##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779710_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	196351
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.636355302494	34.0	34.0	34.0	34.0	34.0
2	33.65235216525508	34.0	34.0	34.0	34.0	34.0
3	33.672107603220766	34.0	34.0	34.0	34.0	34.0
4	33.71213795702594	34.0	34.0	34.0	34.0	34.0
5	33.7096373331432	34.0	34.0	34.0	34.0	34.0
6	37.364449378918366	38.0	38.0	38.0	37.0	38.0
7	37.33788979938987	38.0	38.0	38.0	37.0	38.0
8	37.22491864059771	38.0	38.0	38.0	36.0	38.0
9	37.23724350779981	38.0	38.0	38.0	36.0	38.0
10-11	37.155754236036486	38.0	38.0	38.0	36.0	38.0
12-13	37.1267118578464	38.0	38.0	38.0	36.0	38.0
14-15	37.14748078695805	38.0	38.0	38.0	36.0	38.0
16-17	37.12859114544871	38.0	38.0	38.0	36.0	38.0
18-19	37.137019928597255	38.0	38.0	38.0	36.0	38.0
20-21	37.14802063651318	38.0	38.0	38.0	36.0	38.0
22-23	37.09629948408717	38.0	38.0	38.0	36.0	38.0
24-25	37.09570615886855	38.0	38.0	38.0	36.0	38.0
26-27	36.996875493376656	38.0	38.0	38.0	35.5	38.0
28-29	36.9389766285886	38.0	38.0	38.0	35.0	38.0
30-31	36.85165596304577	38.0	37.0	38.0	35.0	38.0
32-33	36.8647218501561	38.0	37.0	38.0	35.0	38.0
34-35	36.639166594516965	38.0	37.0	38.0	34.0	38.0
36-37	36.73323028657863	38.0	37.0	38.0	34.5	38.0
38-39	36.65568548161201	38.0	37.0	38.0	34.0	38.0
40-41	36.85963147628482	38.0	37.0	38.0	35.0	38.0
42-43	36.94027786973328	38.0	37.5	38.0	35.0	38.0
44-45	36.91235084109579	38.0	38.0	38.0	35.0	38.0
46-47	36.940667478138636	38.0	38.0	38.0	35.0	38.0
48-49	36.914418566750356	38.0	38.0	38.0	35.0	38.0
50-51	36.85387647631028	38.0	37.5	38.0	35.0	38.0
52-53	36.84474487015599	38.0	38.0	38.0	35.0	38.0
54-55	36.83954499849759	38.0	37.0	38.0	35.0	38.0
56-57	36.85235369313118	38.0	37.0	38.0	35.0	38.0
58-59	36.79241002083005	38.0	37.0	38.0	35.0	38.0
60-61	36.84363206706358	38.0	37.0	38.0	35.0	38.0
62-63	36.85825129487499	38.0	38.0	38.0	35.0	38.0
64-65	36.887102688552645	38.0	38.0	38.0	35.0	38.0
66-67	36.82624229059185	38.0	38.0	38.0	35.0	38.0
68-69	36.677101211605745	38.0	37.0	38.0	34.0	38.0
70-71	35.673650758081195	38.0	37.0	38.0	29.0	38.0
72-73	33.96216469485768	38.0	36.0	38.0	21.5	38.0
74-75	33.76462559396183	38.0	36.0	38.0	19.0	38.0
76	33.36105749397762	38.0	34.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	9.0
22	16.0
23	37.0
24	69.0
25	123.0
26	243.0
27	378.0
28	660.0
29	1029.0
30	1776.0
31	2641.0
32	4228.0
33	6594.0
34	11242.0
35	22805.0
36	32376.0
37	112124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.48822771465386	15.932691964899593	11.069462340400609	36.50961798004594
2	18.921726907425988	16.01519727426904	40.541173714419585	24.52190210388539
3	17.979536646108247	18.235710538780044	37.15692815417289	26.62782466093883
4	13.424937993694966	25.364780418739908	32.10729764554293	29.102983942022192
5	13.378592418678794	30.664473315643924	41.516467957891734	14.440466307785545
6	27.974392796573483	41.96973786739054	15.539009223278722	14.516860112757257
7	26.45568395373591	25.236438826387435	18.550962307296626	29.756914912580022
8	23.109635295975064	40.765771501036404	15.853242407729015	20.27135079525951
9	21.735565390550597	15.331218073755673	16.65996098823026	46.27325554746347
10-11	23.486002108469016	27.205871118558093	27.102994127862857	22.20513264511003
12-13	22.7177350764702	18.681086421765105	28.1391487692958	30.462029732468892
14-15	21.461820922735306	18.78498199652663	19.776318939042838	39.976878141695224
16-17	28.85343084578128	27.389216250490193	19.647977346690364	24.109375557038163
18-19	28.901558942913457	22.30062490132467	20.383140396534777	28.414675759227098
20-21	21.69940565619732	21.278730436819774	20.29859791903275	36.72326598795015
22-23	22.01414813268076	27.9244821773253	20.37015344968959	29.691216240304353
