FastQCFastQC Report
Wed 25 May 2016
SRR1779704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779704_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6994
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT460.657706605661996No Hit
CATATAACCTCCCCCAAAATTCAGAATAATAACACACCCGACCACACCGC100.14297969688304263No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT100.14297969688304263RNA PCR Primer, Index 47 (95% over 21bp)
GATTAGTAGTATGGGAGTGGGAGGGGAAAATAATGTGTTAGTTGGGGGGT100.14297969688304263No Hit
TAATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAA90.12868172719473833No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA90.12868172719473833No Hit
GGATATAGGGTCGAAGCCGCACTCGTAAGGGGTGGATTTTTCTATGTAGC80.11438375750643409No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG80.11438375750643409No Hit
GTAGGGGAAGGGAGCCTACTAGGGTGTAGAATAGGAAGTATGTGCCTGCG70.10008578781812982No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG70.10008578781812982No Hit
ATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACCACCTACCTC70.10008578781812982No Hit
GTAGAAGAGCGATGGTGAGAGCTAAGGTCGGGGCGGTGATGTAGAGGGTG70.10008578781812982No Hit
GGTTGAGGTTGACCAGGGGGTTGGGTATGGGGAGGGGGGTTCATAGTAGA70.10008578781812982No Hit
ATATTATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGC70.10008578781812982No Hit
GTATTAGGAGGGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAG70.10008578781812982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT307.5403914E-658.3333365
TTATACA252.228978E-455.9999962
CTTATAC252.228978E-455.9999961
ACACATC351.8792802E-550.06
CACATCT305.4832024E-446.6666687
TATACAC305.4832024E-446.6666683
GAGCCCA305.4832024E-446.66666816
CGAGCCC305.4832024E-446.66666815
ATACACA305.4832024E-446.6666684
AGCCCAC350.001171634140.017
GCCCACG400.00225827435.018
Produced by FastQC (version 0.11.5)