##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779705_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15962
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43565969176795	34.0	32.0	34.0	23.0	34.0
2	29.250156621977197	34.0	27.0	34.0	12.0	34.0
3	28.399323393058513	34.0	23.0	34.0	11.0	34.0
4	31.286367623104873	34.0	32.0	34.0	23.0	34.0
5	30.70899636637013	34.0	32.0	34.0	21.0	34.0
6	33.41053752662574	37.0	34.0	38.0	21.0	38.0
7	34.43854153614835	38.0	34.0	38.0	26.0	38.0
8	33.72177671970931	38.0	34.0	38.0	21.0	38.0
9	35.08407467735873	38.0	36.0	38.0	27.0	38.0
10-11	34.99808921187821	38.0	36.0	38.0	26.5	38.0
12-13	33.69198721964666	37.5	32.5	38.0	19.5	38.0
14-15	34.027534143591026	37.5	34.5	38.0	23.5	38.0
16-17	33.57827966420248	37.5	32.5	38.0	19.0	38.0
18-19	34.18230798145596	38.0	34.0	38.0	23.5	38.0
20-21	33.22378148101741	37.5	31.0	38.0	18.5	38.0
22-23	33.07470868312242	37.5	31.5	38.0	19.0	38.0
24-25	34.66191580002506	37.5	35.0	38.0	25.5	38.0
26-27	32.790690389675476	37.0	30.5	38.0	17.0	38.0
28-29	35.29542037338679	38.0	36.5	38.0	29.0	38.0
30-31	35.67767197093096	38.0	37.0	38.0	31.0	38.0
32-33	35.74220022553565	38.0	37.0	38.0	31.0	38.0
34-35	35.78411226663325	38.0	37.0	38.0	31.5	38.0
36-37	33.5203921814309	38.0	33.0	38.0	18.5	38.0
38-39	31.341341937100616	36.5	27.0	38.0	11.0	38.0
40-41	33.11167146974063	37.0	32.5	38.0	17.0	38.0
42-43	34.14261997243453	37.5	35.0	38.0	19.0	38.0
44-45	35.77189575241198	38.0	37.0	38.0	32.0	38.0
46-47	34.14321513594788	38.0	34.0	38.0	22.0	38.0
48-49	33.6327527878712	37.5	33.5	38.0	19.0	38.0
50-51	31.19308357348703	36.5	26.5	38.0	11.0	38.0
52-53	34.982959528881096	37.5	35.5	38.0	28.5	38.0
54-55	34.02333667460218	37.5	34.5	38.0	19.0	38.0
56-57	34.42876832477133	38.0	35.5	38.0	21.0	38.0
58-59	35.54971181556196	38.0	37.0	38.0	31.0	38.0
60-61	35.91720962285428	38.0	37.0	38.0	33.5	38.0
62-63	35.79880340809423	38.0	37.0	38.0	32.5	38.0
64-65	35.54864678611703	38.0	37.0	38.0	30.5	38.0
66-67	33.452230296955264	38.0	34.0	38.0	21.0	38.0
68-69	33.426888861044986	37.5	34.0	38.0	16.5	38.0
70-71	32.82057386292445	37.0	32.5	38.0	16.0	38.0
72-73	31.599517604310236	37.0	29.0	38.0	11.0	38.0
74-75	34.130278160631505	37.5	35.0	38.0	24.0	38.0
76	34.813056008019046	37.0	36.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	12.0
14	34.0
15	72.0
16	92.0
17	116.0
18	136.0
19	139.0
20	113.0
21	96.0
22	107.0
23	115.0
24	89.0
25	114.0
26	122.0
27	161.0
28	216.0
29	235.0
30	337.0
31	489.0
32	764.0
33	1285.0
34	1918.0
35	3095.0
36	3881.0
37	2223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.77784738754542	23.280290690389677	12.70517479012655	31.23668713193835
2	18.500187946372634	19.264503195088334	38.12178924946748	24.113519609071545
3	18.813431900764314	19.527628116777347	33.65493045984213	28.004009522616215
4	13.024683623606064	18.224533266507954	35.13970680365869	33.61107630622729
5	12.936975316376396	32.07618092970806	38.17190828217015	16.814935471745397
6	26.061897005387795	34.062147600551306	17.86743515850144	22.008520235559452
7	25.61082571106378	29.137952637514097	21.93334168650545	23.317879964916678
8	21.651422127552937	33.68625485528129	18.876080691642652	25.78624232552312
9	22.183936850018796	17.12191454704924	21.300588898634256	39.39355970429771
