##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779710_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	196351
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.175838167363548	34.0	23.0	34.0	12.0	34.0
2	27.70137152344526	34.0	23.0	34.0	12.0	34.0
3	26.695458642940448	32.0	21.0	34.0	11.0	34.0
4	28.91001828358399	34.0	23.0	34.0	12.0	34.0
5	28.540898696721687	34.0	23.0	34.0	11.0	34.0
6	30.842430137865353	36.0	26.0	38.0	11.0	38.0
7	31.777159270897524	37.0	27.0	38.0	12.0	38.0
8	31.01612418576936	37.0	26.0	38.0	11.0	38.0
9	32.44230994494553	37.0	31.0	38.0	12.0	38.0
10-11	32.129660658718315	37.0	31.0	38.0	11.0	38.0
12-13	31.21637017382137	36.0	27.5	38.0	11.0	38.0
14-15	31.167788806779697	36.5	26.5	38.0	11.0	38.0
16-17	30.86625736563603	36.0	27.0	38.0	11.0	38.0
18-19	31.47046360853777	37.0	27.5	38.0	11.5	38.0
20-21	30.331149319331196	35.5	25.0	38.0	11.0	38.0
22-23	30.34609958696416	35.5	26.0	38.0	11.0	38.0
24-25	31.480455918228074	36.5	28.5	38.0	11.0	38.0
26-27	30.064776853695676	35.0	24.0	38.0	11.0	38.0
28-29	32.11581555479728	37.0	31.0	38.0	11.0	38.0
30-31	32.56584891342545	37.0	31.5	38.0	11.0	38.0
32-33	32.67817581779568	38.0	32.0	38.0	11.0	38.0
34-35	32.7243991627239	38.0	32.5	38.0	11.0	38.0
36-37	30.69992513407113	36.5	25.0	38.0	11.0	38.0
38-39	29.02080457955396	34.0	22.5	37.5	11.0	38.0
40-41	30.16628894174208	35.0	25.0	37.5	11.0	38.0
42-43	31.197592576559323	36.5	28.0	38.0	11.0	38.0
44-45	32.773393056312415	37.0	33.0	38.0	11.0	38.0
46-47	31.374912783739322	37.0	28.5	38.0	11.0	38.0
48-49	30.801162204419633	36.0	27.0	38.0	11.0	38.0
50-51	28.684114162902148	34.0	21.5	37.5	11.0	38.0
52-53	31.960076597521784	36.5	29.0	38.0	11.0	38.0
54-55	31.037687610452707	36.5	27.0	38.0	11.0	38.0
56-57	31.452829371890132	36.5	28.0	38.0	11.0	38.0
58-59	32.528897229960634	37.0	32.0	38.0	11.0	38.0
60-61	32.97898151779212	38.0	34.0	38.0	11.0	38.0
62-63	32.88449511334294	38.0	33.5	38.0	11.0	38.0
64-65	32.60354161679849	37.5	32.5	38.0	11.0	38.0
66-67	30.538041568415743	36.5	25.0	38.0	11.0	38.0
68-69	30.63450911887385	36.5	25.0	38.0	11.0	38.0
70-71	30.208567819873593	35.5	24.0	38.0	11.0	38.0
72-73	29.003503929188035	34.0	22.5	37.5	11.0	38.0
74-75	31.096358052670986	36.0	28.0	38.0	11.0	38.0
76	31.4605731572541	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	0.0
5	1.0
6	0.0
7	1.0
8	1.0
9	2.0
10	1.0
11	11.0
12	65.0
13	326.0
14	976.0
15	1640.0
16	2698.0
17	4217.0
18	5439.0
19	5802.0
20	5161.0
21	4146.0
22	3399.0
23	3024.0
24	2948.0
25	3091.0
26	3325.0
27	3936.0
28	4697.0
29	5646.0
30	6802.0
31	8866.0
32	11197.0
33	14803.0
34	20199.0
35	26513.0
36	30558.0
37	16849.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.49508787834032	19.108127791556957	11.169283578896975	30.22750075120575
2	20.71850920036058	17.323059215384692	38.53201664366364	23.426414940591084
3	20.418640183346064	17.17799847211612	36.25261013496308	26.15075120957474
4	14.411220950663875	18.598196050869127	32.38755876076538	34.60302423770162
5	14.394120732769377	29.057656951072314	39.29595469338073	17.252267622777577
6	28.059953858141796	33.1727365788817	16.59833665221975	22.168972910756757
7	27.629092798101357	25.360706082474753	19.953043274544054	27.057157844879832
8	23.79366195031018	31.59194857847182	18.3611934521081	26.253196019109904
9	23.036803879024948	15.579256180668033	19.787305564893195	41.59663437541383
10-11	24.811545631424	23.216304868234744	26.788279156946835	25.18387034339442