24-25	28.538179077264697	28.089747442080764	20.204888184934124	23.16718529572042
26-27	28.571792351452245	29.181669561143053	20.685914510239318	21.560623577165384
28-29	21.87485676161568	28.89341027038314	21.582268488574034	27.64946447942715
30-31	28.32325783927762	22.432022245876006	27.230062490132468	22.014657424713903
32-33	27.617888373372175	28.44650651129864	22.579462289471405	21.356142825857773
34-35	22.780377996547	28.312053414548437	28.029141690136537	20.878426898768023
36-37	21.117030216296325	27.445238374136117	29.836873761783743	21.600857647783815
38-39	20.4557145112579	22.106075344663385	29.588593895625692	27.849616248453025
40-41	20.524978227765583	19.848638407749387	30.20280008759823	29.4235832768868
42-43	27.44396514405325	19.707309868551725	29.455414028958344	23.39331095843668
44-45	20.043187964410674	26.693268687197925	30.811404067206176	22.452139281185225
46-47	26.466124440415378	20.008810752173403	25.02941161491411	28.495653192497112
48-49	25.8992824075253	19.276703454527862	24.10199082255756	30.72202331538928
50-51	19.718768939297483	19.752382213485035	31.014102296397777	29.514746550819705
52-53	17.993542177019727	19.96322911520695	38.19002704340696	23.853201664366363
54-55	17.31720235700353	20.245122255552555	32.45616268824707	29.981512699196845
56-57	24.35383573294763	20.31336738799395	25.168957631995763	30.163839247062658
58-59	18.388243502706885	20.642115395388867	36.20378811414253	24.765852987761715
60-61	24.496692148244726	27.236683286563345	24.38744900713518	23.879175558056744
62-63	17.852722929855208	36.11950028265708	21.766886850588996	24.260889936898717
64-65	17.656900143111063	37.155909570106594	20.545604555107943	24.641585731674397
66-67	17.738132222397645	37.189777490310725	19.995314513295067	25.07677577399657
68-69	17.63500058568584	37.70365315175375	19.830049248539606	24.83129701402081
70-71	18.268559874917877	36.333657582594434	19.928087964919968	25.469694577567726
72-73	18.727177350764702	32.88091224388977	20.962714730253474	27.429195675092053
74-75	19.415994825592943	31.892121761539283	21.62912335562335	27.06276005724442
76	19.748307877219876	32.196933043376404	21.550183090485916	26.504575988917807
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	1.0
15	0.0
16	9.5
17	19.0
18	14.5
19	17.5
20	37.0
21	49.0
22	60.5
23	99.0
24	214.5
25	303.0
26	331.5
27	427.0
28	630.5
29	767.0
30	930.5
31	1183.5
32	1662.0
33	2051.0
34	2261.0
35	2878.0
36	3731.5
37	4178.0
38	9215.0
39	15393.5
40	15515.5
41	13106.5
42	11717.0
43	10781.0
44	10091.5
45	9904.5
46	9471.0
47	9473.5
48	9136.5
49	8527.0
50	8257.0
51	7638.0
52	6503.5
53	5516.0
54	5044.0
55	4698.5
56	4106.0
57	3518.0
58	3177.0
59	3078.0
60	2912.5
61	2795.0
62	2744.0
63	2680.0
64	2647.5
65	2467.5
66	2396.5
67	2537.0
68	2460.5
69	2320.0
70	2239.0
71	2222.0
72	2147.0
73	1995.5
74	1725.0
75	1531.0
76	1524.0
77	1416.0
78	1184.5
79	1054.0
80	906.0
81	633.5
82	448.0
83	387.0
84	321.5
85	195.5
86	118.0
87	101.0
88	68.0
89	29.5
90	16.5
91	7.5
92	6.0
93	3.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	196351.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.24251977326319
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.3556932105306	13.85783622186798
2	23.791670062759803	14.86623444749454
3	13.575678539408266	12.724152156087824
4	6.83674301084033	8.543883148035915
5	3.384138886624827	5.2864513040422505
6	1.8811639090390415	3.52633803749408
7	1.2079224060640639	2.641697775921691
8	0.826473225201728	2.0656884864350067
9	0.6210775124297009	1.7463623816532639
>10	3.447713750101883	17.99328753100315
>50	0.05053386584073682	0.9951566327647937
>100	0.011410872931779281	0.6651353952870115
>500	0.004890374113619692	1.062892473173042
>1k	0.003260249409079794	2.315241582675922
>5k	0.0	0.0
>10k+	0.001630124704539897	11.70964242606353