10-11	23.840997368750784	25.61395815060769	25.996115774965546	24.54892870567598
12-13	22.876205989224406	22.537902518481392	28.057260994862798	26.5286304974314
14-15	21.297456459090338	23.878586643277785	23.781481017416365	31.042475880215513
16-17	21.742262874326528	26.337551685252475	27.208369878461347	24.711815561959654
18-19	20.79626613206365	26.65392807918807	27.330535020674102	25.219270768074175
20-21	21.57937601804285	25.466733492043602	27.77534143591029	25.17854905400326
22-23	21.883222653802783	25.087708307229672	28.298458839744395	24.730610199223154
24-25	20.301340684124796	27.255356471620097	26.061897005387795	26.381405838867312
26-27	20.727352462097482	25.78624232552312	28.423756421501068	25.062648790878335
28-29	21.382032326776095	27.255356471620097	27.09873449442426	24.263876707179552
30-31	20.746147099360982	27.305475504322768	26.152737752161382	25.795639644154868
32-33	20.320135321388296	27.19584012028568	27.15511840621476	25.328906152111262
34-35	21.34444305224909	26.606941486029317	27.349329657937606	24.699285803783987
36-37	20.780603934344064	24.833980704172408	30.043227665706052	24.34218769577747
38-39	21.1408344818945	24.389174288936225	31.430898383661194	23.039092845508083
40-41	21.57937601804285	24.514471870692894	26.0806916426513	27.825460468612956
42-43	19.424257611828093	25.24119784488159	26.882596165893997	28.45194837739632
44-45	20.63024683623606	26.403332915674728	27.19584012028568	25.770580127803534
46-47	21.14709936098233	25.144092219020177	28.483272772835484	25.225535647162012
48-49	21.024934218769577	26.306227289813304	27.884976819947376	24.78386167146974
50-51	21.07192081192833	24.11665204861546	30.635258739506327	24.176168399949884
52-53	20.921563713820323	25.60769327151986	27.791003633629867	25.679739381029947
54-55	20.674100989850896	26.187194587144468	28.022804159879716	25.115900263124924
56-57	20.790001252975816	25.23806540533768	28.45508081694023	25.51685252474627
58-59	20.21049993735121	26.346949003884223	28.41749154241323	25.025059516351334
60-61	20.301340684124796	25.88021551184062	27.84738754542037	25.97105625861421
62-63	19.449317128179427	26.544292695150983	27.935095852650043	26.071294324019544
64-65	20.244956772334294	27.10813181305601	26.541160255607068	26.105751159002633
66-67	19.10161633880466	24.595915298834733	25.68287182057386	30.61959654178674
68-69	19.28571428571429	25.159774436090228	26.42857142857143	29.12593984962406
70-71	19.521363237689513	24.752537276030573	25.285051998496428	30.441047487783486
72-73	18.991980954767573	23.339807041724097	24.968675604560833	32.6995363989475
74-75	19.822077433905523	26.18092970805663	26.888861044981834	27.10813181305601
76	20.68036586893873	26.331286806164638	26.976569352211506	26.01177797268513
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.0
16	0.5
17	1.0
18	3.0
19	5.0
20	8.5
21	12.0
22	14.0
23	21.0
24	30.0
25	34.0
26	40.5
27	65.0
28	101.5
29	120.0
30	143.5
31	191.5
32	255.5
33	295.0
34	339.0
35	413.0
36	486.5
37	530.0
38	609.5
39	776.5
40	882.5
41	933.0
42	965.0
43	951.5
44	947.0
45	964.5
46	973.0
47	934.0
48	868.5
49	792.0
50	742.0
51	699.0
52	614.0
53	531.5
54	491.0
55	442.0
56	372.0
57	307.0
58	263.0
59	256.0
60	231.5
61	196.0
62	178.0
63	168.5
64	160.0
65	140.0
66	119.0
67	119.0
68	134.0
69	124.5
70	105.0
71	110.0
72	113.0