12-13	24.37103835017101	19.478392853050007	28.860038047118326	27.290530749660658
14-15	24.053234321163085	22.397428943233532	23.304785842889487	30.244550892713896
16-17	25.233599637344362	22.597470069449574	25.651519511841066	26.517410781365
18-19	22.39237008646047	22.368940445417852	27.616670698941846	27.622018769179835
20-21	24.424586298048766	22.05722813326067	27.27606105828244	26.242124510408125
22-23	25.36449088422624	21.22412910553619	27.24112552238388	26.1702544878537
24-25	22.734854677739325	24.35398888150372	23.88363014064923	29.02752630010772
26-27	23.61355516111206	23.43758356104381	26.8689024312603	26.079958846583835
28-29	23.342653994641893	24.27981338304353	25.97102955108028	26.4065030712343
30-31	23.206863883625694	24.05109712120286	26.181671861948132	26.560367133223316
32-33	23.14879659363803	24.11167448055272	25.93963995854099	26.799888967268267
34-35	23.441327920870748	23.70770664724428	26.137202870574573	26.7137625613104
36-37	24.373448104618443	21.587358180637437	29.694268651394957	24.344925063349166
38-39	24.13533164402226	20.461971400557726	32.860071562273184	22.54262539314683
40-41	23.52202635318566	22.79876536292931	25.895035475442235	27.784172808442797
42-43	21.122086997267488	23.225076971266606	27.334285765217903	28.318550266248003
44-45	20.774292786781707	22.08048529546589	29.351513237646053	27.79370868010635
46-47	22.98647719459088	20.950671046935085	29.693635877454348	26.369215881019688
48-49	23.18210179160353	21.58302879044478	30.026358346172948	25.208511071778744
50-51	24.79391249207356	20.430485416618918	31.38336478443896	23.392237306868566
52-53	19.514903327017503	23.25499663834729	29.473799482509218	27.75630055212599
54-55	21.66228633131634	22.44412525721737	28.739583970009985	27.15400444145631
56-57	21.78091750408736	22.782257037644456	27.93946123247274	27.497364225795444
58-59	19.726688839879188	23.77873307085411	28.444785135458634	28.049792953808073
60-61	19.697147193798294	23.000718167603	28.244615016324303	29.057519622274402
62-63	19.584378541504847	23.53278239720882	28.25818445748921	28.62465460379713
64-65	20.38204719436479	24.00973835802727	27.013756958698565	28.594457488909374
66-67	19.443531853662886	24.98070825533866	23.252728221976035	32.32303166902242
68-69	19.807617354178184	24.86734207784466	23.662148357793	31.662892210184157
70-71	20.336168695563593	25.145163754902462	23.090459939897112	31.42820760963684
72-73	19.698470706581947	25.242127513274674	22.309602332777303	32.74979944736607
74-75	21.402868079059356	25.392762824479775	24.900233531210926	28.304135565249943
76	21.861550137268186	24.981281611156668	25.49164421308912	27.665524038486026
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	3.0
4	4.0
5	4.0
6	2.5
7	0.5
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	1.0
14	2.0
15	3.5
16	5.5
17	7.0
18	11.5
19	20.5
20	34.0
21	43.0
22	50.5
23	87.0
24	162.5
25	209.0
26	272.0
27	420.0
28	649.5
29	794.0
30	969.5
31	1342.0
32	1776.0
33	2013.0
34	2496.5
35	3340.5
36	4266.0
37	4831.0
38	5398.0
39	6635.5
40	7995.0
41	9154.0
42	9624.0
43	10154.5
44	11083.5
45	11673.5
46	11865.0
47	11746.5
48	11339.0
49	10548.5
50	10047.0
51	9549.0
52	8527.5
53	7427.0
54	6850.0
55	6507.0
56	5713.0
57	4958.0
58	4654.0
59	4365.0
60	3970.5
61	3718.5
62	3572.0
63	3413.0
64	3202.5
65	3017.0
66	2801.5
67	2720.0
68	2657.5
69	2444.5
70	2248.5
71	2203.0
72	2091.5
73	1821.5
74	1546.5
75	1430.0
76	1284.5
77	1018.5
