##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779707_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	112855
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.9797350582606	32.0	21.0	34.0	12.0	34.0
2	26.18615923087147	31.0	21.0	34.0	12.0	34.0
3	25.47183554118116	31.0	21.0	34.0	11.0	34.0
4	26.829303088033317	32.0	21.0	34.0	12.0	34.0
5	26.692481502813344	32.0	21.0	34.0	11.0	34.0
6	28.426582783217402	34.0	21.0	38.0	11.0	38.0
7	29.44923131451863	34.0	22.0	38.0	12.0	38.0
8	28.586371893137212	34.0	21.0	38.0	11.0	38.0
9	30.14502680430641	36.0	25.0	38.0	12.0	38.0
10-11	29.59281378760356	36.0	22.0	38.0	11.0	38.0
12-13	29.19311505914669	35.0	22.0	38.0	11.0	38.0
14-15	28.718395285986446	34.0	21.0	38.0	11.0	38.0
16-17	28.554020645961632	33.5	22.0	37.5	11.0	38.0
18-19	29.09171946302778	34.0	21.5	38.0	11.0	38.0
20-21	28.09264986044039	32.5	21.5	37.5	11.0	38.0
22-23	28.13282973727349	33.0	16.5	37.5	11.0	38.0
24-25	28.838939346949626	34.0	21.5	37.5	11.0	38.0
26-27	27.676390058039075	32.5	16.0	37.5	11.0	38.0
28-29	29.220322537769704	35.0	21.0	38.0	11.0	38.0
30-31	29.659509990696023	36.0	22.0	38.0	11.0	38.0
32-33	29.78618138319082	36.0	22.0	38.0	11.0	38.0
34-35	29.826742279916708	36.0	22.0	38.0	11.0	38.0
36-37	28.34564706924815	32.5	21.5	38.0	11.0	38.0
38-39	27.272340614062294	31.0	21.0	37.0	11.0	38.0
40-41	27.692326436577908	32.5	21.0	37.0	11.0	38.0
42-43	28.502410172345044	33.5	21.5	37.5	11.0	38.0
44-45	29.885109210934385	36.0	23.0	38.0	11.0	38.0
46-47	28.812112888219396	34.5	22.0	38.0	11.0	38.0
48-49	28.18001417748438	32.5	16.0	37.5	11.0	38.0
50-51	26.594280271144388	29.0	11.0	37.0	11.0	38.0
52-53	28.929387266846838	35.0	21.0	37.5	11.0	38.0
54-55	28.279451508572947	33.0	21.0	38.0	11.0	38.0
56-57	28.65391431482876	35.0	22.0	38.0	11.0	38.0
58-59	29.438943777413495	35.5	21.5	38.0	11.0	38.0
60-61	29.937747552168712	37.0	23.0	38.0	11.0	38.0
62-63	29.879907846351514	36.5	22.5	38.0	11.0	38.0
64-65	29.62499224668823	36.5	22.0	38.0	11.0	38.0
66-67	28.134641796996146	34.0	21.0	38.0	11.0	38.0
68-69	28.078109078020468	34.0	21.0	38.0	11.0	38.0
70-71	27.746931903770324	32.5	20.5	37.5	11.0	38.0
72-73	26.78716051570599	29.5	15.0	37.0	11.0	38.0
74-75	28.12867839262771	33.5	20.5	37.5	11.0	38.0
76	28.32130610074875	34.0	21.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	1.0
5	0.0
6	0.0
7	3.0
8	0.0
9	0.0
10	1.0
11	15.0
12	78.0
13	411.0
14	1086.0
15	1853.0
16	3003.0
17	4681.0
18	6155.0
19	6330.0
20	5630.0
21	4190.0
22	3098.0
23	2494.0
24	2083.0
25	1951.0
26	2027.0
27	2158.0
28	2395.0
29	2813.0
30	3355.0
31	4112.0
32	5018.0
33	6782.0
34	9077.0
35	11674.0
36	13235.0
37	7143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.59443533737982	17.342607771033627	12.625935935492446	36.4370209560941
2	17.721855478268576	16.149926897346152	42.69017766160117	23.438039962784103
3	17.938380016481617	15.205621472180624	40.258566453705264	26.597432057632496
4	14.00735457002348	20.321651676930575	30.096141065969604	35.57485268707634