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT	22992	11.70964242606353	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	2836	1.4443522059984415	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	1710	0.8708893766774806	No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT	868	0.44206548476962176	Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	695	0.35395796303558424	No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	524	0.2668690253678362	Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	309	0.15737123824172017	Illumina PCR Primer Index 10 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0015278760994341764	0.0
2	0.0	0.0	0.0	0.002037168132578902	0.0
3	0.0	0.0	0.0	0.002037168132578902	0.0
4	0.0	0.0	0.0	0.003055752198868353	0.0
5	0.0	0.0	0.0	0.0045836282983025296	0.0
6	0.0	0.0	0.0	0.005092920331447255	0.0
7	0.0	0.0	0.0	0.00560221236459198	0.0
8	0.0	0.0	0.0	0.006620796430881432	0.0
9	0.0	0.0	0.0	0.01120442472918396	0.0
10	0.0	0.0	0.0	0.012732300828618138	0.0
11	0.0	0.0	0.0	0.014260176928052315	0.0
12	0.0	0.0	0.0	0.016297345060631217	0.0
13	0.0	0.0	0.0	0.017825221160065394	0.0
14	0.0	0.0	0.0	0.018843805226354843	0.0
15	0.0	0.0	0.0	0.02037168132578902	0.0
16	0.0	0.0	0.0	0.020880973358933748	0.0
17	0.0	0.0	0.0	0.02240884945836792	0.0
18	0.0	0.0	0.0	0.02291814149151265	0.0
19	0.0	0.0	0.0	0.026992477756670453	0.0
20	0.0	0.0	0.0	0.03412256622069661	0.0
21	0.0	0.0	0.0	0.040234070618433315	0.0
22	0.0	0.0	0.0	0.0458362829830253	0.0
23	0.0	0.0	0.0	0.052457079413906725	0.0
24	0.0	0.0	0.0	0.055003539579630355	0.0
25	0.0	0.0	0.0	0.05856858381164343	0.0
26	0.0	0.0	0.0	0.06162433601051179	0.0
27	0.0	0.0	0.0	0.11153495525869489	0.0
28	0.0	0.0	0.0	0.18487300803153536	0.0
29	0.0	0.0	0.0	0.2434415918431788	0.0
30	0.0	0.0	0.0	0.32085398088117706	0.0
31	0.0	0.0	0.0	0.4033592902506226	0.0
32	0.0	0.0	0.0	0.4965597323161074	0.0
33	0.0	0.0	0.0	0.6269384928011571	0.0
34	0.0	0.0	0.0	0.8026442442360874	0.0
35	0.0	0.0	0.0	1.024186278654043	0.0
36	0.0	0.0	0.0	1.376616365590193	0.0
37	0.0	0.0	0.0	1.9750345045352455	0.0
38	0.0	0.0	0.0	2.6376234396565335	0.0
39	0.0	0.0	0.0	3.36794821518607	0.0
40	0.0	0.0	0.0	4.262774317421353	0.0
41	0.0	0.0	0.0	5.1973251982419235	0.0
42	0.0	0.0	0.0	6.180768114244389	0.0
43	0.0	0.0	0.0	7.224816782191076	0.0
44	0.0	0.0	0.0	8.293820759761855	0.0
45	0.0	0.0	0.0	9.37097340986295	0.0
46	0.0	0.0	0.0	10.46493269705782	0.0
47	0.0	0.0	0.0	11.618988444163769	0.0
48	0.0	0.0	0.0	12.54182560822201	0.0
49	0.0	0.0	0.0	13.481978701407174	0.0
50	0.0	0.0	0.0	14.279530025311814	0.0
51	0.0	0.0	0.0	15.256352144883397	0.0
52	0.0	0.0	0.0	16.19548665400227	0.0
53	0.0	0.0	0.0	17.077580455408935	0.0
54	0.0	0.0	0.0	17.98972248677114	0.0
55	0.0	0.0	0.0	18.841768058222264	0.0
56	0.0	0.0	0.0	19.65816318735326	0.0
57	0.0	0.0	0.0	20.403766723877137	0.0
58	0.0	0.0	0.0	21.1605746851302	0.0
59	0.0	0.0	0.0	21.825710080417213	0.0
60	0.0	0.0	0.0	22.54890476748272	0.0
61	0.0	0.0	0.0	23.254783525421313	0.0
62	0.0	0.0	0.0	23.975941044354244	0.0
63	0.0	0.0	0.0	24.657373784701885	0.0
64	0.0	0.0	0.0	25.386679976165134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTCGT	15	0.0022221503	70.0	54
CCTTTGG	20	7.912281E-5	70.0	9
ACGAAGA	25	2.3861405E-4	56.000004	41
TCTTTAT	105	0.0	53.333332	1
TTAATAC	40	7.4523086E-7	52.5	3
GTCTCAC	20	0.006943498	52.5	1
TATGCCG	3405	0.0	50.058735	43
GTATGCC	3410	0.0	49.985336	42
TCGTATG	3390	0.0	49.9705	40
CGTATGC	3405	0.0	49.853153	41
CTCGTAT	3385	0.0	49.837517	39
CCGTCTT	3410	0.0	49.78006	47
TCTTCTG	3405	0.0	49.750366	50
TCTCGTA	3350	0.0	49.731346	38
GCCGTCT	3415	0.0	49.707172	46
GTCTTCT	3395	0.0	49.690723	49
CGTCTTC	3415	0.0	49.604683	48
CTGCTTG	3395	0.0	49.58763	54
ATGCCGT	3435	0.0	49.51965	44
CTTCTGC	3410	0.0	49.369503	51
>>END_MODULE