##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779709_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172818
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.58505479753266	34.0	34.0	34.0	34.0	34.0
2	33.6142647177956	34.0	34.0	34.0	34.0	34.0
3	33.636096934347115	34.0	34.0	34.0	34.0	34.0
4	33.67815273871935	34.0	34.0	34.0	34.0	34.0
5	33.673679825018226	34.0	34.0	34.0	34.0	34.0
6	37.314105012209374	38.0	38.0	38.0	36.0	38.0
7	37.290264902961496	38.0	38.0	38.0	36.0	38.0
8	37.15695702993901	38.0	38.0	38.0	36.0	38.0
9	37.16999386637966	38.0	38.0	38.0	36.0	38.0
10-11	37.08687463111481	38.0	38.0	38.0	36.0	38.0
12-13	37.05102767072874	38.0	38.0	38.0	36.0	38.0
14-15	37.08030992142022	38.0	38.0	38.0	36.0	38.0
16-17	37.039165480447636	38.0	38.0	38.0	35.5	38.0
18-19	37.03650950711153	38.0	38.0	38.0	35.0	38.0
20-21	37.054181277413235	38.0	38.0	38.0	35.5	38.0
22-23	37.01541795414829	38.0	38.0	38.0	35.0	38.0
24-25	36.97463805853557	38.0	38.0	38.0	35.0	38.0
26-27	36.86931627492507	38.0	37.5	38.0	34.5	38.0
28-29	36.815198648288955	38.0	37.0	38.0	34.5	38.0
30-31	36.661991227765625	38.0	37.0	38.0	34.0	38.0
32-33	36.710070710226944	38.0	37.0	38.0	34.0	38.0
34-35	36.68634632966473	38.0	37.0	38.0	34.0	38.0
36-37	36.74706627781828	38.0	37.0	38.0	34.5	38.0
38-39	36.39785786202826	38.0	37.0	38.0	34.0	38.0
40-41	36.67540128921756	38.0	37.0	38.0	34.0	38.0
42-43	36.82057713895543	38.0	37.0	38.0	35.0	38.0
44-45	36.792376951474964	38.0	37.0	38.0	34.5	38.0
46-47	36.805836197618305	38.0	37.0	38.0	34.5	38.0
48-49	36.802728882408076	38.0	37.0	38.0	34.5	38.0
50-51	36.73464569662882	38.0	37.0	38.0	34.0	38.0
52-53	36.71895578006921	38.0	37.0	38.0	34.0	38.0
54-55	36.69858463817427	38.0	37.0	38.0	34.0	38.0
56-57	36.72295420615908	38.0	37.0	38.0	34.0	38.0
58-59	36.65557696536241	38.0	37.0	38.0	34.0	38.0
60-61	36.696142762906646	38.0	37.0	38.0	34.0	38.0
62-63	36.72583006399796	38.0	37.0	38.0	34.0	38.0
64-65	36.752760129153216	38.0	37.0	38.0	34.5	38.0
66-67	36.69380793667327	38.0	37.0	38.0	34.0	38.0
68-69	36.526898239766695	38.0	37.0	38.0	34.0	38.0
70-71	35.423196657755554	38.0	36.5	38.0	28.5	38.0
72-73	33.57982386094041	38.0	34.5	38.0	20.0	38.0
74-75	33.362667661933365	38.0	35.0	38.0	14.5	38.0
76	32.930904188221135	38.0	34.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	6.0
21	4.0
22	20.0
23	34.0
24	67.0
25	126.0
26	226.0
27	418.0
28	640.0
29	1161.0
30	1726.0
31	2872.0
32	4168.0
33	6668.0
34	11036.0
35	21708.0
36	28857.0
37	93080.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.45453598583481	15.415639574581352	11.101852816257566	37.02797162332627
2	18.945364487495517	15.999490793783055	41.00614519320904	24.04899952551239
3	17.448413938362904	18.40259695170642	38.14706801374857	26.001921096182112
4	13.146778692034394	26.406971496024717	31.83811871448576	28.608131097455125
5	13.172239002881645	29.878832066104227	42.43539446122511	14.513534469789027
6	26.90518348783113	43.15117638208983	15.682394195049126	14.261245935029915
7	25.780300663125367	25.310442199307943	18.20759411635362	30.701663021213065
8	22.666620375192398	41.581316760985544	15.910379705817682	19.841683158004376
9	20.917381291300675	15.657512527630224	16.36461479706975	47.060491383999356
10-11	22.71233320603178	27.656841301253344	27.602738140702936	22.028087352011944
12-13	22.3362149776065	18.46943026768045	28.72183453112523	30.472520223587825
14-15	20.863856774178615	18.687578840167113	19.654781330648426	40.79378305500585
16-17	28.98367068245206	27.63687810297538	19.273165989653858	24.1062852249187
18-19	29.019835896723723	22.190107511948987	20.155597217882395	28.634459373444898
20-21	21.230138064322006	20.869064565033735	19.964066243099676	37.93673112754458
22-23	21.566908539619718	28.34224444212987	20.042761749354813	30.0480852688956
24-25	28.602344663171657	28.6885625339953	19.846890948859492	22.862201853973545
