FastQCFastQC Report
Wed 25 May 2016
SRR1779707_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779707_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112855
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGA900.054.43720621
CTGTGTA452.6771886E-854.43720634
AGATATC200.006939530452.49302340
AAATACC200.006939530452.49302346
GCGGTAT200.006939530452.49302354
ACTCTCG200.006939530452.49302341
ATTCGTG200.006939530452.49302332
TCGGTGG1400.052.49302345
TACACGC200.006939530452.49302341
AGTGTTG200.006939530452.49302335
CGAAGTG407.430626E-752.49302332
TCTCGGT1100.050.9023343
CTCGGTG1400.049.99335544
CGGTGGT1200.049.57674446
CCGTATC850.049.40519755
AGATCTC1150.048.68918240
CGTGTAG950.047.8883735
TAGATCT1700.047.3466539
GCGTGTA305.8606075E-446.66046534
CGATCCA305.8606075E-446.66046525
Produced by FastQC (version 0.11.5)