5	13.929378405919099	25.727703690576405	41.458508705861504	18.88440919764299
6	25.288201674715342	32.411501484205395	17.029817021842184	25.270479819237075
7	25.14376855256745	22.169155110540075	20.23215630676532	32.454920030127155
8	20.93967106195945	31.86232808733872	19.001665957748475	28.196334892953352
9	20.7580107755565	14.095952077130299	19.755777683255353	45.39025946405785
10-11	23.371260456543315	21.707518077413866	28.09442790301999	26.82679356302283
12-13	22.009198376548568	16.79501267213725	31.21422115094908	29.9815678003651
14-15	24.309260079751883	21.156845369960124	23.08019494904741	31.453699601240587
16-17	27.07244989742431	19.072432173762778	24.3687053308343	29.486412597978617
18-19	21.565482467411627	18.738646131487766	28.766382801492284	30.92948859960832
20-21	26.848061108748052	18.781901318587835	25.08329788742379	29.28673968524032
22-23	28.019850237050818	17.803624440604366	24.742345695422927	29.434179626921885
24-25	22.107221976074435	23.031900753212227	21.879042977403635	32.9818342933097
26-27	25.641218769799252	22.235415568246765	24.57787446334342	27.545491198610563
28-29	23.49201144892733	22.446366383397283	24.570886760183964	29.490735407491425
30-31	22.922832887297734	21.999007496942735	25.45150027471067	29.62665934104886
32-33	23.5729635127052	21.341190544761737	25.541106360353577	29.544739582179492
34-35	23.253062761691666	21.59950375506768	25.457808103856976	29.68962537938368
36-37	26.27728817464432	17.997899801943348	30.222470346887143	25.50234167652519
38-39	25.90290178076911	16.489505469916566	34.45715121250925	23.150441536805072
40-41	24.328731191180903	22.266185775304397	24.7412402743562	28.6638427591585
42-43	19.501021262643942	22.986163109600753	27.400853341367558	30.111962286387744
44-45	19.643498026079676	19.374105532736948	30.490976680712294	30.491419760471082
46-47	25.234386076846732	18.479724939741953	28.30488799092585	27.981000992485466
48-49	24.561799943286545	19.29276548986247	30.046168297178504	26.099266269672476
50-51	30.59735219055721	17.48192258613356	28.347866156245573	23.57285906706366
52-53	18.828205309985467	21.519974478040478	29.47077381163376	30.181046400340293
54-55	23.76800035446852	19.813460942000088	27.132349683193763	29.286189020337634
56-57	24.05171535288462	20.300225523600226	26.267517955488994	29.38054116802616
58-59	19.63481042664528	22.202480382469837	28.286019380480393	29.87668981040449
60-61	19.395016216791024	20.505379012104992	28.028906651542812	32.07069811956117
62-63	19.275469557935406	22.267613684196537	27.619621517916087	30.837295239951967
64-65	19.83641712746349	23.370374308804763	26.484297462072874	30.308911101658868
66-67	18.244455767995216	27.870261647872034	21.257504929437047	32.627777654695706
68-69	18.723491009657362	27.545860276291823	21.69515713709552	32.035491576955295
70-71	18.757947813603558	28.261206694108314	21.14351037480449	31.83733511748364
72-73	18.06005396615817	29.519665746554008	20.543915071977032	31.87636521531079