26-27	28.969493918457566	29.32304505317733	20.708491013667558	20.998970014697544
28-29	28.50455392378109	22.34315869874666	21.15259984492356	27.999687532548695
30-31	29.246085477207235	20.578296242289575	28.631566156303162	21.544052124200025
32-33	21.84321077665521	28.60147669802914	28.496452915784236	21.058859609531414
34-35	27.739876633221076	28.89311298591582	22.768461618581398	20.598548762281705
36-37	22.37672001759076	27.86602090060063	20.95123193185895	28.80602714994966
38-39	20.164276869307596	22.032716499438713	29.028226226434743	28.774780404818944
40-41	20.243551018991077	19.692971796919302	30.409158768183868	29.65431841590575
42-43	27.608524574986404	19.840525871147683	29.7859597958546	22.764989758011318
44-45	20.12782233332176	27.347845710516268	30.976807971391867	21.547523984770105
46-47	26.8383501718571	20.1891585367265	24.410651668229004	28.561839623187403
48-49	26.18679767153884	19.370378085616082	23.587242069691815	30.855582173153262
50-51	19.657963869504332	19.714381603768125	31.037854853082436	29.589799673645107
52-53	17.839576895925195	19.61977340323346	38.83825758890856	23.702392111932785
54-55	17.418324480088877	19.85788517399808	32.48041291995047	30.243377425962574
56-57	24.880799453760606	19.90706986540754	25.017648624564572	30.194482056267287
58-59	18.48968278767258	20.367380712657244	36.95101204735618	24.191924452313994
60-61	25.017937946278746	27.99679431540696	24.141293152333667	22.84397458598063
62-63	17.769271719381084	37.634968579661845	21.222326378039323	23.37343332291775
64-65	17.34541540811721	38.51595319931951	20.619669247416358	23.51896214514692
66-67	17.448124616648727	38.58510108900693	20.045365644782372	23.921408649561968
68-69	17.50888217662512	38.79138747121249	19.81304030830122	23.886690043861172
70-71	18.279056579754425	37.15787707299008	20.08644932819498	24.476617019060516
72-73	18.832818340682106	33.07612632943327	21.125692925505447	26.965362404379174
74-75	19.40885787360113	31.238354801004526	22.263016583920656	27.08977074147369
76	19.917485447117777	31.503084169473087	21.96530454003634	26.6141258433728
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	3.0
16	7.0
17	9.0
18	8.5
19	18.0
20	50.5
21	73.0
22	69.5
23	113.0
24	195.5
25	231.0
26	315.5
27	418.0
28	530.0
29	624.0
30	794.5
31	1034.0
32	1458.0
33	1813.0
34	2081.0
35	2607.0
36	3280.5
37	3696.0
38	4042.0
39	5265.0
40	10828.5
41	16234.5
42	16954.0
43	15624.0
44	12956.0
45	10694.0
46	9770.0
47	9179.5
48	8077.0
49	7403.0
50	7241.0
51	6662.5
52	5473.0
53	4540.5
54	4219.0
55	3904.0
56	3302.0
57	2803.0
58	2591.0
59	2411.0
60	2291.5
61	2228.5
62	2105.0
63	2137.5
64	2228.5
65	2267.0
66	2122.0
67	1997.0
68	1949.0
69	1936.5
70	1914.0
71	1856.0
72	1826.5
73	1753.5
74	1574.5
75	1439.0
76	1404.5
77	1288.5
78	1051.5
79	896.0
80	790.5
81	588.0
82	412.5
83	334.0
84	271.5
85	178.0
86	117.0
87	87.0
88	60.0
89	27.5
90	12.0
91	2.0
92	2.0
93	1.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	172818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.75468990498675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	47.96311345087093	15.710169079609763
2	23.128290287248703	15.151199527826961
3	12.25135144684309	12.038676526750686
4	6.160124368441508	8.070918538578157
5	3.1392431897678694	5.141246860859401
6	1.6005370455428751	3.145505676492032
7	1.1253224039854433	2.5801710469974193
8	0.8320672720206338	2.1803284380099295
9	0.6041762357347278	1.7810644724507865
>10	3.1498427728509344	16.473978405027253
>50	0.022965763346641698	0.5202004420835793
>100	0.012366180263576299	0.8471339790993994
>500	0.0052997915415327	1.215151199527827
>1k	0.0035331943610217996	2.4349315464824266
>5k	0.0	0.0
>10k+	0.0017665971805108998	12.709324260204378
