##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779703_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7432
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.935279870828847	34.0	32.0	34.0	21.0	34.0
2	29.08840150699677	34.0	24.0	34.0	12.0	34.0
3	28.175188374596342	34.0	23.0	34.0	11.0	34.0
4	31.005516684607105	34.0	32.0	34.0	23.0	34.0
5	30.36302475780409	34.0	32.0	34.0	21.0	34.0
6	33.20869214208827	37.0	34.0	38.0	21.0	38.0
7	34.20250269106566	38.0	34.0	38.0	24.0	38.0
8	33.334364908503765	38.0	34.0	38.0	21.0	38.0
9	34.85898815931109	38.0	36.0	38.0	26.0	38.0
10-11	34.65345801937567	38.0	36.0	38.0	25.0	38.0
12-13	33.3807857911733	37.5	32.0	38.0	19.0	38.0
14-15	33.69671689989236	37.5	34.0	38.0	22.0	38.0
16-17	33.27650699677072	37.5	31.5	38.0	19.0	38.0
18-19	33.9330597416577	38.0	34.0	38.0	23.0	38.0
20-21	32.72571313240043	37.0	30.5	38.0	17.5	38.0
22-23	32.66227125941873	37.5	31.0	38.0	18.5	38.0
24-25	34.27321044133477	37.5	35.0	38.0	24.5	38.0
26-27	32.41220398277718	37.0	30.5	38.0	16.0	38.0
28-29	34.83248116254037	38.0	36.0	38.0	26.0	38.0
30-31	35.264397201291715	38.0	37.0	38.0	27.0	38.0
32-33	35.25679494079655	38.0	37.0	38.0	27.0	38.0
34-35	35.40527448869753	38.0	37.0	38.0	29.5	38.0
36-37	33.060347147470395	37.5	30.5	38.0	17.5	38.0
38-39	31.011167922497307	36.5	26.5	38.0	11.0	38.0
40-41	32.58793057050592	37.0	30.5	38.0	16.0	38.0
42-43	33.7543057050592	37.5	34.0	38.0	18.5	38.0
44-45	35.39504843918192	38.0	37.0	38.0	29.0	38.0
46-47	33.916846071044134	37.5	34.0	38.0	21.0	38.0
48-49	33.36127556512379	37.5	33.0	38.0	18.5	38.0
50-51	30.79709364908504	36.5	25.5	38.0	11.0	38.0
52-53	34.61067007534984	37.5	35.0	38.0	26.0	38.0
54-55	33.65756189451022	37.5	33.5	38.0	18.5	38.0
56-57	34.09095801937568	38.0	35.5	38.0	19.0	38.0
58-59	35.248856297093646	38.0	36.5	38.0	29.0	38.0
60-61	35.53848223896663	38.0	37.0	38.0	30.5	38.0
62-63	35.45822120559742	38.0	37.0	38.0	29.0	38.0
64-65	35.23250807319699	38.0	37.0	38.0	27.5	38.0
66-67	33.12022335844995	37.5	34.0	38.0	11.0	38.0
68-69	33.15769644779333	37.0	33.0	38.0	16.0	38.0
70-71	32.470129171151775	37.0	30.5	38.0	15.0	38.0
72-73	31.42471743810549	36.5	27.0	38.0	11.0	38.0
74-75	33.86773412271259	37.5	34.5	38.0	24.0	38.0
76	34.51829924650161	37.0	35.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	4.0
14	19.0
15	30.0
16	49.0
17	59.0
18	89.0
19	76.0
20	68.0
21	54.0
22	55.0
23	58.0
24	62.0
25	71.0
26	60.0
27	97.0
28	116.0
29	144.0
30	172.0
31	256.0
32	371.0
33	587.0
34	881.0
35	1289.0
36	1753.0
37	1009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.33799784714747	21.407427341227127	12.970936490850377	31.283638320775026
2	17.868675995694293	18.393433799784713	40.16415500538213	23.57373519913886
3	18.729817007534987	20.546286329386437	33.90742734122713	26.816469321851454
4	12.244348762109796	19.294940796555434	36.28902045209903	32.17168998923574
5	11.504305705059203	33.10010764262648	39.2491926803014	16.146393972012916
