##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779711_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43119
Sequences flagged as poor quality	0
Sequence length	76
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.431364363737565	34.0	34.0	34.0	34.0	34.0
2	33.48106403209722	34.0	34.0	34.0	34.0	34.0
3	33.51153783714836	34.0	34.0	34.0	34.0	34.0
4	33.54293930749785	34.0	34.0	34.0	34.0	34.0
5	33.55288851782277	34.0	34.0	34.0	34.0	34.0
6	37.055149701987524	38.0	37.0	38.0	35.0	38.0
7	37.07896750852292	38.0	37.0	38.0	36.0	38.0
8	36.89269231661217	38.0	37.0	38.0	35.0	38.0
9	36.90092534613512	38.0	37.0	38.0	35.0	38.0
10-11	36.74828961710615	38.0	37.0	38.0	34.0	38.0
12-13	36.74406873999861	38.0	37.0	38.0	34.0	38.0
14-15	36.777789373594004	38.0	37.0	38.0	34.0	38.0
16-17	36.564113267932925	38.0	37.0	38.0	34.0	38.0
18-19	36.55440756974883	38.0	37.0	38.0	34.0	38.0
20-21	36.726779378000415	38.0	37.0	38.0	34.0	38.0
22-23	36.64914538834388	38.0	37.0	38.0	34.0	38.0
24-25	36.569435747582276	38.0	37.0	38.0	34.0	38.0
26-27	36.25851712702057	38.0	36.5	38.0	33.5	38.0
28-29	36.18487209814699	38.0	37.0	38.0	33.0	38.0
30-31	36.24518193835664	38.0	37.0	38.0	34.0	38.0
32-33	35.594969734919644	38.0	36.5	38.0	31.0	38.0
34-35	35.545014958602934	38.0	36.0	38.0	31.0	38.0
36-37	35.58577425264964	38.0	36.0	38.0	31.0	38.0
38-39	34.26865187040516	37.0	34.0	38.0	24.5	38.0
40-41	35.64114427514553	38.0	36.5	38.0	31.5	38.0
42-43	36.14889028038684	38.0	37.0	38.0	34.0	38.0
44-45	36.11964563185603	38.0	37.0	38.0	33.5	38.0
46-47	36.078700804749644	38.0	37.0	38.0	33.0	38.0
48-49	36.07205640205014	38.0	37.0	38.0	32.5	38.0
50-51	35.88474918249496	38.0	36.5	38.0	31.0	38.0
52-53	35.879554256824136	38.0	37.0	38.0	31.5	38.0
54-55	35.886488554929386	38.0	37.0	38.0	31.5	38.0
56-57	35.832533222013495	38.0	37.0	38.0	31.5	38.0
58-59	35.6800018553306	38.0	36.0	38.0	31.0	38.0
60-61	35.652427004336836	38.0	36.0	38.0	31.0	38.0
62-63	35.8236508267817	38.0	36.5	38.0	31.5	38.0
64-65	35.97753890396345	38.0	37.0	38.0	32.0	38.0
66-67	35.851538764813654	38.0	36.5	38.0	31.0	38.0
68-69	35.380470326306266	38.0	36.0	38.0	29.5	38.0
70-71	33.11787147197291	37.5	31.5	38.0	18.0	38.0
72-73	29.215032816159926	36.5	21.0	38.0	11.0	38.0
74-75	28.75993181660057	36.0	19.0	38.0	11.0	38.0
76	28.281894292539253	34.0	19.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	0.0
21	2.0
22	7.0
23	17.0
24	33.0
25	71.0
26	170.0
27	254.0
28	469.0
29	695.0
30	997.0
31	1465.0
32	2119.0
33	3075.0
34	4931.0
35	8179.0
36	7177.0
37	13456.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.72979429022009	11.13430274357012	11.090238641898004	49.04566432431179
2	15.577819522716204	10.842088174586609	50.367587374475285	23.212504928221897
3	15.823650826781696	13.08471903337276	47.99044504742689	23.101185092418657
4	12.938611748881005	36.26011734966024	24.42774646907396	26.373524432384794
5	13.817574619077435	24.82896171061481	46.94682158677149	14.406642083536262
6	25.072473851434403	46.4412439991651	12.739163709733528	15.747118439666968
7	21.865071082353488	21.565899023632273	12.792504464389248	43.77652542962499
