##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779704_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6994
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.799256505576206	33.0	23.0	34.0	12.0	34.0
2	27.860737775235915	33.0	23.0	34.0	12.0	34.0
3	26.63254217901058	32.0	21.0	34.0	11.0	34.0
4	29.124964255075778	33.0	23.0	34.0	12.0	34.0
5	28.319702602230482	33.0	23.0	34.0	11.0	34.0
6	30.756219616814413	36.0	27.0	38.0	11.0	38.0
7	31.763940520446095	36.0	27.0	38.0	12.0	38.0
8	31.437231913068345	36.0	27.0	38.0	11.0	38.0
9	32.9907063197026	37.0	33.0	38.0	21.0	38.0
10-11	32.543966256791535	37.0	31.5	38.0	16.0	38.0
12-13	31.36316843008293	35.5	28.5	38.0	16.0	38.0
14-15	31.57749499571061	36.0	29.0	38.0	11.5	38.0
16-17	31.12360594795539	35.5	27.5	38.0	16.0	38.0
18-19	31.70646268229911	36.0	29.0	38.0	11.5	38.0
20-21	30.581927366313984	35.0	26.5	37.5	16.0	38.0
22-23	30.55225907921075	35.5	26.0	37.5	16.0	38.0
24-25	32.061338289962826	36.5	29.0	38.0	16.5	38.0
26-27	30.28903345724907	35.0	24.5	38.0	11.0	38.0
28-29	32.919002001715754	37.0	32.5	38.0	21.0	38.0
30-31	33.61273949099228	37.0	34.0	38.0	21.5	38.0
32-33	33.7503574492422	37.0	34.0	38.0	21.5	38.0
34-35	33.80833571632828	37.0	34.0	38.0	22.0	38.0
36-37	31.086931655704888	36.5	27.5	38.0	11.0	38.0
38-39	28.799542464969974	34.0	22.0	37.5	11.0	38.0
40-41	30.371818701744353	34.5	25.5	37.0	11.0	38.0
42-43	31.969831283957674	36.5	30.5	37.5	16.0	38.0
44-45	33.90148698884758	37.0	34.0	38.0	23.5	38.0
46-47	32.19302259079211	36.5	29.0	38.0	17.0	38.0
48-49	31.33964826994567	35.5	27.5	38.0	16.0	38.0
50-51	28.697669430940806	34.0	22.0	37.0	11.0	38.0
52-53	32.676079496711466	36.5	32.5	38.0	21.0	38.0
54-55	31.644552473548757	36.5	28.5	38.0	16.0	38.0
56-57	32.348655990849295	36.5	30.5	38.0	17.0	38.0
58-59	33.64505290248785	37.0	34.0	38.0	22.5	38.0
60-61	34.2078209894195	37.0	34.0	38.0	23.5	38.0
62-63	34.05368887617958	37.0	34.0	38.0	23.0	38.0
64-65	33.63318558764655	37.5	34.5	38.0	22.0	38.0
66-67	30.96604232199028	36.5	26.0	38.0	11.0	38.0
68-69	31.131469831283958	36.0	26.0	38.0	11.0	38.0
70-71	30.471404060623392	35.0	25.5	38.0	11.0	38.0
72-73	29.22562196168144	34.0	23.5	37.0	11.0	38.0
74-75	31.823491564197884	36.0	29.5	37.5	16.5	38.0
76	32.150986559908496	36.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	14.0
15	20.0
16	40.0
17	47.0
18	86.0
19	93.0
20	87.0
21	85.0
22	89.0
23	142.0
24	132.0
25	165.0
26	172.0
27	214.0
28	244.0
29	289.0
30	381.0
31	472.0
32	627.0
33	779.0
34	853.0
35	966.0
36	750.0
37	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.855876465541893	22.261938804689734	13.511581355447527	32.370603374320844
2	17.200457535030026	16.34257935373177	45.12439233628825	21.332570774949957
3	16.27108950529025	19.988561624249357	39.6053760366028	24.134972833857592
4	12.567915356019446	19.144981412639407	35.60194452387761	32.68515870746354
5	10.966542750929369	32.1990277380612	41.07806691449814	15.756362596511295
6	23.677437803831854	38.80468973405776	17.357735201601372	20.16013726050901
7	23.634543894766942	27.823849013440093	23.405776379754077	25.135830712038892
