FastQCFastQC Report
Wed 25 May 2016
SRR1779703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779703_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7432
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1081.4531754574811624No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT360.48439181916038754Illumina PCR Primer Index 9 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC210.28256189451022606No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC210.28256189451022606No Hit
GTGTATGGTTATCATTTGTTTTGAGGTTAGTTTGATTAGTCATTGTTGGG120.16146393972012918No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT110.1480086114101184No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC110.1480086114101184No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT90.12109795479009688No Hit
GTGTAGGAAGAGGCAGATAAAGAATATTGAGGCGCCATTGGCGTGAAGGT90.12109795479009688No Hit
ATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCA80.1076426264800861No Hit
CCCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACAT80.1076426264800861No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC350.001176669340.042
TGCCGTC350.001176669340.045
TATGCCG350.001176669340.043
CCGTCTT350.001176669340.047
ATGCCGT350.001176669340.044
GTCTTCT350.001176669340.049
CGTATGC350.001176669340.041
GCTTGAA350.001176669340.056
CTGCTTG350.001176669340.054
TCGTATG350.001176669340.040
TTGAAAA350.001176669340.058
GCCGTCT350.001176669340.046
CTTGAAA350.001176669340.057
TCTTCTG350.001176669340.050
CGTCTTC350.001176669340.048
CTCGTAT350.001176669340.039
CTTCTGC350.001176669340.051
CGTTAAC400.002267920635.028
ACGTTAA400.002267920635.027
TCTGCTT400.002267920635.053