FastQCFastQC Report
Wed 25 May 2016
SRR1779709_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779709_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172818
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTT200.00693970852.50456254
CAGTGTT200.00693970852.50456234
TCTCGGT1600.052.4893743
CGACTAG200.00694765752.4893722
ATCTCGG1500.051.32293742
TCGGTGG1500.051.32293745
GGTGGTC1300.048.4517247
ATCGTAC305.862955E-446.6707210
CTCGGTG1750.045.99068544
GTAGATC950.044.21436338
CTTCCGA1200.043.753818
TCGTTGG404.584014E-543.7411445
CGGTGGT1450.043.43947646
CCGTATC1500.042.00364755
CTAACGC503.4616132E-642.00364712
TCGCCGT1350.041.47308352
GACGCTT602.5809823E-740.8368814
GTATCAT1050.040.0034757
TGTGTCG350.001252274840.0034735
CGTATCA1150.039.5686556