##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779709_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172818
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.112580865419112	34.0	23.0	34.0	12.0	34.0
2	27.706083857005638	34.0	23.0	34.0	12.0	34.0
3	26.653595111620316	32.0	21.0	34.0	11.0	34.0
4	28.74828432223495	33.0	23.0	34.0	12.0	34.0
5	28.464477079933804	34.0	23.0	34.0	11.0	34.0
6	30.70825955629622	36.0	26.0	38.0	11.0	38.0
7	31.721145945445496	37.0	27.0	38.0	12.0	38.0
8	30.93119350993531	37.0	26.0	38.0	11.0	38.0
9	32.347446446550705	37.0	31.0	38.0	12.0	38.0
10-11	31.986448170908123	37.0	31.0	38.0	11.0	38.0
12-13	31.13629945954704	36.0	27.0	38.0	11.0	38.0
14-15	31.040661273709915	36.5	26.5	38.0	11.0	38.0
16-17	30.78284380099295	36.0	27.0	38.0	11.0	38.0
18-19	31.337823606337302	37.0	27.0	38.0	11.5	38.0
20-21	30.27336851485378	35.5	24.5	38.0	11.0	38.0
22-23	30.246866645835503	35.5	26.0	38.0	11.0	38.0
24-25	31.34918527005289	36.5	28.5	38.0	11.0	38.0
26-27	30.0050052656552	35.0	24.0	38.0	11.0	38.0
28-29	31.953691166429422	37.0	29.5	38.0	11.0	38.0
30-31	32.42250228564154	37.0	31.0	38.0	11.0	38.0
32-33	32.51438218241155	38.0	31.0	38.0	11.0	38.0
34-35	32.57115867560092	38.0	32.0	38.0	11.0	38.0
36-37	30.608377020912172	36.0	25.0	38.0	11.0	38.0
38-39	29.019873508546564	34.0	22.5	37.5	11.0	38.0
40-41	30.046094735502088	35.0	24.5	37.5	11.0	38.0
42-43	31.058561608165817	36.5	27.5	38.0	11.0	38.0
44-45	32.60284518973718	37.0	31.5	38.0	11.0	38.0
46-47	31.282762790912983	37.0	28.5	38.0	11.0	38.0
48-49	30.714312166556724	36.0	25.0	38.0	11.0	38.0
50-51	28.649266858776286	34.0	21.5	37.5	11.0	38.0
52-53	31.779872466988394	36.5	29.0	38.0	11.0	38.0
54-55	30.892650071173144	36.5	27.0	38.0	11.0	38.0
56-57	31.31629807080281	36.5	27.5	38.0	11.0	38.0
58-59	32.33623523012649	37.0	31.0	38.0	11.0	38.0
60-61	32.793331713131735	38.0	34.0	38.0	11.0	38.0
62-63	32.677982038907984	38.0	33.0	38.0	11.0	38.0
64-65	32.41846335451168	37.5	31.5	38.0	11.0	38.0
66-67	30.463918689025448	36.5	24.5	38.0	11.0	38.0
68-69	30.540273582612922	36.5	25.0	38.0	11.0	38.0
70-71	30.119440683262162	35.5	24.0	38.0	11.0	38.0
72-73	28.954964181971786	34.0	22.5	37.5	11.0	38.0
74-75	30.973978405027253	36.0	26.0	38.0	11.0	38.0
76	31.345548496105728	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	2.0
5	0.0
6	2.0
7	0.0
8	1.0
9	0.0
10	0.0
11	15.0
12	66.0
13	362.0
14	867.0
15	1586.0
16	2576.0
17	4034.0
18	5190.0
19	5437.0
20	5019.0
21	3857.0
22	3083.0
23	2598.0
24	2540.0
25	2512.0
26	2910.0
27	3219.0
28	3808.0
29	4654.0
30	5655.0
31	7204.0
32	9402.0
33	12656.0
34	17366.0
35	23345.0
36	27278.0
37	15573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.43328819914592	17.764353250240138	11.425314492703306	30.377044057910634
2	21.240842967746413	16.125635061162612	38.30850953025727	24.32501244083371
3	19.913434943119352	16.890023030008447	36.99788216505225	26.19865986181995
4	14.551724936059902	19.469036790149175	32.51976067307803	33.45947760071289
5	14.375817333842539	28.70765776713074	39.278894559594484	17.637630339432235
