FastQCFastQC Report
Wed 25 May 2016
SRR1779708_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779708_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76454
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2449732.04148899992152No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG30443.9814790593036338No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC17832.332121275538232No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT10291.3459073429774766TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC7300.9548225076516599No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC5840.7638580061213278TruSeq Adapter, Index 12 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC4130.5401941036440212RNA PCR Primer, Index 12 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2560.33484186569702046No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2390.31260627305307764TruSeq Adapter, Index 12 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1920.25113139927276534No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1710.22366390247730664No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT1570.20535223794700083RNA PCR Primer, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT1560.20404426190912184No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1260.16480498077275224No Hit
TCTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT950.12425772359850368No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT910.11902581944698773No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAAG150.00221694770.069
ACAAACC150.00221694770.070
CCGTGAA150.00221694770.014
AAAACGG150.00221694770.068
AATATCC150.00221694770.070
AACATCA150.00221694770.070
TGACATC150.00221694770.033
GACATCT150.00221694770.034
AAAGAGA150.00221694770.070
TCTTTAT850.061.764711
TCTTAAT252.3768976E-455.9999961
AAACATC252.3768976E-455.99999669
TTTACAC452.6591806E-854.4444432
CTTTACA452.6591806E-854.4444431
AGACTGC200.00692736352.50000425
TATGCTG200.00692736352.50000443
TTGACAT200.00692736352.50000432
CTTGACA200.00692736352.50000431
CTTTATA1000.052.4999962
GCTTGAA37450.051.77570356
Produced by FastQC (version 0.11.5)