6	24.192680301399356	36.692680301399356	17.855220667384284	21.259418729817007
7	23.963939720129172	29.60172228202368	23.2508073196986	23.183530678148546
8	20.021528525296016	34.41872981700754	19.792787944025832	25.766953713670617
9	20.882669537136707	15.446716899892357	21.555435952637243	42.115177610333696
10-11	22.22147470398278	25.329655543595265	28.155274488697522	24.293595263724434
12-13	21.965823466092573	22.01291711517761	28.3772874058127	27.643972012917118
14-15	21.319967707212058	23.57373519913886	24.78471474703983	30.321582346609254
16-17	21.905274488697522	25.52475780409042	27.025026910656617	25.544940796555437
18-19	20.33772874058127	25.666038751345532	27.132669537136707	26.86356297093649
20-21	21.171959095801938	25.222012917115176	27.650699677072122	25.955328310010767
22-23	21.67653390742734	24.488697524219592	28.094725511302478	25.740043057050592
24-25	20.768299246501616	26.305166846071042	25.376749192680304	27.549784714747037
26-27	20.667384284176535	25.32292787944026	28.175457481162542	25.834230355220665
28-29	20.70102260495156	27.85252960172228	26.668460710441334	24.777987082884824
30-31	20.573196986006458	27.395048439181917	26.271528525296016	25.760226049515605
32-33	20.505920344456406	27.21340150699677	26.702099031216363	25.578579117330463
34-35	21.34687836383208	25.96205597416577	26.73573735199139	25.955328310010767
36-37	21.629440258342303	24.44833153928956	29.440258342303554	24.481969860064588
38-39	21.60252960172228	23.970667384284177	31.532561894510224	22.894241119483315
40-41	21.11141011840689	24.730893433799785	25.874596340150703	28.28310010764263
42-43	19.893702906350917	25.444025834230356	26.856835306781484	27.805435952637243
44-45	19.429494079655544	26.264800861141012	27.643972012917118	26.66173304628633
46-47	21.272874058127016	25.053821313240043	28.619483315392895	25.053821313240043
48-49	21.64962325080732	26.412809472551128	27.3748654467169	24.56270182992465
50-51	22.477125941872984	23.03552206673843	30.079386437029065	24.407965554359524
52-53	19.853336921420883	26.68864370290635	27.368137782561895	26.08988159311087
54-55	21.703444564047363	26.251345532831	26.607911733046286	25.43729817007535
56-57	20.680839612486544	26.1235199138859	27.34795479009688	25.84768568353068
58-59	19.826426264800862	27.206673842841766	26.89047362755651	26.076426264800862
60-61	19.954251883745965	26.607911733046286	26.971205597416574	26.466630785791175
62-63	18.884553283100107	26.73573735199139	28.10145317545748	26.27825618945102
64-65	19.72551130247578	27.590150699677075	27.226856835306783	25.45748116254037
66-67	18.736544671689987	25.430570505920347	24.643433799784713	31.189451022604953
68-69	18.253380878692056	26.616430061225866	25.681221826010898	29.448967234071183
70-71	19.254574811625403	25.592034445640472	25.349838536060282	29.803552206673846
72-73	18.22524219590958	25.18164693218515	24.582884822389666	32.01022604951561
74-75	19.180570505920343	27.233584499461788	26.042787944025836	27.543057050592033