73	102.0
74	85.5
75	83.0
76	73.0
77	61.0
78	48.5
79	38.0
80	27.0
81	18.0
82	17.0
83	14.0
84	12.5
85	9.0
86	5.0
87	3.0
88	4.0
89	3.5
90	1.0
91	0.0
92	0.0
93	1.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.01252975817566721
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	15962.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.06803658689387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.54743771782626	94.68738253351711
2	2.0717697173099263	4.022052374389174
3	0.2646185620240093	0.7705801278035335
4	0.07099522395766103	0.2756546798646786
5	0.032270556344391375	0.15662197719584012
6	0.006454111268878275	0.03758927452700163
7	0.0	0.0
8	0.006454111268878275	0.05011903270266884
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.006264879087833605	0.0
5	0.0	0.0	0.0	0.006264879087833605	0.0
6	0.0	0.0	0.0	0.006264879087833605	0.0
7	0.0	0.0	0.0	0.006264879087833605	0.0
8	0.0	0.0	0.0	0.006264879087833605	0.0
9	0.0	0.0	0.0	0.006264879087833605	0.0
10	0.0	0.0	0.0	0.006264879087833605	0.0
11	0.0	0.0	0.0	0.006264879087833605	0.0
12	0.0	0.0	0.0	0.006264879087833605	0.0
13	0.0	0.0	0.0	0.006264879087833605	0.0
14	0.0	0.0	0.0	0.006264879087833605	0.0
15	0.0	0.0	0.0	0.006264879087833605	0.0
16	0.0	0.0	0.0	0.006264879087833605	0.0
17	0.0	0.0	0.0	0.006264879087833605	0.0
18	0.0	0.0	0.0	0.006264879087833605	0.0
19	0.0	0.0	0.0	0.01252975817566721	0.0
20	0.0	0.0	0.0	0.01252975817566721	0.0
21	0.0	0.0	0.0	0.01252975817566721	0.0
22	0.0	0.0	0.0	0.03758927452700163	0.0
23	0.0	0.0	0.0	0.04385415361483523	0.0
24	0.0	0.0	0.0	0.05011903270266884	0.0
25	0.0	0.0	0.0	0.05011903270266884	0.0
26	0.0	0.0	0.0	0.05011903270266884	0.0
27	0.0	0.0	0.0	0.05011903270266884	0.0
28	0.0	0.0	0.0	0.05011903270266884	0.0
29	0.0	0.0	0.0	0.05011903270266884	0.0
30	0.0	0.0	0.0	0.05011903270266884	0.0
31	0.0	0.0	0.0	0.05011903270266884	0.0
32	0.0	0.0	0.0	0.05011903270266884	0.0
33	0.0	0.0	0.0	0.05638391179050244	0.0
34	0.0	0.0	0.0	0.06891366996616966	0.0
35	0.0	0.0	0.0	0.08144342814183686	0.0
36	0.0	0.0	0.0	0.11276782358100489	0.0
37	0.0	0.0	0.0	0.1440922190201729	0.0
38	0.0	0.0	0.0	0.19421125172284176	0.0
39	0.0	0.0	0.0	0.24433028442551058	0.0
40	0.0	0.0	0.0	0.31324395439168023	0.0
41	0.0	0.0	0.0	0.3884225034456835	0.0
42	0.0	0.0	0.0	0.47613081067535395	0.0
43	0.0	0.0	0.0	0.6578123042225285	0.0
44	0.0	0.0	0.0	0.7893747650670342	0.0
45	0.0	0.0	0.0	0.8770830722967047	0.0
46	0.0	0.0	0.0	1.0211752913168777	0.0
47	0.0	0.0	0.0	1.190327026688385	0.0
48	0.0	0.0	0.0	1.3532138829720586	0.0
49	0.0	0.0	0.0	1.4346573111138956	0.0
50	0.0	0.0	0.0	1.6351334419245709	0.0
51	0.0	0.0	0.0	1.8731988472622478	0.0
52	0.0	0.0	0.0	2.0799398571607566	0.0
53	0.0	0.0	0.0	2.3493296579376017	0.0
54	0.0	0.0	0.0	2.662573612329282	0.0
55	0.0	0.0	0.0	2.931963413106127	0.0
56	0.0	0.0	0.0	3.126174664828969	0.0
57	0.0	0.0	0.0	3.3266507956396443	0.0
58	0.0	0.0	0.0	3.439418619220649	0.0
59	0.0	0.0	0.0	3.577245959152988	0.0
60	0.0	0.0	0.0	3.6962786618218266	0.0
61	0.0	0.0	0.0	3.8716952762811676	0.0
62	0.0	0.0	0.0	3.9406089462473375	0.0
63	0.0	0.0	0.0	4.090966044355344	0.0
64	0.0	0.0	0.0	4.247588021551184	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTACGA	15	0.0021847868	70.00001	21
TTCAATT	15	0.0021847868	70.00001	52
TGTTTAT	15	0.0021847868	70.00001	58
>>END_MODULE