FastQCFastQC Report
Wed 25 May 2016
SRR1779711_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779711_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43119
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT1017623.599805190287345No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG13393.105359586261277No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT6241.4471578654421484RNA PCR Primer, Index 30 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC6121.419327906491338No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC4200.9740485632783692No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC3390.786196340360398No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC2630.6099399336719311No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT1870.4336835269834644No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1530.3548319766228345No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC1320.30612954845891605No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT1320.30612954845891605No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT1130.2620654467867993RNA PCR Primer, Index 30 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1010.2342354878359888No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC930.21568218186878174No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT900.2087246921310791No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT660.15306477422945802No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG620.1437881212458545No Hit
TCTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT480.1113198358032422No Hit
CCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG450.10436234606553955No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGAC150.002210363770.028
AGCCAAT150.002210363770.041
AAACACG150.002210363770.069
GACGGAT150.002210363770.032
ACATTGT150.002210363770.027
AGACGTT150.002210363770.025
ATTGACG150.002210363770.029
TTTACAC401.3096724E-1070.02
AGACAAT150.002210363770.025
TTACACA453.7107384E-1062.2222253
TCTTTAT453.7107384E-1062.2222251
CTTTACA509.440555E-1056.0000041
CGAGACT252.3652197E-456.00000423
CTATACA452.6228008E-854.4444471
CATTGTC200.00690695152.528
CATTGCT200.00690695152.528
AATCTCG200.00690695152.536
CCGCCCA200.00690695152.543
ACATTGA200.00690695152.527
TTCCATT200.00690695152.529
Produced by FastQC (version 0.11.5)