##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779707_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	112855
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.63486775065349	34.0	34.0	34.0	34.0	34.0
2	33.64834522174472	34.0	34.0	34.0	34.0	34.0
3	33.66850383235125	34.0	34.0	34.0	34.0	34.0
4	33.70159939745692	34.0	34.0	34.0	34.0	34.0
5	33.70890080191396	34.0	34.0	34.0	34.0	34.0
6	37.3627309379292	38.0	38.0	38.0	37.0	38.0
7	37.33906339993797	38.0	38.0	38.0	37.0	38.0
8	37.22934739267201	38.0	38.0	38.0	36.0	38.0
9	37.25889858668202	38.0	38.0	38.0	36.0	38.0
10-11	37.16176509680564	38.0	38.0	38.0	36.0	38.0
12-13	37.140919764299326	38.0	38.0	38.0	36.0	38.0
14-15	37.15976695760046	38.0	38.0	38.0	36.0	38.0
16-17	37.07069248150282	38.0	38.0	38.0	35.5	38.0
18-19	37.03870896282841	38.0	38.0	38.0	35.0	38.0
20-21	37.10201586106065	38.0	38.0	38.0	36.0	38.0
22-23	37.06818926941651	38.0	38.0	38.0	36.0	38.0
24-25	36.96513224934651	38.0	38.0	38.0	35.0	38.0
26-27	36.84440653936467	38.0	38.0	38.0	34.5	38.0
28-29	36.846271764653764	38.0	37.5	38.0	34.5	38.0
30-31	36.56093660006203	38.0	37.0	38.0	34.0	38.0
32-33	36.42848345221745	38.0	37.0	38.0	34.0	38.0
34-35	36.33604182357893	38.0	37.0	38.0	34.0	38.0
36-37	36.587989012449604	38.0	37.0	38.0	34.0	38.0
38-39	35.84810154623189	38.0	37.0	38.0	32.0	38.0
40-41	36.50847547738248	38.0	37.0	38.0	34.0	38.0
42-43	36.85632891763768	38.0	37.0	38.0	35.0	38.0
44-45	36.82681759780249	38.0	37.0	38.0	34.5	38.0
46-47	36.85324974524833	38.0	37.0	38.0	35.0	38.0
48-49	36.82442514731292	38.0	37.0	38.0	34.5	38.0
50-51	36.70437286783926	38.0	37.0	38.0	34.0	38.0
52-53	36.691816933232914	38.0	37.0	38.0	34.0	38.0
54-55	36.67389127641664	38.0	37.0	38.0	34.0	38.0
56-57	36.68106862788534	38.0	37.0	38.0	34.0	38.0
58-59	36.556018785166806	38.0	37.0	38.0	34.0	38.0
60-61	36.59910061583447	38.0	37.0	38.0	34.0	38.0
62-63	36.66102963980329	38.0	37.0	38.0	34.0	38.0
64-65	36.70857737805148	38.0	37.0	38.0	34.0	38.0
66-67	36.5992423906783	38.0	37.0	38.0	34.0	38.0
68-69	36.300305702007	38.0	37.0	38.0	34.0	38.0
70-71	34.20486464932878	38.0	34.0	38.0	21.0	38.0
72-73	30.693722032696822	38.0	23.0	38.0	11.0	38.0
74-75	30.209937530459438	38.0	23.0	38.0	11.0	38.0
76	29.909769172832394	37.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	7.0
22	10.0
23	17.0
24	48.0
25	89.0
26	169.0
27	317.0
28	549.0
29	897.0
30	1314.0
31	2100.0
32	3217.0
33	5186.0
34	8943.0
35	19172.0
36	21694.0
37	49120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.883080058482125	12.856320056709938	11.591865668335474	46.668734216472465
2	15.206238093128349	12.793407469762084	49.42536883611714	22.574985600992424
3	14.946612910371716	14.501794337867175	47.75508395729033	22.796508794470782
4	11.759337202605112	35.306366576580565	28.048380665455674	24.885915555358647
5	12.35301936112711	26.73430508174206	47.89242833724691	13.02024721988392
6	23.38310221080147	48.37977936289929	14.080014177484381	14.15710424881485
7	21.313189490939703	22.69017766160117	15.798148066102522	40.198484781356605
