FastQCFastQC Report
Wed 25 May 2016
SRR1779707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779707_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112855
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT2617223.190820078862256TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG31822.81954720659253TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC20461.8129458154268752RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT10460.9268530415134464TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC8060.7141907757742235RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC6590.5839351380089495TruSeq Adapter, Index 5 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC3770.33405697576536264RNA PCR Primer, Index 5 (95% over 24bp)
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG3520.31190465641752696TruSeq Adapter, Index 5 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC2490.22063710070444376TruSeq Adapter, Index 5 (95% over 24bp)
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC2440.21620663683487662No Hit
TATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCT1940.17190199813920518RNA PCR Primer, Index 5 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT1880.1665854414957246No Hit
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1810.16038279207833062TruSeq Adapter, Index 5 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT1440.12759735944353373No Hit
CCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1240.10987550396526517No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAA150.002219694470.000011
AGTAGCC150.002219694470.0000125
AGATCTC200.006935884252.535
CTCAAAT200.006935884252.551
AAATACC200.006935884252.570
GTGGGTA200.006935884252.534
CATAAGT200.006935884252.53
AACTCTT200.006935884252.570
AACTCTG200.006935884252.57
CAGTAGC200.006935884252.524
TCTCGTA37600.049.70744738
CCGTCTT38350.049.4654547
ATCTCGT37800.049.44444337
ATGCCGT38450.049.42782644
CTCGTAT38100.049.4225739
TATGCCG38450.049.336843
GATCTCG38000.049.27631836
TTGAAAA38300.049.25587558
CGTATGC38450.049.2457741
TGCTTGA38600.049.23575255
Produced by FastQC (version 0.11.5)