8	18.773234200743495	36.302545038604514	18.944809837003145	25.979410923648842
9	19.745496139548184	15.19874177866743	22.891049470975123	42.16471261080926
10-11	21.34686874463826	25.91507006005147	29.303688876179585	23.434372319130684
12-13	20.574778381469834	20.932227623677438	32.31341149556763	26.179582499285104
14-15	21.025164426651415	25.450386045181585	24.270803545896484	29.253645982270516
16-17	23.477266228195596	25.87932513583071	25.90792107520732	24.73548756076637
18-19	18.944809837003145	25.67915356019445	29.22505004289391	26.150986559908496
20-21	22.69087789533886	25.51472690877895	28.167000285959393	23.627394909922792
22-23	23.284243637403492	24.06348298541607	28.12410637689448	24.528167000285958
24-25	19.888475836431226	27.68086931655705	25.664855590506146	26.765799256505574
26-27	21.53274235058622	26.136688590220185	27.559336574206462	24.77123248498713
28-29	20.224478124106376	27.023162710895054	27.659422362024593	25.092936802973973
30-31	20.03145553331427	27.99542464969974	27.023162710895054	24.949957106090935
32-33	19.90277380611953	27.38776093794681	27.344867028881897	25.36459822705176
34-35	19.881326851587076	27.273377180440377	28.109808407206177	24.73548756076637
36-37	22.869602516442665	25.135830712038892	30.240205890763512	21.754360880754934
38-39	21.625679153560196	24.25650557620818	33.335716328281386	20.782098941950245
40-41	19.052044609665426	27.430654847011727	25.693451529882754	27.823849013440093
42-43	18.251358307120388	28.881898770374608	26.150986559908496	26.715756362596508
44-45	19.95996568487275	27.04460966542751	27.75235916499857	25.243065484701173
46-47	21.91878753217043	26.458392908207035	28.174149270803543	23.448670288818988
48-49	22.42636545610523	26.622819559622535	27.31627108950529	23.634543894766942
50-51	25.614812696597085	24.05633400057192	28.274235058621677	22.054618244209323
52-53	19.809837003145553	27.20903631684301	28.109808407206177	24.87131827280526
54-55	21.404060623391477	26.79439519588219	27.866742922505004	23.934801258221334
56-57	20.83929082070346	27.61652845295968	26.75150128681727	24.79267943951959
58-59	19.73834715470403	28.410065770660566	27.38776093794681	24.463826136688592
60-61	19.245067200457537	27.47354875607664	27.902487846725766	25.378896196740065
62-63	17.522161853016872	28.617386331140978	28.345724907063197	25.51472690877895
64-65	19.52387760937947	29.71832999714041	27.158993422933943	23.598798970546184
66-67	16.657134686874464	29.496711466971693	25.643408635973692	28.20274521018015
68-69	17.215986272967758	28.426395939086298	26.660470436834206	27.697147351111745
70-71	17.82241921647126	29.303688876179585	25.30025736345439	27.573634543894766
72-73	17.21475550471833	28.417214755504716	23.949099227909638	30.418930511867316
74-75	18.880468973405776	28.267086073777527	27.745210180154416	25.107234772662284
76	19.445238776093795	27.55218758936231	27.75235916499857	25.250214469545323
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	1.5
19	2.5
20	5.5
21	8.0
22	8.5
23	11.0
24	17.0
25	21.0
26	30.5
27	43.0
28	50.0
