##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779706_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5910 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.25736040609137 34.0 32.0 34.0 21.0 34.0 2 28.760913705583757 34.0 23.0 34.0 12.0 34.0 3 27.905583756345177 34.0 23.0 34.0 11.0 34.0 4 30.383925549915396 34.0 32.0 34.0 21.0 34.0 5 30.106429780033842 34.0 31.0 34.0 11.0 34.0 6 32.40372250423012 37.0 31.0 38.0 12.0 38.0 7 33.45482233502538 37.0 34.0 38.0 21.0 38.0 8 32.793231810490695 37.0 34.0 38.0 12.0 38.0 9 33.8927241962775 38.0 34.0 38.0 21.0 38.0 10-11 33.77301184433164 38.0 34.0 38.0 21.0 38.0 12-13 32.69957698815567 37.5 31.5 38.0 16.0 38.0 14-15 32.8321489001692 37.0 32.5 38.0 16.0 38.0 16-17 32.39729272419628 37.5 30.0 38.0 16.0 38.0 18-19 33.06429780033841 37.0 33.0 38.0 16.0 38.0 20-21 32.10837563451777 37.0 30.0 38.0 16.0 38.0 22-23 32.00059221658206 37.0 29.0 38.0 16.0 38.0 24-25 33.293401015228426 37.5 33.0 38.0 21.0 38.0 26-27 31.730372250423013 37.0 28.5 38.0 11.0 38.0 28-29 33.96116751269035 38.0 34.5 38.0 21.0 38.0 30-31 34.36311336717428 38.0 36.0 38.0 22.0 38.0 32-33 34.43248730964467 38.0 36.0 38.0 22.5 38.0 34-35 34.49813874788494 38.0 36.5 38.0 22.0 38.0 36-37 32.42690355329949 37.0 30.0 38.0 16.0 38.0 38-39 30.650338409475467 36.5 26.0 38.0 11.0 38.0 40-41 31.968274111675125 36.5 29.5 38.0 15.0 38.0 42-43 32.951099830795265 37.5 32.0 38.0 16.0 38.0 44-45 34.348307952622676 38.0 36.0 38.0 22.0 38.0 46-47 32.94796954314721 37.5 32.0 38.0 17.0 38.0 48-49 32.448815566835876 37.0 30.5 38.0 16.0 38.0 50-51 30.385532994923857 36.0 24.5 38.0 11.0 38.0 52-53 33.65710659898477 37.5 34.5 38.0 21.5 38.0 54-55 32.83130287648054 37.5 30.5 38.0 16.0 38.0 56-57 33.17571912013536 37.5 33.5 38.0 16.0 38.0 58-59 34.249323181049064 38.0 35.5 38.0 22.0 38.0 60-61 34.48874788494078 38.0 37.0 38.0 22.5 38.0 62-63 34.40846023688663 38.0 36.5 38.0 22.5 38.0 64-65 34.14729272419628 38.0 35.5 38.0 22.0 38.0 66-67 32.21116751269035 37.5 29.5 38.0 11.0 38.0 68-69 32.19653130287648 37.0 29.5 38.0 11.0 38.0 70-71 31.706091370558376 37.0 28.5 38.0 11.0 38.0 72-73 30.71497461928934 36.5 25.5 38.0 11.0 38.0 74-75 32.87648054145516 37.0 32.0 38.0 16.0 38.0 76 33.28426395939086 37.0 34.0 38.0 21.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 3.0 13 10.0 14 36.0 15 43.0 16 71.0 17 66.0 18 96.0 19 114.0 20 90.0 21 79.0 22 70.0 23 78.0 24 49.0 25 68.0 26 60.0 27 71.0 28 70.0 29 113.0 30 113.0 31 185.0 32 275.0 33 412.0 34 637.0 35 1051.0 36 1325.0 37 724.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.82402707275804 20.93062605752961 13.282571912013536 31.962774957698812 2 20.65989847715736 17.732656514382402 38.595600676818954 23.011844331641285 3 19.695431472081218 19.407783417935704 34.991539763113366 25.90524534686971 4 12.910321489001692 19.153976311336717 33.434856175972925 34.500846023688666 5 13.637901861252116 30.896785109983078 38.52791878172589 16.937394247038917 6 25.76988155668359 35.5668358714044 16.920473773265652 21.74280879864636 7 25.60067681895093 