74-75	20.1399227279172	25.661952360697576	24.338047639302424	29.8600772720828
76	20.26673756037042	24.759626035712703	25.11232221188356	29.861314192033323
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	1.0
6	1.0
7	1.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.0
14	1.0
15	3.0
16	3.5
17	3.0
18	6.5
19	10.0
20	21.0
21	32.0
22	35.5
23	61.0
24	107.5
25	132.0
26	172.0
27	265.5
28	376.0
29	433.0
30	532.0
31	760.0
32	1048.5
33	1208.0
34	1340.0
35	1720.5
36	2204.0
37	2439.0
38	2740.5
39	3347.5
40	3951.0
41	4564.5
42	4880.0
43	4948.5
44	5241.5
45	5519.5
46	5573.0
47	5636.0
48	5575.5
49	5227.5
50	5003.0
51	4866.5
52	4498.5
53	4089.5
54	3912.0
55	3784.0
56	3529.0
57	3242.5
58	3083.0
59	3053.5
60	2939.5
61	2850.0
62	2845.0
63	2743.5
64	2586.5
65	2415.5
66	2256.5
67	2213.0
68	2139.0
69	2008.0
70	1878.0
71	1805.0
72	1692.0
73	1532.0
74	1346.5
75	1208.0
76	1046.0
77	865.5
78	710.0
79	573.0
80	484.0
81	333.5
82	226.5
83	181.0
84	140.0
85	74.5
86	42.0
87	34.0
88	26.0
89	16.5
90	9.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0017721855478268576
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0062026494173940015
9	0.0062026494173940015
10-11	0.0062026494173940015
12-13	0.007974834965220858
14-15	0.004430463869567144
16-17	0.010190066900004431
18-19	0.00708874219130743
20-21	0.0062026494173940015
22-23	0.008860927739134288
24-25	0.004430463869567144
26-27	0.0031013247086970007
28-29	0.005316556643480573
30-31	0.007974834965220858
32-33	0.006645695804350716
34-35	0.006645695804350716
36-37	0.007531788578264144
38-39	0.009303974126091002
40-41	0.007974834965220858
42-43	0.003987417482610429
44-45	0.007531788578264144
46-47	0.0062026494173940015
48-49	0.0062026494173940015
50-51	0.0062026494173940015
52-53	0.009747020513047717
54-55	0.008860927739134288
56-57	0.005759603030437287
58-59	0.008417881352177574
60-61	0.007974834965220858
62-63	0.008417881352177574
64-65	0.0062026494173940015
66-67	0.01107615967391786
68-69	0.03500066456958044
70-71	0.008417881352177574
72-73	0.005759603030437287
74-75	0.0062026494173940015
76	0.008860927739134288
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	112855.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.58334362436415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.32563357244038	94.00035110785961
2	2.0941707358644575	4.045240235801688
3	0.40921791320513184	1.185709029850094
4	0.10098930085273472	0.39015537386575877
5	0.04299300773746589	0.20762042198763073
6	0.018996814783688712	0.11008655340128447
7	0.003999327641069977	0.027038790507671547
8	0.0019996637412118775	0.01545073682005187
9	9.998318670853333E-4	0.00869103943067631
>10	9.99831866772851E-4	0.009656710475511174
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.860927739134288E-4	0.0
2	0.0	0.0	0.0	0.0026582783217402864	0.0
3	0.0	0.0	0.0	0.0026582783217402864	0.0
4	0.0	0.0	0.0	0.005316556643480573	0.0
5	0.0	0.0	0.0	0.009747020513047716	0.0
6	0.0	0.0	0.0	0.010633113286961145	0.0