8	18.896542127600362	48.185254760082564	11.403325680094623	21.514877432222455
9	16.728124492683037	14.288364757995314	10.431596280062154	58.55191446925949
10-11	20.540828868944086	29.337415060646123	27.461211994712308	22.66054407569749
12-13	19.927410190403304	11.657274055520768	30.76022171200631	37.65509404206962
14-15	19.726802569632877	11.528560495373268	14.493610705257542	54.25102622973631
16-17	36.341288063266774	26.86402745889283	11.058929938078341	25.735754539762052
18-19	36.664811336069945	16.09383334492915	11.305920823766785	35.93543449523412
20-21	21.21338621025534	12.87251559637283	11.355782833553654	54.558315359818174
22-23	20.880586284468563	28.830677891416777	11.312878313504488	38.975857510610176
24-25	36.618428071151925	28.773858391892205	10.92325888819314	23.684454648762728
26-27	36.45376748069297	31.19158607574387	12.231266958881235	20.123379484681926
28-29	21.289918597370068	30.411187643498227	13.513764233864423	34.78512952526728
30-31	20.123379484681926	15.257774994781883	42.38850622695332	22.230339293582876
32-33	36.088499269463576	11.819615482733829	17.572299914190957	34.51958533361164
34-35	36.2775110740045	12.626684292307337	12.92585635102855	38.16994828265962
36-37	38.40882209698741	25.9827454254505	13.69813771191354	21.910294765648555
38-39	20.40051949256708	15.32850947378186	29.626150884760776	34.64482014889028
40-41	19.1249797073216	11.962244022356733	31.762100234235486	37.150676036086175
42-43	34.25520072357893	11.92513741042232	30.697604304366983	23.122057561631763
44-45	19.71288759015747	27.166678262482897	33.034161274612124	20.086272872747514
46-47	33.64642037152995	13.279528746028433	18.911616688698718	34.1624341937429
48-49	32.45321088151395	11.74192351399615	15.888587397666923	39.91627820682298
50-51	19.643312692780444	12.29852269301236	32.04851689510424	36.00964771910295
52-53	16.346622138732343	12.4028850390779	47.57067649991883	23.679816322270923
54-55	16.299079292191376	12.0074677056518	34.930077228136085	36.76337577402074
56-57	32.413785106333634	12.094436327373085	19.6491106008952	35.842667965398086
58-59	18.412996590830026	14.090076300470791	44.29253925183794	23.204387856861246
60-61	32.176070873628795	29.717757832973863	18.17992068461699	19.926250608780354
62-63	17.089913959043574	50.104362346065535	12.446949140750018	20.358774554140865
64-65	16.151812426076674	52.28321621558941	11.862520002782997	19.702451355550917
66-67	16.143695354716016	51.99216122822885	11.667710290127323	20.196433126927804
68-69	16.059045896240637	52.12783227811406	11.804540921635475	20.008580904009836
70-71	16.25037686402746	48.61893828706603	13.345624898536606	21.785059950369906
72-73	17.411118068600846	39.38866856838053	16.985551612978036	26.214661750040584
74-75	18.221665623043208	34.81643822908694	18.52199726338737	28.439898884482478
76	18.887265474616758	35.003130870381966	18.138175746190775	27.971427908810504
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	1.5
15	1.5
16	4.5
17	7.0
18	6.0
19	9.0
20	20.0
21	27.0
22	20.0
23	23.0
24	41.0
25	49.0
26	64.5
27	101.5
