##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779706_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5910
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02165820642978	34.0	34.0	34.0	31.0	34.0
2	33.256345177664976	34.0	34.0	34.0	33.0	34.0
3	33.306598984771576	34.0	34.0	34.0	33.0	34.0
4	33.39932318104907	34.0	34.0	34.0	34.0	34.0
5	33.32351945854484	34.0	34.0	34.0	34.0	34.0
6	36.62318104906937	38.0	37.0	38.0	34.0	38.0
7	36.66717428087986	38.0	37.0	38.0	34.0	38.0
8	36.23688663282572	38.0	37.0	38.0	34.0	38.0
9	36.126565143824024	38.0	37.0	38.0	33.0	38.0
10-11	36.19137055837563	38.0	37.0	38.0	34.0	38.0
12-13	36.18350253807107	38.0	37.0	38.0	33.5	38.0
14-15	36.25372250423012	38.0	37.0	38.0	34.0	38.0
16-17	36.07267343485618	38.0	36.5	38.0	33.0	38.0
18-19	36.1906937394247	38.0	37.0	38.0	34.0	38.0
20-21	36.24839255499154	38.0	37.0	38.0	34.0	38.0
22-23	35.97098138747885	38.0	36.0	38.0	32.0	38.0
24-25	36.01446700507614	38.0	36.0	38.0	32.0	38.0
26-27	35.810152284263964	38.0	36.0	38.0	31.5	38.0
28-29	35.813705583756345	38.0	36.0	38.0	31.5	38.0
30-31	35.77351945854484	38.0	36.0	38.0	31.0	38.0
32-33	35.616243654822334	38.0	36.0	38.0	31.0	38.0
34-35	35.40803722504231	38.0	36.0	38.0	31.0	38.0
36-37	35.535702199661586	38.0	36.0	38.0	31.0	38.0
38-39	35.5821489001692	38.0	36.0	38.0	31.0	38.0
40-41	35.755583756345175	38.0	36.0	38.0	31.0	38.0
42-43	35.813705583756345	38.0	36.0	38.0	31.0	38.0
44-45	35.716751269035534	38.0	36.0	38.0	31.0	38.0
46-47	35.818697123519456	38.0	36.0	38.0	31.5	38.0
48-49	35.78925549915398	38.0	36.0	38.0	31.0	38.0
50-51	35.76370558375635	38.0	36.0	38.0	31.0	38.0
52-53	35.669373942470386	38.0	36.0	38.0	31.0	38.0
54-55	35.694500846023686	38.0	36.0	38.0	31.0	38.0
56-57	35.542301184433164	38.0	36.0	38.0	31.0	38.0
58-59	35.516497461928935	38.0	36.0	38.0	31.0	38.0
60-61	35.593993231810494	38.0	36.0	38.0	31.0	38.0
62-63	35.50262267343486	38.0	36.0	38.0	30.0	38.0
64-65	35.50050761421319	38.0	36.0	38.0	31.0	38.0
66-67	35.469373942470384	38.0	36.0	38.0	31.0	38.0
68-69	35.05143824027073	38.0	36.0	38.0	27.0	38.0
70-71	34.41522842639594	37.0	34.5	38.0	24.0	38.0
72-73	34.11793570219966	38.0	34.5	38.0	23.0	38.0
74-75	33.98485617597292	38.0	35.0	38.0	22.0	38.0
76	32.783756345177665	37.0	32.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	0.0
23	2.0
24	7.0
25	6.0
26	14.0
27	20.0
28	45.0
29	74.0
30	125.0
31	187.0
32	255.0
33	471.0
34	678.0
35	947.0
36	1350.0
37	1727.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.95093062605753	21.489001692047378	12.284263959390863	27.275803722504232
2	20.812182741116754	20.473773265651438	37.055837563451774	21.658206429780034
3	21.04906937394247	26.091370558375637	31.40439932318105	21.455160744500844
4	16.26057529610829	19.72927241962775	35.465313028764804	28.544839255499156
5	14.111675126903553	37.64805414551608	34.331641285956	13.908629441624365
6	30.4906937394247	35.90524534686971	17.225042301184434	16.37901861252115
7	27.123519458544838	31.201353637901864	21.43824027072758	20.23688663282572
8	24.450084602368864	36.73434856175973	18.934010152284266	19.88155668358714
9	24.85617597292724	19.18781725888325	18.883248730964468	37.07275803722504
10-11	26.16751269035533	24.864636209813877	26.979695431472084	21.988155668358715
12-13	26.785109983079526	20.956006768189507	26.692047377326567	25.566835871404397
14-15	25.95600676818951	23.494077834179357	21.328257191201352	29.22165820642978
16-17	26.328257191201352	27.335025380710658	21.82741116751269	24.509306260575293
18-19	25.389170896785114	25.922165820642977	22.87648054145516	25.81218274111675
20-21	24.84771573604061	25.033840947546533	21.869712351945854	28.248730964467008
22-23	26.159052453468696	26.505922165820646	22.411167512690355	24.923857868020306
24-25	26.243654822335028	28.883248730964468	22.284263959390863	22.588832487309645
26-27	25.558375634517766	27.292724196277497	24.966159052453467	22.18274111675127
28-29	23.78172588832487	23.8917089678511	28.527918781725887	23.79864636209814