7	0.0	0.0	0.0	0.010633113286961145	0.0
8	0.0	0.0	0.0	0.010633113286961145	0.0
9	0.0	0.0	0.0	0.012405298834788003	0.0
10	0.0	0.0	0.0	0.012405298834788003	0.0
11	0.0	0.0	0.0	0.012405298834788003	0.0
12	0.0	0.0	0.0	0.013291391608701431	0.0
13	0.0	0.0	0.0	0.013291391608701431	0.0
14	0.0	0.0	0.0	0.013291391608701431	0.0
15	0.0	0.0	0.0	0.013291391608701431	0.0
16	0.0	0.0	0.0	0.013291391608701431	0.0
17	0.0	0.0	0.0	0.013291391608701431	0.0
18	0.0	0.0	0.0	0.016835762704355144	0.0
19	0.0	0.0	0.0	0.02126622657392229	0.0
20	0.0	0.0	0.0	0.025696690443489434	0.0
21	0.0	0.0	0.0	0.026582783217402862	0.0
22	0.0	0.0	0.0	0.04430463869567144	0.0
23	0.0	0.0	0.0	0.04607682424349829	0.0
24	0.0	0.0	0.0	0.05493775198263258	0.0
25	0.0	0.0	0.0	0.05759603030437287	0.0
26	0.0	0.0	0.0	0.06822914359133402	0.0
27	0.0	0.0	0.0	0.10455894732178458	0.0
28	0.0	0.0	0.0	0.15063577156528288	0.0
29	0.0	0.0	0.0	0.1851933897479066	0.0
30	0.0	0.0	0.0	0.24278942005227946	0.0
31	0.0	0.0	0.0	0.2773470382349032	0.0
32	0.0	0.0	0.0	0.3172212130610075	0.0
33	0.0	0.0	0.0	0.3987417482610429	0.0
34	0.0	0.0	0.0	0.48203446900890523	0.0
35	0.0	0.0	0.0	0.6822914359133401	0.0
36	0.0	0.0	0.0	0.8320411147047095	0.0
37	0.0	0.0	0.0	1.2352133268353196	0.0
38	0.0	0.0	0.0	1.5781312303398165	0.0
39	0.0	0.0	0.0	2.0247219883921845	0.0
40	0.0	0.0	0.0	2.497009436888042	0.0
41	0.0	0.0	0.0	2.9028399273403926	0.0
42	0.0	0.0	0.0	3.304239953923176	0.0
43	0.0	0.0	0.0	3.715387001019007	0.0
44	0.0	0.0	0.0	4.266536706393159	0.0
45	0.0	0.0	0.0	4.627176465375925	0.0
46	0.0	0.0	0.0	5.003765894289132	0.0
47	0.0	0.0	0.0	5.4840281777502105	0.0
48	0.0	0.0	0.0	5.830490452350361	0.0
49	0.0	0.0	0.0	6.1140401400026585	0.0
50	0.0	0.0	0.0	6.480882548402818	0.0
51	0.0	0.0	0.0	6.941650790837801	0.0
52	0.0	0.0	0.0	7.296973993177086	0.0
53	0.0	0.0	0.0	7.740906472907714	0.0
54	0.0	0.0	0.0	8.185725045412255	0.0
55	0.0	0.0	0.0	8.497629701829782	0.0
56	0.0	0.0	0.0	8.864472110229942	0.0
57	0.0	0.0	0.0	9.135616499047451	0.0
58	0.0	0.0	0.0	9.38106419742147	0.0
59	0.0	0.0	0.0	9.677905276682468	0.0
60	0.0	0.0	0.0	9.872845686943423	0.0
61	0.0	0.0	0.0	10.053608612821762	0.0
62	0.0	0.0	0.0	10.192725178326171	0.0
63	0.0	0.0	0.0	10.400956980195826	0.0
64	0.0	0.0	0.0	10.611847060387223	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTACGA	90	0.0	54.437206	21
CTGTGTA	45	2.6771886E-8	54.437206	34
AGATATC	20	0.0069395304	52.493023	40
AAATACC	20	0.0069395304	52.493023	46
GCGGTAT	20	0.0069395304	52.493023	54
ACTCTCG	20	0.0069395304	52.493023	41
ATTCGTG	20	0.0069395304	52.493023	32
TCGGTGG	140	0.0	52.493023	45
TACACGC	20	0.0069395304	52.493023	41
AGTGTTG	20	0.0069395304	52.493023	35
CGAAGTG	40	7.430626E-7	52.493023	32
TCTCGGT	110	0.0	50.90233	43
CTCGGTG	140	0.0	49.993355	44
CGGTGGT	120	0.0	49.576744	46
CCGTATC	85	0.0	49.405197	55
AGATCTC	115	0.0	48.689182	40
CGTGTAG	95	0.0	47.88837	35
TAGATCT	170	0.0	47.34665	39
GCGTGTA	30	5.8606075E-4	46.660465	34
CGATCCA	30	5.8606075E-4	46.660465	25
>>END_MODULE