SRR1779706_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779706_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5910
Sequences flagged as poor quality	0
Sequence length	76
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	135	2.284263959390863	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	81	1.3705583756345179	RNA PCR Primer, Index 48 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	43	0.727580372250423	RNA PCR Primer, Index 48 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	26	0.4399323181049069	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	20	0.338409475465313	No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	12	0.20304568527918782	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	10	0.1692047377326565	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	9	0.15228426395939085	No Hit
GGGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTTAATTATG	8	0.1353637901861252	No Hit
ATGCTACAGGGATGAATATTATGGAGAAGTAGTCTAGTTTGAAGCTTAGG	7	0.11844331641285956	No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT	7	0.11844331641285956	Illumina PCR Primer Index 10 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT	7	0.11844331641285956	RNA PCR Primer, Index 15 (95% over 21bp)
GATTAGTAGTATGGGAGTGGGAGGGGAAAATAATGTGTTAGTTGGGGGGT	7	0.11844331641285956	No Hit
GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTG	6	0.10152284263959391	No Hit
ATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGCTTTATGA	6	0.10152284263959391	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT	6	0.10152284263959391	No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA	6	0.10152284263959391	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT	6	0.10152284263959391	No Hit
ACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCT	6	0.10152284263959391	RNA PCR Primer, Index 48 (96% over 25bp)
GTTAGGGGTCATGGGCTGGGTTTTACTATATGATAGGCATGTGATTGGTG	6	0.10152284263959391	No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC	6	0.10152284263959391	No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGA	6	0.10152284263959391	No Hit
CTATAAAAGCTATTGTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAG	6	0.10152284263959391	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	6	0.10152284263959391	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTTCT	15	0.002116507	70.0	12
AAGCGGG	15	0.002116507	70.0	64
TTTACAC	15	0.002116507	70.0	2
CTTTACA	20	0.006615848	52.500004	1
TTACACA	20	0.006615848	52.500004	3
CGTCAAT	55	5.638385E-6	38.18182	32
AATCTCG	55	5.638385E-6	38.18182	36
TTCGTCA	55	5.638385E-6	38.18182	30
GTCAATC	55	5.638385E-6	38.18182	33
CAATCTC	55	5.638385E-6	38.18182	35
TCAATCT	55	5.638385E-6	38.18182	34
TCGTCAA	55	5.638385E-6	38.18182	31
TTTCGTC	55	5.638385E-6	38.18182	29
AGACTTT	60	1.0246438E-5	35.0	25
CGAGACT	60	1.0246438E-5	35.0	23
GAGACTT	60	1.0246438E-5	35.0	24
ACTTTCG	65	1.7733984E-5	32.307693	27
TTGAAAA	65	1.7733984E-5	32.307693	58
CTTTCGT	65	1.7733984E-5	32.307693	28
ATCTCGT	70	2.9444078E-5	30.0	37

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content