22688390289368957	0.0
30	0.0	0.0	0.0	0.2790058805854831	0.0
31	0.0	0.0	0.0	0.3476842511911405	0.0
32	0.0	0.0	0.0	0.4120702236339443	0.0
33	0.0	0.0	0.0	0.4923993892530614	0.0
34	0.0	0.0	0.0	0.6107469386003103	0.0
35	0.0	0.0	0.0	0.8039048559287216	0.0
36	0.0	0.0	0.0	1.1289007168304932	0.0
37	0.0	0.0	0.0	1.6059701126447918	0.0
38	0.0	0.0	0.0	2.1517178790647478	0.0
39	0.0	0.0	0.0	2.747134824226295	0.0
40	0.0	0.0	0.0	3.422267735269409	0.0
41	0.0	0.0	0.0	4.229238589885884	0.0
42	0.0	0.0	0.0	5.239791757369128	0.0
43	0.0	0.0	0.0	6.262608919603382	0.0
44	0.0	0.0	0.0	7.09778696214718	0.0
45	0.0	0.0	0.0	8.000416975821535	0.0
46	0.0	0.0	0.0	8.828849821252277	0.0
47	0.0	0.0	0.0	9.643792272456908	0.0
48	0.0	0.0	0.0	10.444631129697877	0.0
49	0.0	0.0	0.0	11.194574408722152	0.0
50	0.0	0.0	0.0	11.899754106905242	0.0
51	0.0	0.0	0.0	12.654602983829923	0.0
52	0.0	0.0	0.0	13.541289804327963	0.0
53	0.0	0.0	0.0	14.435335021676611	0.0
54	0.0	0.0	0.0	15.293814654247328	0.0
55	0.0	0.0	0.0	16.097106310438498	0.0
56	0.0	0.0	0.0	16.786956015182824	0.0
57	0.0	0.0	0.0	17.48477731651531	0.0
58	0.0	0.0	0.0	18.090618657215213	0.0
59	0.0	0.0	0.0	18.75410077324487	0.0
60	0.0	0.0	0.0	19.346451719718665	0.0
61	0.0	0.0	0.0	19.921633073541045	0.0
62	0.0	0.0	0.0	20.54893640505522	0.0
63	0.0	0.0	0.0	21.182371733944898	0.0
64	0.0	0.0	0.0	21.863023442625966	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCAAC	15	0.0022214723	70.00001	42
CAATACG	15	0.0022214723	70.00001	69
ATATCGT	30	8.348017E-6	58.333336	37
ATCGTAT	25	2.3849361E-4	55.999996	39
TCGTTGT	20	0.0069413963	52.5	34
AGTTTCC	20	0.0069413963	52.5	33
CCTACCG	20	0.0069413963	52.5	51
CCTGTCA	20	0.0069413963	52.5	10
AATGCGG	20	0.0069413963	52.5	11
CTTGATA	20	0.0069413963	52.5	28
GATGATC	20	0.0069413963	52.5	4
CCGTCTT	2505	0.0	50.85828	47
CGTATGC	2510	0.0	50.75697	41
TATGCCG	2510	0.0	50.75697	43
TCGTATG	2510	0.0	50.75697	40
ATGCCGT	2510	0.0	50.75697	44
GCCGTCT	2510	0.0	50.75697	46
CGTCTTC	2515	0.0	50.656063	48
TGCCGTC	2530	0.0	50.632412	45
TCTCGTA	2435	0.0	50.595486	38
>>END_MODULE