78	782.5
79	667.0
80	570.5
81	395.0
82	264.5
83	213.0
84	181.0
85	107.0
86	51.5
87	38.0
88	29.0
89	14.0
90	8.5
91	6.5
92	4.0
93	3.0
94	1.0
95	0.5
96	1.0
97	1.5
98	1.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	5.092920331447255E-4
4	0.002037168132578902
5	0.0
6	0.0
7	0.0
8	0.006620796430881431
9	0.006620796430881431
10-11	0.008657964563460335
12-13	0.007894026513743244
14-15	0.0058568583811643435
16-17	0.010949778712611598
18-19	0.009421902613177421
20-21	0.007130088464026157
22-23	0.007894026513743244
24-25	0.0058568583811643435
26-27	0.006366150414309069
28-29	0.006620796430881431
30-31	0.009676548629749785
32-33	0.006366150414309069
34-35	0.007130088464026157
36-37	0.009421902613177421
38-39	0.009421902613177421
40-41	0.009167256596605059
42-43	0.0058568583811643435
44-45	0.008657964563460335
46-47	0.008148672530315608
48-49	0.009421902613177421
50-51	0.00738473448059852
52-53	0.009676548629749785
54-55	0.009676548629749785
56-57	0.007130088464026157
58-59	0.009167256596605059
60-61	0.009167256596605059
62-63	0.009421902613177421
64-65	0.007130088464026157
66-67	0.01196836277890105
68-69	0.038451548502426776
70-71	0.010695132696039236
72-73	0.009421902613177421
74-75	0.008912610580032697
76	0.01018584066289451
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	196351.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.54968628155979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.92368805487338	83.21287113439436
2	4.916185990131486	8.80485826236148
3	1.1933597399934521	3.205949710123722
4	0.4094798949190963	1.4667518451444457
5	0.2224870814080681	0.9961824170896176
6	0.12582363449076944	0.676048019727243
7	0.08934370627816825	0.5600490607909254
8	0.038607967134693305	0.2765865075904445
9	0.032625422380439056	0.2629436705074528
>10	0.04839850839042633	0.5377593722703033
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0015278760994341764	0.0
2	0.0	0.0	0.0	0.0015278760994341764	0.0
3	0.0	0.0	0.0	0.0015278760994341764	0.0
4	0.0	0.0	0.0	0.002037168132578902	0.0
5	0.0	0.0	0.0	0.0025464601657236275	0.0
6	0.0	0.0	0.0	0.003055752198868353	0.0
7	0.0	0.0	0.0	0.0035650442320130787	0.0
8	0.0	0.0	0.0	0.0045836282983025296	0.0
9	0.0	0.0	0.0	0.008657964563460335	0.0
10	0.0	0.0	0.0	0.009676548629749785	5.092920331447255E-4
11	0.0	0.0	0.0	0.010695132696039236	5.092920331447255E-4
12	0.0	0.0	0.0	0.011713716762328687	5.092920331447255E-4
13	0.0	0.0	0.0	0.013241592861762864	5.092920331447255E-4
14	0.0	0.0	0.0	0.013750884894907589	5.092920331447255E-4
15	0.0	0.0	5.092920331447255E-4	0.013750884894907589	5.092920331447255E-4
16	0.0	0.0	5.092920331447255E-4	0.013750884894907589	5.092920331447255E-4
17	0.0	0.0	5.092920331447255E-4	0.014260176928052315	5.092920331447255E-4
18	0.0	0.0	0.001018584066289451	0.014260176928052315	5.092920331447255E-4
19	0.0	0.0	0.001018584066289451	0.01731592912692067	5.092920331447255E-4
20	0.0	0.0	0.001018584066289451	0.02240884945836792	5.092920331447255E-4
21	0.0	0.0	0.001018584066289451	0.025464601657236276	5.092920331447255E-4
22	0.0	0.0	0.001018584066289451	0.03463185825384134	5.092920331447255E-4
23	0.0	0.0	0.001018584066289451	0.03717831841956496	5.092920331447255E-4
24	0.0	0.0	0.001018584066289451	0.042780530784156945	5.092920331447255E-4
25	0.0	0.0	0.001018584066289451	0.04532699094988057	5.092920331447255E-4
26	0.0	0.0	0.001018584066289451	0.04838274314874892	5.092920331447255E-4