6	27.349581640801308	32.613500908470186	17.145204781909293	22.89171266881922
7	27.15862930944693	25.553472439213508	20.185397354442244	27.102500896897315
8	23.352718224691145	31.53140641726702	17.47128432138414	27.644591036657694
9	22.846975088967973	15.83774556606776	19.202036860226254	42.11324248473802
10-11	24.613158960708294	23.177767490307275	26.9284184943001	25.28065505468434
12-13	24.411640462702028	19.24617067200583	28.88820734791188	27.45398151738026
14-15	23.683045595396194	22.139474057292976	23.598561454978924	30.578918892331906
16-17	25.171504873892196	22.499182621225227	25.72587575263948	26.60343675224309
18-19	22.154839680344423	22.390067761890155	27.77282696124668	27.68226559651874
20-21	24.688971958290416	21.959447729929288	26.94399759278762	26.40758271899267
22-23	25.471037555697006	21.08037729298073	27.045020542792663	26.4035646085296
24-25	22.4382862499566	24.524922749314293	23.882903005543533	29.153887995185574
26-27	23.57527869478545	23.397920892054774	27.041133701165123	25.985666711994654
28-29	23.27762796995614	24.399064890575993	26.012360109713335	26.310947029754534
30-31	22.94552156540508	23.937064373609395	26.33505293339853	26.782361127586995
32-33	22.955448953493082	24.00542783239687	26.226904225955224	26.81221898815483
34-35	23.247006130957722	23.645417116337743	26.188361306973206	26.919215445731332
36-37	24.50219606617711	21.296633855875562	30.31815472573766	23.883015352209664
38-39	24.14992361464747	20.126672376278876	33.30488171843896	22.418522290634694
40-41	23.444380790352355	23.035837253846733	25.54294047185042	27.976841483950487
42-43	20.851838242740047	23.550396523439872	27.30760439662181	28.29016083719827
44-45	20.695332143198144	22.13072776670264	29.520078467908306	27.653861622190913
46-47	23.34517657815095	21.07248875952619	29.534815089142597	26.04751957318026
48-49	23.216471221366756	21.402345343598913	30.483680100456862	24.89750333457747
50-51	25.311537070524416	20.366003616636526	31.24658227848101	23.075877034358047
52-53	19.703891880318388	23.168420534869526	29.688065112537863	27.439622472274223
54-55	21.90147446877459	22.232767001527705	28.69774547474654	27.168013054951164
56-57	21.94481896616574	22.69186924594793	28.00483759902322	27.35847418886311
58-59	19.73340817424817	23.81185225477545	28.67641527449063	27.778324296485756
60-61	19.710893014212306	23.15199527799639	28.286306189528265	28.850805518263044
62-63	19.343153338483486	23.69676611085553	28.21153807205002	28.74854247861097
64-65	20.294018933870333	24.165277874221697	27.217438603800666	28.323264588107307
66-67	19.046337687889704	25.2130260550034	23.572761598888935	32.16787465821796
68-69	19.53435876658612	25.65888668624926	23.573485363700822	31.2332691834638
70-71	19.89879028641365	25.691585242709458	23.07569896330931	31.333925507567585
72-73	19.389278522324837	25.6000740689304	22.29301205934773	32.717635349397035
74-75	20.99883109969215	25.63103256718283	25.061049001226763	28.309087331898247
76	21.810080435160003	24.528094438979224	25.501996412244658	28.159828713616108