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	21964	12.709324260204378	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	2624	1.5183603559814371	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	1584	0.9165711905009896	No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT	888	0.5138353643717669	RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	688	0.39810667870245	No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	524	0.3032091564536102	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	335	0.19384554849610575	RNA PCR Primer, Index 24 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	224	0.12961612794963487	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	198	0.1145713988126237	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	196	0.11341411195593051	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT	194	0.11225682509923733	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.786434283465842E-4	0.0
4	0.0	0.0	0.0	0.0011572868566931684	0.0
5	0.0	0.0	0.0	0.0017359302850397528	0.0
6	0.0	0.0	0.0	0.0017359302850397528	0.0
7	0.0	0.0	0.0	0.0017359302850397528	0.0
8	0.0	0.0	0.0	0.0034718605700795055	0.0
9	0.0	0.0	0.0	0.008679651425198763	0.0
10	0.0	0.0	0.0	0.011572868566931686	0.0
11	0.0	0.0	0.0	0.014466085708664606	0.0
12	0.0	0.0	0.0	0.016780659422050943	0.0
13	0.0	0.0	0.0	0.018516589707090695	0.0
14	0.0	0.0	0.0	0.019095233135437283	0.0
15	0.0	0.0	0.0	0.02025251999213045	0.0
16	0.0	0.0	0.0	0.02025251999213045	0.0
17	0.0	0.0	0.0	0.02025251999213045	0.0
18	0.0	0.0	0.0	0.02314573713386337	0.0
19	0.0	0.0	0.0	0.027774884560636044	0.0
20	0.0	0.0	0.0	0.028932171417329212	0.0
21	0.0	0.0	0.0	0.029510814845675797	0.0
22	0.0	0.0	0.0	0.03182538855906213	0.0
23	0.0	0.0	0.0	0.03413996227244847	0.0
24	0.0	0.0	0.0	0.038190466270874565	0.0
25	0.0	0.0	0.0	0.03876910969922115	0.0
26	0.0	0.0	0.0	0.04976333483780625	0.0
27	0.0	0.0	0.0	0.09836938281891933	0.0
28	0.0	0.0	0.0	0.14871136108507216	0.0
29	0.0	0.0	0.0	0.2100475644898101	0.0
30	0.0	0.0	0.0	0.28990035760163874	0.0
31	0.0	0.0	0.0	0.3610734992882686	0.0
32	0.0	0.0	0.0	0.4461340832552165	0.0
33	0.0	0.0	0.0	0.5433461792174427	0.0
34	0.0	0.0	0.0	0.6937934705875546	0.0
35	0.0	0.0	0.0	0.9547616567718641	0.0
36	0.0	0.0	0.0	1.2869029846428035	0.0
37	0.0	0.0	0.0	1.8030529227279566	0.0
38	0.0	0.0	0.0	2.335983520235161	0.0
39	0.0	0.0	0.0	3.0043166799754655	0.0
40	0.0	0.0	0.0	3.7270423219803495	0.0
41	0.0	0.0	0.0	4.503003159393119	0.0
42	0.0	0.0	0.0	5.364603224201183	0.0
43	0.0	0.0	0.0	6.208265342730503	0.0
44	0.0	0.0	0.0	7.0704440509669135	0.0
45	0.0	0.0	0.0	7.949982062053722	0.0
46	0.0	0.0	0.0	8.861924105127938	0.0
47	0.0	0.0	0.0	9.803955606476178	0.0
48	0.0	0.0	0.0	10.659769237000775	0.0
49	0.0	0.0	0.0	11.51789744123876	0.0
50	0.0	0.0	0.0	12.267240680947587	0.0
51	0.0	0.0	0.0	13.107430938906827	0.0
52	0.0	0.0	0.0	13.917531738592045	0.0
53	0.0	0.0	0.0	14.680183777152843	0.0
54	0.0	0.0	0.0	15.474082560844357	0.0
55	0.0	0.0	0.0	16.295756229096508	0.0
56	0.0	0.0	0.0	17.041627608235252	0.0
57	0.0	0.0	0.0	17.747572590818088	0.0
58	0.0	0.0	0.0	18.503859551667073	0.0
59	0.0	0.0	0.0	19.184922866831002	0.0
60	0.0	0.0	0.0	19.86772211227997	0.0
61	0.0	0.0	0.0	20.49323565832263	0.0
62	0.0	0.0	0.0	21.14710273235427	0.0
63	0.0	0.0	0.0	21.816593178951265	0.0
64	0.0	0.0	0.0	22.4542582369892	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGATT	15	0.0022216982	70.0	22
GACGCGA	20	7.909599E-5	70.0	26
CTTTATA	100	0.0	56.000004	2
CGGAATC	20	0.0069420957	52.5	20
AGGAGCA	20	0.0069420957	52.5	60
GACGTCA	20	0.0069420957	52.5	26
TTAGAAC	20	0.0069420957	52.5	3
AATGGTC	20	0.0069420957	52.5	27
TACATTG	20	0.0069420957	52.5	5
GCGGAAT	20	0.0069420957	52.5	19
CGTCTTC	3000	0.0	52.03333	48
TATGCCG	3035	0.0	51.89456	43
CGTATGC	3050	0.0	51.86885	41
TCGTATG	3045	0.0	51.839077	40
CCGTCTT	3020	0.0	51.804634	47
ATGCCGT	3035	0.0	51.77924	44
GTATGCC	3050	0.0	51.754097	42
TCTCGTA	2995	0.0	51.652752	38
ATCTCGT	3000	0.0	51.56666	37
TCTTCTG	3035	0.0	51.5486	50
>>END_MODULE