28	144.5
29	166.0
30	167.5
31	183.0
32	220.5
33	244.0
34	257.5
35	265.5
36	275.0
37	290.0
38	338.5
39	419.5
40	654.5
41	2660.5
42	4464.0
43	4972.0
44	4934.0
45	3545.5
46	2703.0
47	2069.0
48	1122.5
49	688.5
50	567.0
51	532.5
52	500.5
53	489.0
54	475.0
55	528.0
56	634.0
57	719.0
58	751.0
59	792.5
60	899.0
61	1002.0
62	1040.0
63	1040.5
64	1065.5
65	1142.0
66	1152.5
67	1111.0
68	1109.0
69	1075.0
70	1036.5
71	1030.0
72	1030.5
73	970.0
74	883.5
75	858.0
76	797.0
77	675.0
78	569.5
79	525.0
80	459.0
81	310.5
82	197.5
83	167.0
84	137.5
85	85.0
86	44.0
87	26.0
88	16.5
89	6.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	43119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.5353324520513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.21862348178138	29.699204527006657
2	21.382910718090773	18.618242538092254
3	6.914553590453868	9.030821679538024
4	2.21606648199446	3.8590876411790624
5	0.6179416151715321	1.3451146826225098
6	0.18644790112934156	0.4870242816391846
7	0.05859791178350735	0.17857556993436768
8	0.05859791178350735	0.20408636563927735
9	0.026635414447048797	0.10436234606553955
>10	0.22906456424461966	1.9133096778682253
>50	0.021308331557639035	0.7212597694751733
>100	0.047943746004687836	4.267260372457617
>500	0.010654165778819518	2.8664857719334864
>1k	0.005327082889409759	3.105359586261277
>5k	0.0	0.0
>10k+	0.005327082889409759	23.599805190287345
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	10176	23.599805190287345	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	1339	3.105359586261277	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT	624	1.4471578654421484	RNA PCR Primer, Index 30 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	612	1.419327906491338	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	420	0.9740485632783692	No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	339	0.786196340360398	No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	263	0.6099399336719311	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT	187	0.4336835269834644	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	153	0.3548319766228345	No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	132	0.30612954845891605	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT	132	0.30612954845891605	No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT	113	0.2620654467867993	RNA PCR Primer, Index 30 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	101	0.2342354878359888	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	93	0.21568218186878174	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT	90	0.2087246921310791	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT	66	0.15306477422945802	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG	62	0.1437881212458545	No Hit
TCTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	48	0.1113198358032422	No Hit
CCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	45	0.10436234606553955	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002319163245900879	0.0
2	0.0	0.0	0.0	0.002319163245900879	0.0
3	0.0	0.0	0.0	0.002319163245900879	0.0