26.76818950930626 21.962774957698816 25.668358714043993 8 22.2165820642978 33.2994923857868 18.68020304568528 25.80372250423012 9 21.25211505922166 15.888324873096446 20.676818950930624 42.182741116751274 10-11 23.73942470389171 24.145516074450086 26.5989847715736 25.516074450084602 12-13 22.18274111675127 20.939086294416242 28.874788494077837 28.00338409475465 14-15 23.58714043993232 22.63959390862944 23.062605752961083 30.710659898477154 16-17 23.942470389170897 23.98477157360406 25.42301184433164 26.649746192893403 18-19 21.94585448392555 23.883248730964468 27.529610829103213 26.641285956006765 20-21 22.57191201353638 23.316412859560067 27.529610829103213 26.58206429780034 22-23 23.307952622673433 23.680203045685282 26.70896785109983 26.302876480541453 24-25 21.82741116751269 25.549915397631136 24.04399323181049 28.578680203045685 26-27 21.539763113367176 24.543147208121827 27.326565143824027 26.59052453468697 28-29 22.089678510998308 25.313028764805413 25.676818950930624 26.920473773265652 30-31 22.33502538071066 25.211505922165824 25.558375634517766 26.895093062605753 32-33 21.79357021996616 25.109983079526227 25.245346869712353 27.851099830795263 34-35 21.46362098138748 24.82233502538071 26.607445008460235 27.106598984771573 36-37 22.884940778341793 23.604060913705585 28.401015228426395 25.109983079526227 38-39 22.825719120135364 21.97969543147208 31.734348561759727 23.460236886632828 40-41 21.81895093062606 25.406091370558375 24.585448392554994 28.189509306260575 42-43 20.871404399323183 25.668358714043993 25.6175972927242 27.84263959390863 44-45 20.583756345177665 25.101522842639596 26.785109983079526 27.529610829103213 46-47 22.512690355329948 23.85786802030457 27.02199661590525 26.607445008460235 48-49 23.104906937394247 23.722504230118442 27.834179357022 25.338409475465312 50-51 24.56006768189509 22.58037225042301 29.23011844331641 23.629441624365484 52-53 21.489001692047378 24.22165820642978 27.18274111675127 27.106598984771573 54-55 21.700507614213198 25.0253807106599 27.081218274111674 26.192893401015226 56-57 22.038917089678513 24.238578680203045 26.56514382402707 27.157360406091367 58-59 21.04906937394247 25.033840947546533 26.57360406091371 27.343485617597292 60-61 20.812182741116754 25.45685279187817 26.446700507614214 27.284263959390863 62-63 20.871404399323183 25.719120135363788 26.818950930626055 26.59052453468697 64-65 21.25211505922166 25.549915397631136 25.761421319796955 27.436548223350254 66-67 19.568527918781726 26.20135363790186 23.56175972927242 30.668358714043993 68-69 19.790168372958796 25.721296217954144 24.84981808951688 29.638717319570183 70-71 20.076142131979697 25.736040609137056 23.790186125211505 30.397631133671744 72-73 19.534686971235192 25.6175972927242 23.21489001692047 31.632825719120135 74-75 20.372250423011845 26.25211505922166 24.99153976311337 28.384094754653134 76 20.829103214890015 25.668358714043993 26.39593908629442 27.106598984771573 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.5 16 0.5 17 0.0 18 0.5 19 1.0 20 2.5 21 4.0 22 5.5 