8	18.604403881086352	48.60661911302113	12.82619290239688	19.962784103495636
9	17.354126977094502	14.60103672854548	12.780116078153384	55.26472021620664
10-11	20.017721855478268	30.35753843427407	28.40370386779496	21.221035842452704
12-13	19.593726463160692	14.144255903593105	31.091666297461344	35.17035133578486
14-15	18.869345620486467	14.01311417305392	16.872978600859508	50.244561605600104
16-17	33.36981081919277	28.2149661069514	14.063621461166983	24.351601612688846
18-19	34.17128173319747	18.399716450312347	14.526161889149794	32.902839927340395
20-21	19.980949005360863	15.699791768198128	14.310841345088832	50.00841788135217
22-23	19.5844224890346	29.705374152673787	14.211598954410526	36.49860440388109
24-25	33.675069779805945	29.94196092330867	13.99406317841478	22.388906118470604
26-27	33.831908200788625	31.817376279296443	14.718444021089008	19.632271498825926
28-29	19.511762881573702	32.23516902219662	14.961676487528244	33.29139160870143
30-31	18.59731513889504	32.23694120774445	14.783128793584687	34.38261485977582
32-33	18.91719463027779	45.84776926144167	15.254973195693589	19.98006291258695
34-35	33.31354392804926	20.24101723450445	28.535288644721103	17.910150192725176
36-37	33.97988569403216	27.629701829781578	19.482964866421515	18.907447609764745
38-39	19.48385095919543	17.992999867086084	30.4718444021089	32.05130477160959
40-41	18.241105843781842	14.414071153249743	32.632581631296794	34.71224137167162
42-43	31.917061716361705	14.390146648354083	31.6561073944442	22.036684240840014
44-45	18.61060653050374	28.79845819857339	33.250188294714455	19.340746976208408
46-47	31.27464445527447	16.037393115059146	20.843560320765583	31.844402108900805
48-49	30.551149705374154	14.596606264675913	18.02489920694697	36.82734482300297
50-51	19.004031722121304	14.998449337645651	32.403083602853215	33.59443533737982
52-53	15.786628860041645	14.827876478667317	46.724115014842056	22.661379646448985
54-55	15.693146072393779	14.674582428780294	35.64795534092419	33.98431615790173
56-57	29.88303575384343	14.797749324354259	21.42793850516149	33.89127641664082
58-59	17.70501971556422	16.06131761995481	43.584688316866774	22.648974347614196
60-61	30.2077887554827	29.6437907048868	20.575074210269815	19.57334632936068
62-63	16.52075672322892	47.915466749368655	15.557573877985027	20.006202649417393
64-65	15.21687120641531	49.64511984404767	15.099020867484825	20.038988082052192
66-67	15.61339772274157	50.13601524079571	14.500022152319348	19.75056488414337
68-69	15.475610296398031	50.29595498648709	14.308183066767091	19.920251650347794
70-71	15.687386469363343	47.93628992955563	14.991803641841301	21.384519959239732
72-73	16.17695272695051	40.556909308404585	17.42324221345975	25.842895751185146
74-75	17.01298125913783	37.536662088520664	19.00491781489522	26.44543883744628
76	17.102476629303087	38.07097603119047	18.86314297106907	25.963404368437377
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	1.0
15	1.0
16	2.5
17	3.0
18	6.0
19	10.5
20	14.0
21	16.0
22	28.5
23	54.0
