FastQCFastQC Report
Wed 25 May 2016
SRR1779709_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779709_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172818
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT2196412.709324260204378No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG26241.5183603559814371No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC15840.9165711905009896No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT8880.5138353643717669RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC6880.39810667870245No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC5240.3032091564536102No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC3350.19384554849610575RNA PCR Primer, Index 24 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC2240.12961612794963487No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC1980.1145713988126237No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1960.11341411195593051No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT1940.11225682509923733No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGATT150.002221698270.022
GACGCGA207.909599E-570.026
CTTTATA1000.056.0000042
CGGAATC200.006942095752.520
AGGAGCA200.006942095752.560
GACGTCA200.006942095752.526
TTAGAAC200.006942095752.53
AATGGTC200.006942095752.527
TACATTG200.006942095752.55
GCGGAAT200.006942095752.519
CGTCTTC30000.052.0333348
TATGCCG30350.051.8945643
CGTATGC30500.051.8688541
TCGTATG30450.051.83907740
CCGTCTT30200.051.80463447
ATGCCGT30350.051.7792444
GTATGCC30500.051.75409742
TCTCGTA29950.051.65275238
ATCTCGT30000.051.5666637
TCTTCTG30350.051.548650
Produced by FastQC (version 0.11.5)