29	54.0
30	71.0
31	95.5
32	124.0
33	145.0
34	158.0
35	207.5
36	256.5
37	269.0
38	287.0
39	328.0
40	402.0
41	441.0
42	429.0
43	444.5
44	453.5
45	457.0
46	467.0
47	445.5
48	406.5
49	367.5
50	346.0
51	320.5
52	266.0
53	228.5
54	220.0
55	184.5
56	137.0
57	115.0
58	105.0
59	98.0
60	82.5
61	65.0
62	56.0
63	50.5
64	41.0
65	41.5
66	41.5
67	37.0
68	33.0
69	28.0
70	33.0
71	39.0
72	29.0
73	17.5
74	14.0
75	12.0
76	15.0
77	13.5
78	7.5
79	6.0
80	5.5
81	4.0
82	2.5
83	2.0
84	1.0
85	1.0
86	1.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.007148984844152131
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	6994.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.9991421218187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.06123627960717	98.06977409207892
2	0.8665511265164645	1.7157563625965113
3	0.07221259387637204	0.21446954532456391
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.014297969688304261	0.0
12	0.0	0.0	0.0	0.014297969688304261	0.0
13	0.0	0.0	0.0	0.014297969688304261	0.0
14	0.0	0.0	0.0	0.014297969688304261	0.0
15	0.0	0.0	0.0	0.014297969688304261	0.0
16	0.0	0.0	0.0	0.014297969688304261	0.0
17	0.0	0.0	0.0	0.014297969688304261	0.0
18	0.0	0.0	0.0	0.014297969688304261	0.0
19	0.0	0.0	0.0	0.028595939376608523	0.0
20	0.0	0.0	0.0	0.042893909064912784	0.0
21	0.0	0.0	0.0	0.042893909064912784	0.0
22	0.0	0.0	0.0	0.042893909064912784	0.0
23	0.0	0.0	0.0	0.057191878753217046	0.0
24	0.0	0.0	0.0	0.057191878753217046	0.0
25	0.0	0.0	0.0	0.057191878753217046	0.0
26	0.0	0.0	0.0	0.057191878753217046	0.0
27	0.0	0.0	0.0	0.057191878753217046	0.0
28	0.0	0.0	0.0	0.057191878753217046	0.0
29	0.0	0.0	0.0	0.057191878753217046	0.0
30	0.0	0.0	0.0	0.057191878753217046	0.0
31	0.0	0.0	0.0	0.057191878753217046	0.0
32	0.0	0.0	0.0	0.057191878753217046	0.0
33	0.0	0.0	0.0	0.057191878753217046	0.0
34	0.0	0.0	0.0	0.057191878753217046	0.0
35	0.0	0.0	0.0	0.0714898484415213	0.0
36	0.0	0.0	0.0	0.0714898484415213	0.0
37	0.0	0.0	0.0	0.11438375750643409	0.0
38	0.0	0.0	0.0	0.11438375750643409	0.0
39	0.0	0.0	0.0	0.11438375750643409	0.0
40	0.0	0.0	0.0	0.11438375750643409	0.0
41	0.0	0.0	0.0	0.11438375750643409	0.0
42	0.0	0.0	0.0	0.12868172719473836	0.0
43	0.0	0.0	0.0	0.17157563625965114	0.0
44	0.0	0.0	0.0	0.17157563625965114	0.0
45	0.0	0.0	0.0	0.18587360594795538	0.0
46	0.0	0.0	0.0	0.22876751501286818	0.0
47	0.0	0.0	0.0	0.24306548470117242	0.0
48	0.0	0.0	0.0	0.2573634543894767	0.0
49	0.0	0.0	0.0	0.27166142407778093	0.0
50	0.0	0.0	0.0	0.2859593937660852	0.0
51	0.0	0.0	0.0	0.3431512725193023	0.0
52	0.0	0.0	0.0	0.37174721189591076	0.0
53	0.0	0.0	0.0	0.41464112096082356	0.0
54	0.0	0.0	0.0	0.4432370603374321	0.0
55	0.0	0.0	0.0	0.48613096940234485	0.0
56	0.0	0.0	0.0	0.5290248784672577	0.0
57	0.0	0.0	0.0	0.5576208178438662	0.0
58	0.0	0.0	0.0	0.6005147269087789	0.0
59	0.0	0.0	0.0	0.7006005147269088	0.0
60	0.0	0.0	0.0	0.7434944237918215	0.0
61	0.0	0.0	0.0	0.7434944237918215	0.0
62	0.0	0.0	0.0	0.7577923934801258	0.0
63	0.0	0.0	0.0	0.77209036316843	0.0
64	0.0	0.0	0.0	0.7863883328567344	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE