##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779705_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15962
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92757799774464	34.0	34.0	34.0	31.0	34.0
2	33.24138579125423	34.0	34.0	34.0	32.0	34.0
3	33.29845883974439	34.0	34.0	34.0	33.0	34.0
4	33.353652424508205	34.0	34.0	34.0	33.0	34.0
5	33.36555569477509	34.0	34.0	34.0	33.0	34.0
6	36.569101616338806	38.0	37.0	38.0	34.0	38.0
7	36.57524119784488	38.0	37.0	38.0	34.0	38.0
8	36.21388297205864	38.0	37.0	38.0	34.0	38.0
9	35.988034080942235	38.0	36.0	38.0	32.0	38.0
10-11	36.08438792131312	38.0	36.5	38.0	33.0	38.0
12-13	36.148195714822705	38.0	37.0	38.0	34.0	38.0
14-15	36.182151359478766	38.0	37.0	38.0	34.0	38.0
16-17	36.099642901892	38.0	36.5	38.0	33.0	38.0
18-19	36.151328154366624	38.0	36.5	38.0	33.5	38.0
20-21	36.168807167021676	38.0	37.0	38.0	34.0	38.0
22-23	35.978793384287684	38.0	36.0	38.0	32.0	38.0
24-25	35.983523367999	38.0	36.0	38.0	32.0	38.0
26-27	35.80315749906027	38.0	36.0	38.0	31.5	38.0
28-29	35.73903646159629	38.0	36.0	38.0	31.5	38.0
30-31	35.67651296829971	38.0	36.0	38.0	31.0	38.0
32-33	35.721463475754916	38.0	36.0	38.0	31.0	38.0
34-35	35.644499436160885	38.0	36.0	38.0	31.0	38.0
36-37	35.711533642400695	38.0	36.0	38.0	31.0	38.0
38-39	35.83194461846887	38.0	36.0	38.0	32.0	38.0
40-41	35.823455707304845	38.0	36.0	38.0	32.0	38.0
42-43	35.737156997869945	38.0	36.0	38.0	31.0	38.0
44-45	35.76747901265506	38.0	36.0	38.0	31.5	38.0
46-47	35.804473123668714	38.0	36.0	38.0	32.0	38.0
48-49	35.72519107881218	38.0	36.0	38.0	31.0	38.0
50-51	35.7952637514096	38.0	36.0	38.0	31.5	38.0
52-53	35.69778223280291	38.0	36.0	38.0	31.0	38.0
54-55	35.709027690765566	38.0	36.0	38.0	31.0	38.0
56-57	35.69571482270392	38.0	36.0	38.0	31.0	38.0
58-59	35.6995363989475	38.0	36.0	38.0	31.0	38.0
60-61	35.69800150357098	38.0	36.0	38.0	31.0	38.0
62-63	35.5746147099361	38.0	36.0	38.0	31.0	38.0
64-65	35.55785615837614	38.0	36.0	38.0	31.0	38.0
66-67	35.58072296704674	38.0	36.0	38.0	31.0	38.0
68-69	35.389894750031324	38.0	36.0	38.0	30.5	38.0
70-71	35.23449442425761	38.0	36.0	38.0	28.0	38.0
72-73	35.19443052249092	38.0	36.0	38.0	27.5	38.0
74-75	35.24220022553565	38.0	36.0	38.0	27.5	38.0
76	34.020924696153365	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	6.0
24	5.0
25	17.0
26	41.0
27	65.0
28	98.0
29	150.0
30	300.0
31	412.0
32	761.0
33	1072.0
34	1753.0
35	2571.0
36	3779.0
37	4931.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.525122165142214	23.956897631875705	13.218894875328907	23.299085327653177
2	20.755544417992734	22.503445683498306	35.3652424508207	21.37576744768826
3	19.9849642901892	25.498057887482773	30.99862172660068	23.518356095727352
4	16.420248089211878	18.675604560831978	39.30585139706804	25.59829595288811
5	15.561959654178676	37.48903646159629	34.168650545044486	12.780353339180554
6	31.91955895251222	35.910286931462224	17.942613707555445	14.227540408470116
7	28.924946748527752	30.32827966420248	23.173787745896504	17.572985841373264
8	24.971808044104748	36.16088209497556	19.790753038466356	19.076556822453327
9	26.29369753163764	18.694399198095475	21.682746522992105	33.32915674727478
10-11	26.826212254103492	25.66720962285428	26.917053000877083	20.589525122165142
12-13	26.992231549931084	22.819822077433905	26.133943114897885	24.054003257737126
14-15	25.316376393935595	24.339055256233554	22.860543791504824	27.484024558326027
16-17	25.538779601553692	27.81293071043729	24.48001503570981	22.16827465229921
18-19	24.664828968800904	26.35947876205989	26.1182809171783	22.857411351960906
20-21	23.781481017416365	26.841874451823077	25.952261621350708	23.42438290940985
22-23	24.758802155118406	27.54354090966044	25.826964039594035	21.870692895627116
24-25	25.181681493547174	27.496554316501694	25.620223029695527	21.70154116025561
26-27	24.126049367247212	28.395564465605815	26.41899511339431	21.059391053752663
28-29	25.350833228918685	26.757298584137324	26.76669590276908	21.125172284174916
30-31	25.10650294449317	26.82307981455958	26.87946372635008	21.19095351459717
32-33	24.364114772584887	27.806665831349452	26.240446059391054	21.588773336674603
34-35	25.601428392432023	26.32188948753289	26.995363989475003	21.08131813056008
36-37	24.611577496554315	26.747901265505575	26.957774714948002	21.682746522992105
38-39	23.703170028818445	26.788622979576495	27.33366746021802	22.174539531387044
40-41	24.032076180929707	25.98985089587771	27.913168775842628	22.064904147349957
42-43	24.82458338554066	25.789374765067034	28.188823455707308	21.197218393685002
44-45	23.759553940608946	26.72910662824208	27.596792381907033	21.91454704924195
46-47	25.037589274527	25.366495426638263	27.011026187194588	22.584889111640145
48-49	24.7024182433279	24.9812053627365	27.603057260994863	22.713319132940736
50-51	23.98195714822704	25.642150106502942	27.487156997869945	22.888735747400073
52-53	23.52148853527127	25.460468612955772	29.2695150983586	21.748527753414358
54-55	23.52148853527127	25.23493296579376	28.953138704423004	22.290439794511965
56-57	24.74627239694274	24.72434532013532	27.794136073173785	22.73524620974815
58-59	23.869189324646033	25.98045357724596	28.621100112767824	21.529256985340183
60-61	23.83473248966295	27.80353339180554	27.15511840621476	21.20661571231675
62-63	22.42513469490039	29.02518481393309	26.826212254103492	21.723468237063024
64-65	22.437664453076057	29.689261997243456	26.12767823581005	21.745395313870443
66-67	22.750908407467733	29.100363362987093	26.5286304974314	21.62009773211377
68-69	22.46272396942739	29.77697030447312	25.952261621350708	21.808044104748777
70-71	22.89186818694399	29.16301215386543	25.98671845633379	21.958401202856784
72-73	22.71958401202857	27.816063149981208	26.278035333918055	23.18631750407217
74-75	22.920060142839244	27.734619721839373	26.619471244204984	22.725848891116403
76	22.99837113143716	27.922566094474377	25.836361358225783	23.242701415862673
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	1.0
16	0.0
17	0.0
18	3.0
19	6.0
20	8.0
21	10.0
22	13.5
23	23.5
24	46.5
25	63.0
26	77.0
27	101.0
28	140.5
29	170.0
30	198.5
31	227.5
32	277.0
33	326.0
34	396.0
35	458.0
36	517.0
37	584.0
38	648.0
39	764.5
40	910.0
41	989.5
42	976.0
43	1049.5
44	1084.5
45	1013.0
46	980.0
47	892.5
48	793.5
49	740.5
50	699.0
51	646.5
52	531.5
53	414.5
54	360.0
55	356.0
56	314.0
57	255.5
58	235.0
59	210.5
60	180.5
61	157.0
62	139.0
63	138.0
64	138.0
65	132.0
66	127.5
67	130.0
68	133.5
69	116.5
70	100.5
71	105.0
72	105.0
73	101.5
74	89.5
75	81.0
76	78.5
77	73.5
78	63.0
79	55.0
80	45.0
81	25.5
82	17.0
83	18.0
84	14.5
85	7.5
86	2.5
87	1.0
88	2.0
89	1.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	15962.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.00613958150608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.58073054526204	55.08708181932088
2	14.425622022233986	20.486154617215888
3	4.482089288865361	9.547675729858414
4	1.738133050997	4.93672472121288
5	0.7323098641256397	2.599924821450946
6	0.4411505205576143	1.8794637263500815
7	0.2823363331568731	1.4033329156747276
8	0.09705311452267514	0.5513093597293572
9	0.044115052055761425	0.28191955895251225
>10	0.15881418740074113	1.7980202982082447
>50	0.008823010411152285	0.5325147224658564
>100	0.008823010411152285	0.8958777095602055
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	143	0.8958777095602055	No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT	85	0.5325147224658564	TruSeq Adapter, Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	48	0.30071419621601303	No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	34	0.21300588898634257	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	26	0.16288685628367372	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	17	0.10650294449317128	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.006264879087833605	0.0
5	0.0	0.0	0.0	0.006264879087833605	0.0
6	0.0	0.0	0.0	0.006264879087833605	0.0
7	0.0	0.0	0.0	0.006264879087833605	0.0
8	0.0	0.0	0.0	0.006264879087833605	0.0
9	0.0	0.0	0.0	0.006264879087833605	0.0
10	0.0	0.0	0.0	0.006264879087833605	0.0
11	0.0	0.0	0.0	0.006264879087833605	0.0
12	0.0	0.0	0.0	0.006264879087833605	0.0
13	0.0	0.0	0.0	0.006264879087833605	0.0
14	0.0	0.0	0.0	0.006264879087833605	0.0
15	0.0	0.0	0.0	0.006264879087833605	0.0
16	0.0	0.0	0.0	0.006264879087833605	0.0
17	0.0	0.0	0.0	0.006264879087833605	0.0
18	0.0	0.0	0.0	0.006264879087833605	0.0
19	0.0	0.0	0.0	0.018794637263500814	0.0
20	0.0	0.0	0.0	0.018794637263500814	0.0
21	0.0	0.0	0.0	0.018794637263500814	0.0
22	0.0	0.0	0.0	0.018794637263500814	0.0
23	0.0	0.0	0.0	0.02505951635133442	0.0
24	0.0	0.0	0.0	0.02505951635133442	0.0
25	0.0	0.0	0.0	0.02505951635133442	0.0
26	0.0	0.0	0.0	0.02505951635133442	0.0
27	0.0	0.0	0.0	0.02505951635133442	0.0
28	0.0	0.0	0.0	0.02505951635133442	0.0
29	0.0	0.0	0.0	0.02505951635133442	0.0
30	0.0	0.0	0.0	0.02505951635133442	0.0
31	0.0	0.0	0.0	0.02505951635133442	0.0
32	0.0	0.0	0.0	0.02505951635133442	0.0
33	0.0	0.0	0.0	0.03758927452700163	0.0
34	0.0	0.0	0.0	0.05638391179050244	0.0
35	0.0	0.0	0.0	0.07517854905400326	0.0
36	0.0	0.0	0.0	0.1252975817566721	0.0
37	0.0	0.0	0.0	0.22553564716200977	0.0
38	0.0	0.0	0.0	0.2819195589525122	0.0
39	0.0	0.0	0.0	0.3758927452700163	0.0
40	0.0	0.0	0.0	0.46360105249968675	0.0
41	0.0	0.0	0.0	0.5513093597293572	0.0
42	0.0	0.0	0.0	0.6390176669590277	0.0
43	0.0	0.0	0.0	0.8457586768575367	0.0
44	0.0	0.0	0.0	0.9898508958777096	0.0
45	0.0	0.0	0.0	1.1527377521613833	0.0
46	0.0	0.0	0.0	1.3720085202355594	0.0
47	0.0	0.0	0.0	1.572484651046235	0.0
48	0.0	0.0	0.0	1.854404209998747	0.0
49	0.0	0.0	0.0	2.0047613081067537	0.0
50	0.0	0.0	0.0	2.2804159879714323	0.0
51	0.0	0.0	0.0	2.53727603057261	0.0
52	0.0	0.0	0.0	2.769076556822453	0.0
53	0.0	0.0	0.0	3.057260994862799	0.0
54	0.0	0.0	0.0	3.376769828342313	0.0
55	0.0	0.0	0.0	3.7150732990853275	0.0
56	0.0	0.0	0.0	3.9844630998621726	0.0
57	0.0	0.0	0.0	4.2789124169903525	0.0
58	0.0	0.0	0.0	4.485653426888861	0.0
59	0.0	0.0	0.0	4.755043227665706	0.0
60	0.0	0.0	0.0	5.011903270266884	0.0
61	0.0	0.0	0.0	5.293822829219396	0.0
62	0.0	0.0	0.0	5.538153113644906	0.0
63	0.0	0.0	0.0	5.832602430773086	0.0
64	0.0	0.0	0.0	6.101992231549931	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGGGG	15	0.0021847868	70.00001	8
TCTAGAG	20	0.0068276366	52.5	3
ATTGAAG	20	0.0068276366	52.5	57
TCGTATG	90	5.691105E-6	27.222221	40
CTCGTAT	90	5.691105E-6	27.222221	39
TGAAAAA	105	6.1016726E-7	26.666666	59
GCACGTA	80	8.1566235E-5	26.25	31
ACGAGCA	80	8.1566235E-5	26.25	27
GACGAGC	80	8.1566235E-5	26.25	26
GTATGCC	95	8.663241E-6	25.789474	42
TGCCGTC	95	8.663241E-6	25.789474	45
TATGCCG	95	8.663241E-6	25.789474	43
CCGTCTT	95	8.663241E-6	25.789474	47
GAGCACG	95	8.663241E-6	25.789474	29
ATGCCGT	95	8.663241E-6	25.789474	44
CGTATGC	95	8.663241E-6	25.789474	41
CGAGCAC	95	8.663241E-6	25.789474	28
GCCGTCT	95	8.663241E-6	25.789474	46
CGTCTTC	95	8.663241E-6	25.789474	48
TATCTCG	70	0.0011743214	25.0	36
>>END_MODULE