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.5
10	2.0
11	1.5
12	0.0
13	0.0
14	1.0
15	5.0
16	9.0
17	10.0
18	15.5
19	24.5
20	39.0
21	50.0
22	59.5
23	103.0
24	157.5
25	178.0
26	238.5
27	379.0
28	599.5
29	740.0
30	844.0
31	1163.5
32	1632.5
33	1886.0
34	2194.5
35	2893.5
36	3749.0
37	4214.0
38	4800.5
39	5999.0
40	7191.5
41	8249.5
42	8727.0
43	9096.0
44	9770.5
45	10139.0
46	10202.0
47	10244.5
48	10120.5
49	9465.5
50	8977.0
51	8460.0
52	7417.0
53	6411.5
54	5932.0
55	5631.0
56	4860.0
57	4164.0
58	3938.0
59	3776.0
60	3431.0
61	3140.0
62	3032.0
63	2962.5
64	2786.0
65	2545.5
66	2408.5
67	2405.0
68	2308.5
69	2150.0
70	1978.0
71	1868.0
72	1779.5
73	1595.0
74	1426.5
75	1354.0
76	1222.5
77	958.5
78	720.5
79	615.0
80	524.5
81	363.0
82	238.5
83	185.0
84	157.0
85	97.0
86	51.5
87	38.0
88	29.0
89	14.0
90	8.0
91	5.0
92	2.0
93	1.5
94	1.5
95	1.0
96	0.0
97	0.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0017359302850397528
9	0.0017359302850397528
10-11	0.004629147426772675
12-13	0.00405050399842609
14-15	0.002603895427559629
16-17	0.00549711256929255
18-19	0.00405050399842609
20-21	0.0023145737133863373
22-23	0.004629147426772675
24-25	0.0023145737133863373
26-27	0.002025251999213045
28-29	0.0023145737133863373
30-31	0.0037611822842527976
32-33	0.0028932171417329214
34-35	0.0037611822842527976
36-37	0.005207790855119258
38-39	0.005786434283465843
40-41	0.005207790855119258
42-43	0.002025251999213045
44-45	0.004918469140945967
46-47	0.0028932171417329214
48-49	0.0043398257125993826
50-51	0.003182538855906214
52-53	0.00549711256929255
54-55	0.005786434283465843
56-57	0.0028932171417329214
58-59	0.00405050399842609
60-61	0.005786434283465843
62-63	0.0043398257125993826
64-65	0.0034718605700795055
66-67	0.006075755997639135
68-69	0.026328275989769587
70-71	0.004918469140945967
72-73	0.0034718605700795055
74-75	0.0034718605700795055
76	0.004629147426772675
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	172818.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.14180198081407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.85733807857142	85.54326921603476
2	4.357680777947716	7.94333757118621
3	0.9926826852100288	2.714246661755857
4	0.38908146783104786	1.4184634438184736
5	0.17946776177001905	0.8178507602591494
6	0.09266225890401496	0.5067243151274794
7	0.05466377445896753	0.34875084350841695
8	0.01985791001244445	0.14479085616856296
9	0.024812466945357867	0.20353076540903714
>10	0.03175281834897691	0.35903556673205417
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.786434283465842E-4	0.0
4	0.0	0.0	0.0	5.786434283465842E-4	0.0
5	0.0	0.0	0.0	5.786434283465842E-4	0.0
6	0.0	0.0	0.0	5.786434283465842E-4	0.0
7	0.0	0.0	0.0	5.786434283465842E-4	0.0
8	0.0	0.0	0.0	0.0017359302850397528	0.0
9	0.0	0.0	0.0	0.005786434283465843	0.0
10	0.0	0.0	0.0	0.006365077711812427	0.0
11	0.0	0.0	0.0	0.009258294853545347	0.0
12	0.0	0.0	0.0	0.011572868566931686	0.0
13	0.0	0.0	0.0	0.01215151199527827	0.0
14	0.0	0.0	0.0	0.01215151199527827	0.0
15	0.0	0.0	0.0	0.01215151199527827	0.0
16	0.0	0.0	0.0	0.01215151199527827	0.0
17	0.0	0.0	0.0	0.01215151199527827	0.0
18	0.0	0.0	0.0	0.014466085708664606	0.0
19	0.0	0.0	0.0	0.018516589707090695	0.0
20	0.0	0.0	0.0	0.019673876563783867	0.0
21	0.0	0.0	0.0	0.02025251999213045	0.0
22	0.0	0.0	0.0	0.02835352798898263	0.0
23	0.0	0.0	0.0	0.03008945827402238	0.0
24	0.0	0.0	0.0	0.034718605700795054	0.0
25	0.0	0.0	0.0	0.03529724912914164	0.0
26	0.0	0.0	0.0	0.04281961369764724	0.0
27	0.0	0.0	0.0	0.0810100799685218	0.0
28	0.0	0.0	0.0	0.1174646159543566	0.0
29	0.0	0.0	0.0	0.1469754308000324	0.0
30	0.0	0.0	0.0	0.20368248677799766	0.0
31	0.0	0.0	0.0	0.22972144105359396	0.0
32	0.0	0.0	0.0	0.2794847758914002	0.0
33	0.0	0.0	0.0	0.3269335370158201	0.0
34	0.0	5.786434283465842E-4	0.0	0.3923202444189841	0.0
35	0.0	5.786434283465842E-4	0.0	0.5618627689245334	0.0
36	0.0	5.786434283465842E-4	0.0	0.6677545163119583	0.0
37	0.0	5.786434283465842E-4	0.0	1.009154139036443	0.0
38	0.0	5.786434283465842E-4	0.0	1.3158351560601327	0.0
39	0.0	5.786434283465842E-4	0.0	1.7781712553090534	0.0
40	0.0	5.786434283465842E-4	0.0	2.2763832471154624	0.0
41	0.0	5.786434283465842E-4	0.0	2.850976171463621	0.0
42	0.0	5.786434283465842E-4	0.0	3.4469789026606024	0.0
43	0.0	5.786434283465842E-4	0.0	4.05050399842609	0.0
44	0.0	5.786434283465842E-4	0.0	4.715365297596315	0.0
45	0.0	5.786434283465842E-4	0.0	5.1684431019916905	0.0
46	0.0	5.786434283465842E-4	0.0	5.727412653774491	0.0
47	0.0	5.786434283465842E-4	0.0	6.429885775787245	0.0
48	0.0	5.786434283465842E-4	0.0	6.896272379034591	0.0
49	0.0	5.786434283465842E-4	0.0	7.334884097721302	0.0
50	0.0	5.786434283465842E-4	0.0	7.945931558055295	0.0
51	0.0	5.786434283465842E-4	0.0	8.692381580622389	0.0
52	0.0	5.786434283465842E-4	0.0	9.271603652397319	0.0
53	0.0	5.786434283465842E-4	0.0	9.9694476269833	0.0
54	0.0	5.786434283465842E-4	0.0	10.685229547848024	0.0
55	0.0	5.786434283465842E-4	0.0	11.170132740802464	0.0
56	0.0	5.786434283465842E-4	0.0	11.673552523463991	0.0
57	0.0	5.786434283465842E-4	0.0	12.081496140448333	0.0
58	0.0	5.786434283465842E-4	0.0	12.499855339142913	0.0
59	0.0	5.786434283465842E-4	0.0	12.981286671527272	0.0
60	0.0	5.786434283465842E-4	0.0	13.294332766262773	0.0
61	0.0	5.786434283465842E-4	0.0	13.582497193579373	0.0
62	0.0	5.786434283465842E-4	0.0	13.805274913492807	0.0
63	0.0	5.786434283465842E-4	0.0	14.115427791086576	0.0
64	0.0	5.786434283465842E-4	0.0	14.427316598965385	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTTT	20	0.006939708	52.504562	54
CAGTGTT	20	0.006939708	52.504562	34
TCTCGGT	160	0.0	52.48937	43
CGACTAG	20	0.006947657	52.48937	22
ATCTCGG	150	0.0	51.322937	42
TCGGTGG	150	0.0	51.322937	45
GGTGGTC	130	0.0	48.45172	47
ATCGTAC	30	5.862955E-4	46.67072	10
CTCGGTG	175	0.0	45.990685	44
GTAGATC	95	0.0	44.214363	38
CTTCCGA	120	0.0	43.7538	18
TCGTTGG	40	4.584014E-5	43.74114	45
CGGTGGT	145	0.0	43.439476	46
CCGTATC	150	0.0	42.003647	55
CTAACGC	50	3.4616132E-6	42.003647	12
TCGCCGT	135	0.0	41.473083	52
GACGCTT	60	2.5809823E-7	40.83688	14
GTATCAT	105	0.0	40.00347	57
TGTGTCG	35	0.0012522748	40.00347	35
CGTATCA	115	0.0	39.56865	56
>>END_MODULE