23 11.5 24 16.5 25 17.0 26 18.5 27 21.0 28 29.5 29 37.0 30 48.0 31 64.5 32 87.0 33 104.0 34 112.5 35 150.5 36 171.5 37 163.0 38 189.0 39 248.0 40 289.5 41 297.5 42 297.0 43 303.5 44 306.0 45 317.0 46 332.0 47 320.0 48 295.0 49 275.0 50 268.0 51 252.5 52 207.0 53 171.5 54 166.0 55 152.5 56 145.0 57 128.0 58 105.0 59 103.0 60 92.0 61 88.0 62 93.0 63 90.0 64 92.0 65 90.5 66 88.5 67 93.0 68 91.5 69 95.5 70 92.0 71 83.0 72 76.5 73 63.5 74 50.5 75 44.0 76 44.5 77 38.5 78 25.0 79 18.0 80 18.5 81 12.5 82 5.0 83 4.0 84 3.5 85 3.0 86 2.5 87 2.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.008460236886632826 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 5910.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.44331641285956 #Duplication Level Percentage of deduplicated Percentage of total 1 98.65933310415951 97.12351945854483 2 1.1859745617050534 2.33502538071066 3 0.10312822275696115 0.3045685279187817 4 0.017188037126160193 0.0676818950930626 5 0.034376074252320386 0.1692047377326565 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0338409475465313 0.0 15 0.0 0.0 0.0 0.0338409475465313 0.0 16 0.0 0.0 0.0 0.0338409475465313 0.0 17 0.0 0.0 0.0 0.0338409475465313 0.0 18 0.0 0.0 0.0 0.0338409475465313 0.0 19 0.0 0.0 0.0 0.0338409475465313 0.0 20 0.0 0.0 0.0 0.0338409475465313 0.0 21 0.0 0.0 0.0 0.08460236886632826 0.0 22 0.0 0.0 0.0 0.10152284263959391 0.0 23 0.0 0.0 0.0 0.11844331641285956 0.0 24 0.0 0.0 0.0 0.18612521150592218 0.0 25 0.0 0.0 0.0 0.18612521150592218 0.0 26 0.0 0.0 0.0 0.18612521150592218 0.0 27 0.0 0.0 0.0 0.18612521150592218 0.0 28 0.0 0.0 0.0 0.21996615905245348 0.0 29 0.0 0.0 0.0 0.21996615905245348 0.0 30 0.0 0.0 0.0 0.25380710659898476 0.0 31 0.0 0.0 0.0 0.25380710659898476 0.0 32 0.0 0.0 0.0 0.25380710659898476 0.0 33 0.0 0.0 0.0 0.25380710659898476 0.0 34 0.0 0.0 0.0 0.30456852791878175 0.0 35 0.0 0.0 0.0 0.32148900169204736 0.0 36 0.0 0.0 0.0 0.338409475465313 0.0 37 0.0 0.0 0.0 0.37225042301184436 0.0 38 0.0 0.0 0.0 0.40609137055837563 0.0 39 0.0 0.0 0.0 0.4906937394247039 0.0 40 0.0 0.0 0.0 0.5583756345177665 0.0 41 0.0 0.0 0.0 0.6429780033840947 0.0 42 0.0 0.0 0.0 0.6937394247038917 0.0 43 0.0 0.0 0.0 0.7783417935702199 0.0 44 0.0 0.0 0.0 0.8629441624365483 0.0 45 0.0 0.0 0.0 0.9475465313028765 0.0 46 0.0 0.0 0.0 1.015228426395939 0.0 47 0.0 0.0 0.0 1.0998307952622672 0.0 48 0.0 0.0 0.0 1.2013536379018612 0.0 49 0.0 0.0 0.0 1.2859560067681894 0.0 50 0.0 0.0 0.0 1.3028764805414552 0.0 51 0.0 0.0 0.0 1.3367174280879865 0.0 52 0.0 0.0 0.0 1.4720812182741116 0.0 53 0.0 0.0 0.0 1.6582064297800339 0.0 54 0.0 0.0 0.0 1.7597292724196278 0.0 55 0.0 0.0 0.0 1.8612521150592216 0.0 56 0.0 0.0 0.0 1.9627749576988156 0.0 57 0.0 0.0 0.0 2.0642978003384096 0.0 58 0.0 0.0 0.0 2.1319796954314723 0.0 59 0.0 0.0 0.0 2.233502538071066 0.0 60 0.0 0.0 0.0 2.3181049069373945 0.0 61 0.0 0.0 0.0 2.4027072758037225 0.0 62 0.0 0.0 0.0 2.4196277495769882 0.0 63 0.0 0.0 0.0 2.487309644670051 0.0 64 0.0 0.0 0.0 2.571912013536379 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE