FastQCFastQC Report
Wed 25 May 2016
SRR1779705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779705_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15962
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1430.8958777095602055No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT850.5325147224658564TruSeq Adapter, Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC480.30071419621601303No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC340.21300588898634257No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC260.16288685628367372No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG170.10650294449317128No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGGG150.002184786870.000018
TCTAGAG200.006827636652.53
ATTGAAG200.006827636652.557
TCGTATG905.691105E-627.22222140
CTCGTAT905.691105E-627.22222139
TGAAAAA1056.1016726E-726.66666659
GCACGTA808.1566235E-526.2531
ACGAGCA808.1566235E-526.2527
GACGAGC808.1566235E-526.2526
GTATGCC958.663241E-625.78947442
TGCCGTC958.663241E-625.78947445
TATGCCG958.663241E-625.78947443
CCGTCTT958.663241E-625.78947447
GAGCACG958.663241E-625.78947429
ATGCCGT958.663241E-625.78947444
CGTATGC958.663241E-625.78947441
CGAGCAC958.663241E-625.78947428
GCCGTCT958.663241E-625.78947446
CGTCTTC958.663241E-625.78947448
TATCTCG700.001174321425.036
Produced by FastQC (version 0.11.5)