4	0.0	0.0	0.0	0.004638326491801758	0.0
5	0.0	0.0	0.0	0.009276652983603516	0.0
6	0.0	0.0	0.0	0.009276652983603516	0.0
7	0.0	0.0	0.0	0.009276652983603516	0.0
8	0.0	0.0	0.0	0.013914979475405273	0.0
9	0.0	0.0	0.0	0.02087246921310791	0.0
10	0.0	0.0	0.0	0.02087246921310791	0.0
11	0.0	0.0	0.0	0.02319163245900879	0.0
12	0.0	0.0	0.0	0.02319163245900879	0.0
13	0.0	0.0	0.0	0.02319163245900879	0.0
14	0.0	0.0	0.0	0.02319163245900879	0.0
15	0.0	0.0	0.0	0.02319163245900879	0.0
16	0.0	0.0	0.0	0.02319163245900879	0.0
17	0.0	0.0	0.0	0.02319163245900879	0.0
18	0.0	0.0	0.0	0.037106611934414065	0.0
19	0.0	0.0	0.0	0.051021591409819336	0.0
20	0.0	0.0	0.0	0.05334075465572022	0.0
21	0.0	0.0	0.0	0.057979081147521976	0.0
22	0.0	0.0	0.0	0.06029824439342285	0.0
23	0.0	0.0	0.0	0.06029824439342285	0.0
24	0.0	0.0	0.0	0.06029824439342285	0.0
25	0.0	0.0	0.0	0.06261740763932373	0.0
26	0.0	0.0	0.0	0.06493657088522461	0.0
27	0.0	0.0	0.0	0.0881282033442334	0.0
28	0.0	0.0	0.0	0.15770310072125976	0.0
29	0.0	0.0	0.0	0.2574271202949976	0.0
30	0.0	0.0	0.0	0.3316403441638257	0.0
31	0.0	0.0	0.0	0.4058535680326538	0.0
32	0.0	0.0	0.0	0.4939817713768872	0.0
33	0.0	0.0	0.0	0.6632806883276514	0.0
34	0.0	0.0	0.0	0.9647719102947656	0.0
35	0.0	0.0	0.0	1.3172847236716994	0.0
36	0.0	0.0	0.0	1.8135856582944874	0.0
37	0.0	0.0	0.0	2.5186112850483546	0.0
38	0.0	0.0	0.0	3.2537860339989333	0.0
39	0.0	0.0	0.0	3.873002620654468	0.0
40	0.0	0.0	0.0	4.496857533801804	0.0
41	0.0	0.0	0.0	5.058095039309817	0.0
42	0.0	0.0	0.0	5.760801502817784	0.0
43	0.0	0.0	0.0	6.417124701407732	0.0
44	0.0	0.0	0.0	7.061852083768176	0.0
45	0.0	0.0	0.0	7.667153690948306	0.0
46	0.0	0.0	0.0	8.242306175931724	0.0
47	0.0	0.0	0.0	8.852246109603655	0.0
48	0.0	0.0	0.0	9.302163779308426	0.0
49	0.0	0.0	0.0	9.805422203668916	0.0
50	0.0	0.0	0.0	10.304042301537605	0.0
51	0.0	0.0	0.0	10.716853359307962	0.0
52	0.0	0.0	0.0	11.067047009438994	0.0
53	0.0	0.0	0.0	11.438113128783135	0.0
54	0.0	0.0	0.0	11.832370880586284	0.0
55	0.0	0.0	0.0	12.21271365291403	0.0
56	0.0	0.0	0.0	12.651035506389295	0.0
57	0.0	0.0	0.0	13.024420788979336	0.0
58	0.0	0.0	0.0	13.420997704028387	0.0
59	0.0	0.0	0.0	13.706254783274195	0.0
60	0.0	0.0	0.0	14.021660984716714	0.0
61	0.0	0.0	0.0	14.309237227208424	0.0
62	0.0	0.0	0.0	14.580579326978826	0.0
63	0.0	0.0	0.0	15.000347874486884	0.0
64	0.0	0.0	0.0	15.389967299798233	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGAC	15	0.0022103637	70.0	28
AGCCAAT	15	0.0022103637	70.0	41
AAACACG	15	0.0022103637	70.0	69
GACGGAT	15	0.0022103637	70.0	32
ACATTGT	15	0.0022103637	70.0	27
AGACGTT	15	0.0022103637	70.0	25
ATTGACG	15	0.0022103637	70.0	29
TTTACAC	40	1.3096724E-10	70.0	2
AGACAAT	15	0.0022103637	70.0	25
TTACACA	45	3.7107384E-10	62.222225	3
TCTTTAT	45	3.7107384E-10	62.222225	1
CTTTACA	50	9.440555E-10	56.000004	1
CGAGACT	25	2.3652197E-4	56.000004	23
CTATACA	45	2.6228008E-8	54.444447	1
CATTGTC	20	0.006906951	52.5	28
CATTGCT	20	0.006906951	52.5	28
AATCTCG	20	0.006906951	52.5	36
CCGCCCA	20	0.006906951	52.5	43
ACATTGA	20	0.006906951	52.5	27
TTCCATT	20	0.006906951	52.5	29
>>END_MODULE