24	92.0
25	117.0
26	141.0
27	190.0
28	256.0
29	297.0
30	369.0
31	483.0
32	668.5
33	812.0
34	904.5
35	1114.0
36	1365.5
37	1500.0
38	1886.0
39	7728.5
40	13809.5
41	12825.5
42	11217.0
43	9379.0
44	6296.5
45	4470.5
46	3889.0
47	3631.0
48	3106.0
49	2836.5
50	2834.0
51	2656.0
52	2231.0
53	1934.0
54	1884.0
55	1889.5
56	1835.0
57	1782.0
58	1789.0
59	1827.0
60	1838.5
61	1821.0
62	1830.0
63	1824.0
64	1874.0
65	1958.5
66	1905.5
67	1824.0
68	1847.0
69	1899.5
70	1823.0
71	1717.0
72	1655.0
73	1577.5
74	1436.0
75	1310.0
76	1306.5
77	1142.0
78	885.0
79	789.0
80	742.5
81	580.0
82	376.0
83	288.0
84	235.0
85	134.0
86	73.5
87	61.0
88	47.5
89	25.5
90	10.5
91	5.5
92	7.0
93	4.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	112855.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.36857028930929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.79802830428519	15.620929511319837
2	23.971074263438886	15.038766558858713
3	12.434676986525805	11.701741172300741
4	6.008304850145476	7.538877320455452
5	3.0931329623456962	4.851357937176022
6	1.5875257761080195	2.9879048336360814
7	0.8756814779243524	1.9228213193921404
8	0.5762549080534448	1.4461034070267158
9	0.32484958052032425	0.9171060210003988
>10	1.2570266376656025	5.460989765628462
>50	0.031072568571509283	0.6459616321828896
>100	0.025423010649416683	1.8191484648442693
>500	0.005649557922092596	1.298125913783173
>1k	0.008474336883138894	5.5593460635328515
>5k	0.0	0.0
>10k+	0.002824778961046298	23.190820078862256
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	26172	23.190820078862256	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	3182	2.81954720659253	TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	2046	1.8129458154268752	RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT	1046	0.9268530415134464	TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	806	0.7141907757742235	RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	659	0.5839351380089495	TruSeq Adapter, Index 5 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	377	0.33405697576536264	RNA PCR Primer, Index 5 (95% over 24bp)
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	352	0.31190465641752696	TruSeq Adapter, Index 5 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	249	0.22063710070444376	TruSeq Adapter, Index 5 (95% over 24bp)
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	244	0.21620663683487662	No Hit
TATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCT	194	0.17190199813920518	RNA PCR Primer, Index 5 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT	188	0.1665854414957246	No Hit
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	181	0.16038279207833062	TruSeq Adapter, Index 5 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT	144	0.12759735944353373	No Hit
CCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	124	0.10987550396526517	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004430463869567144	0.0
2	0.0	0.0	0.0	0.0062026494173940015	0.0
3	0.0	0.0	0.0	0.0062026494173940015	0.0