27	0.0	0.0	0.001018584066289451	0.09014468986661642	5.092920331447255E-4
28	0.0	0.0	0.001018584066289451	0.14311106131366788	5.092920331447255E-4
29	0.0	0.0	0.001018584066289451	0.16857566297090415	5.092920331447255E-4
30	0.0	0.0	0.001018584066289451	0.22256061848424505	5.092920331447255E-4
31	0.0	0.0	0.001018584066289451	0.2495530962409155	5.092920331447255E-4
32	0.0	0.0	0.001018584066289451	0.30557521988683534	5.092920331447255E-4
33	0.0	0.0	0.001018584066289451	0.36261592759904454	5.092920331447255E-4
34	0.0	0.0	0.001018584066289451	0.4374818564713192	5.092920331447255E-4
35	0.0	0.0	0.001018584066289451	0.5902694664147369	5.092920331447255E-4
36	0.0	0.0	0.001018584066289451	0.7206482268997866	5.092920331447255E-4
37	0.0	0.0	0.001018584066289451	1.1275725613824223	5.092920331447255E-4
38	0.0	0.0	0.001018584066289451	1.5187088428375715	5.092920331447255E-4
39	0.0	0.0	0.001018584066289451	2.0137406990542446	5.092920331447255E-4
40	0.0	0.0	0.001018584066289451	2.631002643225652	5.092920331447255E-4
41	0.0	0.0	0.001018584066289451	3.318037595937887	5.092920331447255E-4
42	0.0	0.0	0.0015278760994341764	3.9923402478215033	5.092920331447255E-4
43	0.0	0.0	0.0015278760994341764	4.771557058532934	5.092920331447255E-4
44	0.0	0.0	0.0015278760994341764	5.569617674470718	5.092920331447255E-4
45	0.0	0.0	0.0015278760994341764	6.111504397736706	5.092920331447255E-4
46	0.0	0.0	0.0015278760994341764	6.796502182316362	5.092920331447255E-4
47	0.0	0.0	0.0015278760994341764	7.63479686887258	5.092920331447255E-4
48	0.0	0.0	0.0015278760994341764	8.139505273719003	5.092920331447255E-4
49	0.0	0.0	0.0015278760994341764	8.624860581305926	5.092920331447255E-4
50	0.0	0.0	0.0015278760994341764	9.25791057850482	5.092920331447255E-4
51	0.0	0.0	0.0015278760994341764	10.09213092879588	5.092920331447255E-4
52	0.0	0.0	0.0015278760994341764	10.759303492215471	5.092920331447255E-4
53	0.0	0.0	0.0015278760994341764	11.56856853288244	5.092920331447255E-4
54	0.0	0.0	0.0015278760994341764	12.382417201847712	5.092920331447255E-4
55	0.0	0.0	0.0015278760994341764	12.876430473998095	5.092920331447255E-4
56	0.0	0.0	0.0015278760994341764	13.44174463078874	5.092920331447255E-4
57	0.0	0.0	0.0015278760994341764	13.86853135456402	5.092920331447255E-4
58	0.0	0.0	0.0015278760994341764	14.284622945643262	5.092920331447255E-4
59	0.0	0.0	0.0015278760994341764	14.738911439208357	5.092920331447255E-4
60	0.0	0.0	0.0015278760994341764	15.0582375439901	5.092920331447255E-4
61	0.0	0.0	0.0015278760994341764	15.381128693003856	5.092920331447255E-4
62	0.0	0.0	0.0015278760994341764	15.632209665344204	5.092920331447255E-4
63	0.0	0.0	0.0015278760994341764	15.964268070954565	5.092920331447255E-4
64	0.0	0.0	0.0015278760994341764	16.294798600465494	5.092920331447255E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAC	20	7.915274E-5	69.99466	35
TTCTACG	20	0.006945595	52.49599	2
GCAATTG	20	0.006945595	52.49599	43
GATCTAT	20	0.006945595	52.49599	41
CCGTATC	130	0.0	48.457836	55
CTCGGTG	260	0.0	47.111786	44
TCGCCGT	195	0.0	46.6631	52
ACTCGGT	30	5.8696454E-4	46.6631	43
CATCGGA	60	4.9076334E-9	46.6631	9
CTCTCGG	70	3.8198777E-10	44.99656	42
GCCGTAT	140	0.0	44.99656	54
TGGTCGC	220	0.0	44.542053	49
TCGGTGG	205	0.0	44.386852	45
AGATCTC	150	0.0	44.32995	40
TCTCGGT	240	0.0	43.746655	43
ATCGGAC	65	9.958967E-9	43.07363	10
TAGATCT	140	0.0	42.49675	39
ATCTCGG	215	0.0	42.32235	42
GGTGGTC	210	0.0	41.663486	47
CGGTGGT	205	0.0	40.972477	46
>>END_MODULE