30-31	24.78849407783418	23.874788494077833	28.722504230118446	22.614213197969544
32-33	25.77834179357022	24.9746192893401	26.057529610829107	23.189509306260575
34-35	24.56006768189509	25.338409475465312	26.489001692047374	23.612521150592215
36-37	23.59560067681895	27.622673434856175	25.63451776649746	23.14720812182741
38-39	23.900169204737733	23.705583756345177	27.614213197969544	24.780033840947546
40-41	24.67005076142132	23.485617597292723	27.53807106598985	24.306260575296108
42-43	25.659898477157363	23.637901861252114	28.570219966159055	22.131979695431472
44-45	24.179357021996616	24.864636209813877	27.258883248730964	23.697123519458547
46-47	26.16751269035533	23.705583756345177	25.77834179357022	24.34856175972927
48-49	26.70896785109983	23.189509306260575	25.820642978003384	24.28087986463621
50-51	23.883248730964468	23.824027072758035	27.55499153976311	24.737732656514382
52-53	25.118443316412858	22.292724196277497	30.135363790186126	22.45346869712352
54-55	24.61082910321489	22.808798646362096	28.561759729272417	24.018612521150594
56-57	25.219966159052454	23.993231810490695	26.59052453468697	24.196277495769884
58-59	25.18612521150592	24.78849407783418	28.50253807106599	21.522842639593907
60-61	24.4585448392555	29.19627749576988	25.304568527918782	21.04060913705584
62-63	22.825719120135364	32.165820642978005	24.78849407783418	20.21996615905245
64-65	22.2419627749577	33.04568527918782	23.41793570219966	21.294416243654823
66-67	22.106598984771576	33.03722504230118	23.73942470389171	21.116751269035532
68-69	22.851099830795263	32.85109983079526	22.436548223350254	21.86125211505922
70-71	23.071065989847718	31.082910321489	24.526226734348562	21.31979695431472
72-73	21.97969543147208	29.991539763113366	24.585448392554994	23.44331641285956
74-75	22.978003384094755	29.13705583756345	24.28087986463621	23.604060913705585
76	22.84263959390863	28.40947546531303	24.703891708967852	24.04399323181049
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	1.5
19	5.0
20	8.5
21	9.0
22	12.5
23	19.0
24	16.0
25	10.0
26	20.5
27	33.0
28	39.5
29	44.0
30	63.0
31	86.0
32	94.5
33	99.0
34	110.0
35	153.0
36	180.5
37	176.0
38	211.5
39	289.5
40	362.0
41	407.5
42	423.0
43	381.5
44	346.0
45	315.5
46	279.0
47	271.5
48	249.0
49	229.5
50	225.0
51	200.5
52	157.0
53	135.0
54	132.0
55	122.5
56	108.5
57	96.0
58	88.0
59	91.0
60	81.0
61	70.0
62	72.0
63	78.0
64	75.0
65	76.0
66	85.0
67	84.0
68	81.5
69	79.0
70	72.5
71	66.0
72	75.5
73	75.5
74	58.0
75	50.0
76	46.5
77	46.0
78	35.5
79	22.0
80	21.0
81	15.5
82	8.5
83	6.0
84	4.5
85	4.5
86	3.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	5910.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.58375634517766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.89679874636221	62.656514382402705
2	11.55137676292814	17.461928934010153
3	3.8728453100514884	8.781725888324873
4	0.8282963957913588	2.504230118443316
5	0.31340944705618984	1.1844331641285957
6	0.24625027982986344	1.116751269035533
7	0.08954555630176853	0.47377326565143824
8	0.02238638907544213	0.1353637901861252
9	0.02238638907544213	0.15228426395939085
>10	0.11193194537721066	1.8781725888324874
>50	0.02238638907544213	1.3705583756345179
>100	0.02238638907544213	2.284263959390863
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	135	2.284263959390863	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	81	1.3705583756345179	RNA PCR Primer, Index 48 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	43	0.727580372250423	RNA PCR Primer, Index 48 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	26	0.4399323181049069	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	20	0.338409475465313	No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	12	0.20304568527918782	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	10	0.1692047377326565	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	9	0.15228426395939085	No Hit
GGGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTTAATTATG	8	0.1353637901861252	No Hit
ATGCTACAGGGATGAATATTATGGAGAAGTAGTCTAGTTTGAAGCTTAGG	7	0.11844331641285956	No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT	7	0.11844331641285956	Illumina PCR Primer Index 10 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT	7	0.11844331641285956	RNA PCR Primer, Index 15 (95% over 21bp)
GATTAGTAGTATGGGAGTGGGAGGGGAAAATAATGTGTTAGTTGGGGGGT	7	0.11844331641285956	No Hit
GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTG	6	0.10152284263959391	No Hit
ATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGCTTTATGA	6	0.10152284263959391	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT	6	0.10152284263959391	No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA	6	0.10152284263959391	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT	6	0.10152284263959391	No Hit
ACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCT	6	0.10152284263959391	RNA PCR Primer, Index 48 (96% over 25bp)
GTTAGGGGTCATGGGCTGGGTTTTACTATATGATAGGCATGTGATTGGTG	6	0.10152284263959391	No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC	6	0.10152284263959391	No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGA	6	0.10152284263959391	No Hit
CTATAAAAGCTATTGTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAG	6	0.10152284263959391	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	6	0.10152284263959391	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.01692047377326565	0.0
20	0.0	0.0	0.0	0.01692047377326565	0.0
21	0.0	0.0	0.0	0.0338409475465313	0.0
22	0.0	0.0	0.0	0.0338409475465313	0.0
23	0.0	0.0	0.0	0.0338409475465313	0.0
24	0.0	0.0	0.0	0.0338409475465313	0.0
25	0.0	0.0	0.0	0.0338409475465313	0.0
26	0.0	0.0	0.0	0.0338409475465313	0.0
27	0.0	0.0	0.0	0.0338409475465313	0.0
28	0.0	0.0	0.0	0.0676818950930626	0.0
29	0.0	0.0	0.0	0.10152284263959391	0.0
30	0.0	0.0	0.0	0.11844331641285956	0.0
31	0.0	0.0	0.0	0.1353637901861252	0.0
32	0.0	0.0	0.0	0.15228426395939088	0.0
33	0.0	0.0	0.0	0.1692047377326565	0.0
34	0.0	0.0	0.0	0.20304568527918782	0.0
35	0.0	0.0	0.0	0.25380710659898476	0.0
36	0.0	0.0	0.0	0.338409475465313	0.0
37	0.0	0.0	0.0	0.43993231810490696	0.0
38	0.0	0.0	0.0	0.5245346869712352	0.0
39	0.0	0.0	0.0	0.676818950930626	0.0
40	0.0	0.0	0.0	0.7783417935702199	0.0
41	0.0	0.0	0.0	0.8629441624365483	0.0
42	0.0	0.0	0.0	0.9475465313028765	0.0
43	0.0	0.0	0.0	0.9983079526226735	0.0
44	0.0	0.0	0.0	1.0998307952622672	0.0
45	0.0	0.0	0.0	1.2351945854483926	0.0
46	0.0	0.0	0.0	1.2859560067681894	0.0
47	0.0	0.0	0.0	1.3705583756345177	0.0
48	0.0	0.0	0.0	1.5228426395939085	0.0
49	0.0	0.0	0.0	1.6751269035532994	0.0
50	0.0	0.0	0.0	1.6920473773265652	0.0
51	0.0	0.0	0.0	1.7597292724196278	0.0
52	0.0	0.0	0.0	1.8781725888324874	0.0
53	0.0	0.0	0.0	2.047377326565144	0.0
54	0.0	0.0	0.0	2.1489001692047376	0.0
55	0.0	0.0	0.0	2.3011844331641287	0.0
56	0.0	0.0	0.0	2.470389170896785	0.0
57	0.0	0.0	0.0	2.6226734348561758	0.0
58	0.0	0.0	0.0	2.72419627749577	0.0
59	0.0	0.0	0.0	2.808798646362098	0.0
60	0.0	0.0	0.0	2.9272419627749575	0.0
61	0.0	0.0	0.0	3.1472081218274113	0.0
62	0.0	0.0	0.0	3.299492385786802	0.0
63	0.0	0.0	0.0	3.451776649746193	0.0
64	0.0	0.0	0.0	3.6379018612521152	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTTCT	15	0.002116507	70.0	12
AAGCGGG	15	0.002116507	70.0	64
TTTACAC	15	0.002116507	70.0	2
CTTTACA	20	0.006615848	52.500004	1
TTACACA	20	0.006615848	52.500004	3
CGTCAAT	55	5.638385E-6	38.18182	32
AATCTCG	55	5.638385E-6	38.18182	36
TTCGTCA	55	5.638385E-6	38.18182	30
GTCAATC	55	5.638385E-6	38.18182	33
CAATCTC	55	5.638385E-6	38.18182	35
TCAATCT	55	5.638385E-6	38.18182	34
TCGTCAA	55	5.638385E-6	38.18182	31
TTTCGTC	55	5.638385E-6	38.18182	29
AGACTTT	60	1.0246438E-5	35.0	25
CGAGACT	60	1.0246438E-5	35.0	23
GAGACTT	60	1.0246438E-5	35.0	24
ACTTTCG	65	1.7733984E-5	32.307693	27
TTGAAAA	65	1.7733984E-5	32.307693	58
CTTTCGT	65	1.7733984E-5	32.307693	28
ATCTCGT	70	2.9444078E-5	30.0	37
>>END_MODULE