4	0.0	0.0	0.0	0.008860927739134288	0.0
5	0.0	0.0	0.0	0.012405298834788003	0.0
6	0.0	0.0	0.0	0.01417748438261486	0.0
7	0.0	0.0	0.0	0.01417748438261486	0.0
8	0.0	0.0	0.0	0.01417748438261486	0.0
9	0.0	0.0	0.0	0.015949669930441716	0.0
10	0.0	0.0	0.0	0.015949669930441716	0.0
11	0.0	0.0	0.0	0.015949669930441716	0.0
12	0.0	0.0	0.0	0.016835762704355144	0.0
13	0.0	0.0	0.0	0.016835762704355144	0.0
14	0.0	0.0	0.0	0.016835762704355144	0.0
15	0.0	0.0	0.0	0.017721855478268576	0.0
16	0.0	0.0	0.0	0.017721855478268576	0.0
17	0.0	0.0	0.0	0.017721855478268576	0.0
18	0.0	0.0	0.0	0.02215231934783572	0.0
19	0.0	0.0	0.0	0.026582783217402862	0.0
20	0.0	0.0	0.0	0.03544371095653715	0.0
21	0.0	0.0	0.0	0.03810198927827744	0.0
22	0.0	0.0	0.0	0.04164636037393115	0.0
23	0.0	0.0	0.0	0.04519073146958487	0.0
24	0.0	0.0	0.0	0.05139338088697887	0.0
25	0.0	0.0	0.0	0.05493775198263258	0.0
26	0.0	0.0	0.0	0.06822914359133402	0.0
27	0.0	0.0	0.0	0.10987550396526516	0.0
28	0.0	0.0	0.0	0.1692437198174649	0.0
29	0.0	0.0	0.0	0.2374728634087989	0.0
30	0.0	0.0	0.0	0.3145629347392672	0.0
31	0.0	0.0	0.0	0.41734969651322495	0.0
32	0.0	0.0	0.0	0.4873510256523858	0.0
33	0.0	0.0	0.0	0.6379867972176687	0.0
34	0.0	0.0	0.0	0.8603960834699393	0.0
35	0.0	0.0	0.0	1.1598954410526783	0.0
36	0.0	0.0	0.0	1.5719285809224226	0.0
37	0.0	0.0	0.0	2.2302955119401	0.0
38	0.0	0.0	0.0	2.8062558149838286	0.0
39	0.0	0.0	0.0	3.4796863231580346	0.0
40	0.0	0.0	0.0	4.18147180009747	0.0
41	0.0	0.0	0.0	4.7273049488281425	0.0
42	0.0	0.0	0.0	5.307695715741438	0.0
43	0.0	0.0	0.0	5.880997740463426	0.0
44	0.0	0.0	0.0	6.6075938150724385	0.0
45	0.0	0.0	0.0	7.264188560542289	0.0
46	0.0	0.0	0.0	7.903061450533871	0.0
47	0.0	0.0	0.0	8.598644278055913	0.0
48	0.0	0.0	0.0	9.251694652430109	0.0
49	0.0	0.0	0.0	9.81524965663905	0.0
50	0.0	0.0	0.0	10.290195383456648	0.0
51	0.0	0.0	0.0	10.824509326126446	0.0
52	0.0	0.0	0.0	11.291480217978823	0.0
53	0.0	0.0	0.0	11.772628594213813	0.0
54	0.0	0.0	0.0	12.259093527092286	0.0
55	0.0	0.0	0.0	12.75884985157946	0.0
56	0.0	0.0	0.0	13.295822072570997	0.0
57	0.0	0.0	0.0	13.757476407779894	0.0
58	0.0	0.0	0.0	14.190775774223562	0.0
59	0.0	0.0	0.0	14.616100305702007	0.0
60	0.0	0.0	0.0	15.031677816667406	0.0
61	0.0	0.0	0.0	15.449913605954544	0.0
62	0.0	0.0	0.0	15.889415621815605	0.0
63	0.0	0.0	0.0	16.35018386425059	0.0
64	0.0	0.0	0.0	16.803863364494262	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAA	15	0.0022196944	70.00001	1
AGTAGCC	15	0.0022196944	70.00001	25
AGATCTC	20	0.0069358842	52.5	35
CTCAAAT	20	0.0069358842	52.5	51
AAATACC	20	0.0069358842	52.5	70
GTGGGTA	20	0.0069358842	52.5	34
CATAAGT	20	0.0069358842	52.5	3
AACTCTT	20	0.0069358842	52.5	70
AACTCTG	20	0.0069358842	52.5	7
CAGTAGC	20	0.0069358842	52.5	24
TCTCGTA	3760	0.0	49.707447	38
CCGTCTT	3835	0.0	49.46545	47
ATCTCGT	3780	0.0	49.444443	37
ATGCCGT	3845	0.0	49.427826	44
CTCGTAT	3810	0.0	49.42257	39
TATGCCG	3845	0.0	49.3368	43
GATCTCG	3800	0.0	49.276318	36
TTGAAAA	3830	0.0	49.255875	58
CGTATGC	3845	0.0	49.24577	41
TGCTTGA	3860	0.0	49.235752	55
>>END_MODULE