FastQCFastQC Report
Wed 25 May 2016
SRR1779710_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779710_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196351
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2299211.70964242606353No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG28361.4443522059984415No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC17100.8708893766774806No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT8680.44206548476962176Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC6950.35395796303558424No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC5240.2668690253678362Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC3090.15737123824172017Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGT150.002222150370.054
CCTTTGG207.912281E-570.09
ACGAAGA252.3861405E-456.00000441
TCTTTAT1050.053.3333321
TTAATAC407.4523086E-752.53
GTCTCAC200.00694349852.51
TATGCCG34050.050.05873543
GTATGCC34100.049.98533642
TCGTATG33900.049.970540
CGTATGC34050.049.85315341
CTCGTAT33850.049.83751739
CCGTCTT34100.049.7800647
TCTTCTG34050.049.75036650
TCTCGTA33500.049.73134638
GCCGTCT34150.049.70717246
GTCTTCT33950.049.69072349
CGTCTTC34150.049.60468348
CTGCTTG33950.049.5876354
ATGCCGT34350.049.5196544
CTTCTGC34100.049.36950351
Produced by FastQC (version 0.11.5)