FastQCFastQC Report
Wed 25 May 2016
SRR1779711_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779711_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43119
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGC252.7948408E-670.0000149
CTATGTG150.002210363770.032
AACGTGT150.002210363770.033
GGTGTTT150.002210363770.047
TAGACTC150.002210363770.039
CGATTGA150.002210363770.025
GATGGCT150.002210363770.070
GGTGGCT207.842491E-570.047
GGAGTGT150.002210363770.033
TATGTGC150.002210363770.033
TGCACGC150.002210363770.028
GTGTAGA750.060.6666636
TCTCGGT1100.060.45454443
TTGGTGG308.244622E-658.33333245
TGCCTAC308.244622E-658.33333219
GACGATT308.244622E-658.33333223
TCTGACT553.45608E-1157.27272411
CTGACTC553.45608E-1157.27272412
CTCGGTG1100.057.27272444
GTTTATA252.3652197E-456.00000436
Produced by FastQC (version 0.11.5)