FastQCFastQC Report
Wed 25 May 2016
SRR1779708_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779708_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76454
Sequences flagged as poor quality0
Sequence length76
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAG150.002217522169.9954235
GTTTAAG150.002217522169.9954226
TACGAAC150.002217522169.9954223
ATCATTG150.002217522169.9954259
ATGCACG207.8839985E-569.9954227
AACAGTC150.002217522169.9954270
TAGATCT2150.058.6008239
AAGTGTA308.30904E-658.32951734
GATCTCG1750.057.99621241
TGTAGCT553.45608E-1157.2689837
ATCTCGG1800.056.385242
ATCCTTT252.377666E-455.99633413
CGAAAAT252.377666E-455.99633425
TCTAGGT252.377666E-455.99633443
AGCCTAC252.377666E-455.99633435
AGATCTC2150.055.3452240
CGTGTAG700.054.99640335
AGTGTCG452.6606358E-854.44088435
GTGGTCG1550.054.19000648
TATCATT1750.053.9964758
Produced by FastQC (version 0.11.5)