76	20.855758880516685	26.04951560818084	26.170613562970935	26.924111948331543
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	3.0
21	6.0
22	6.0
23	8.0
24	15.5
25	21.0
26	20.5
27	25.5
28	45.0
29	59.0
30	61.0
31	82.0
32	110.5
33	120.0
34	140.5
35	188.5
36	244.5
37	273.0
38	293.5
39	335.0
40	381.0
41	431.0
42	456.0
43	455.0
44	456.0
45	444.0
46	430.0
47	449.5
48	464.5
49	405.0
50	350.0
51	343.0
52	303.0
53	237.5
54	205.0
55	197.5
56	173.5
57	141.0
58	125.0
59	116.0
60	90.5
61	70.5
62	67.0
63	66.0
64	59.5
65	59.0
66	64.0
67	64.0
68	62.0
69	62.5
70	64.0
71	63.0
72	53.0
73	48.0
74	50.0
75	47.0
76	38.5
77	24.0
78	14.0
79	10.0
80	11.0
81	8.0
82	3.5
83	3.0
84	3.5
85	2.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.006727664155005381
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	7432.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.7529601722282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.0041293874742	95.80193756727664
2	1.7481073640743288	3.417653390742734
3	0.20646937370956642	0.6054897739504844
4	0.02752924982794219	0.1076426264800861
5	0.013764624913971095	0.06727664155005382
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.013455328310010764	0.0
22	0.0	0.0	0.0	0.06727664155005382	0.0
23	0.0	0.0	0.0	0.06727664155005382	0.0
24	0.0	0.0	0.0	0.08073196986006459	0.0
25	0.0	0.0	0.0	0.08073196986006459	0.0
26	0.0	0.0	0.0	0.08073196986006459	0.0
27	0.0	0.0	0.0	0.08073196986006459	0.0
28	0.0	0.0	0.0	0.08073196986006459	0.0
29	0.0	0.0	0.0	0.08073196986006459	0.0
30	0.0	0.0	0.0	0.08073196986006459	0.0
31	0.0	0.0	0.0	0.08073196986006459	0.0
32	0.0	0.0	0.0	0.08073196986006459	0.0
33	0.0	0.0	0.0	0.09418729817007535	0.0
34	0.0	0.0	0.0	0.12109795479009688	0.0
35	0.0	0.0	0.0	0.13455328310010764	0.0
36	0.0	0.0	0.0	0.1480086114101184	0.0
37	0.0	0.0	0.0	0.17491926803013993	0.0
38	0.0	0.0	0.0	0.21528525296017223	0.0
39	0.0	0.0	0.0	0.22874058127018299	0.0
40	0.0	0.0	0.0	0.2556512378902045	0.0
41	0.0	0.0	0.0	0.2691065662002153	0.0
42	0.0	0.0	0.0	0.34983853606027987	0.0
43	0.0	0.0	0.0	0.3767491926803014	0.0
44	0.0	0.0	0.0	0.48439181916038754	0.0
45	0.0	0.0	0.0	0.5247578040904198	0.0
46	0.0	0.0	0.0	0.6054897739504844	0.0
47	0.0	0.0	0.0	0.686221743810549	0.0
48	0.0	0.0	0.0	0.7265877287405813	0.0
49	0.0	0.0	0.0	0.8073196986006459	0.0
50	0.0	0.0	0.0	0.8476856835306782	0.0
51	0.0	0.0	0.0	0.9553283100107642	0.0
52	0.0	0.0	0.0	1.036060279870829	0.0
53	0.0	0.0	0.0	1.143702906350915	0.0
54	0.0	0.0	0.0	1.1840688912809472	0.0
55	0.0	0.0	0.0	1.2244348762109796	0.0
56	0.0	0.0	0.0	1.2917115177610334	0.0
57	0.0	0.0	0.0	1.372443487621098	0.0
58	0.0	0.0	0.0	1.3993541442411195	0.0
59	0.0	0.0	0.0	1.547362755651238	0.0
60	0.0	0.0	0.0	1.5877287405812701	0.0
61	0.0	0.0	0.0	1.6280947255113025	0.0
62	0.0	0.0	0.0	1.6819160387513454	0.0
63	0.0	0.0	0.0	1.76264800861141	0.0
64	0.0	0.0	0.0	1.8299246501614639	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAATCA	15	0.0021383327	70.00001	4
CATAATC	20	0.0066835545	52.5	3
>>END_MODULE