##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779702_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12690
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.282505910165483	34.0	32.0	34.0	23.0	34.0
2	29.29684791174153	34.0	27.0	34.0	12.0	34.0
3	28.326319936958235	34.0	23.0	34.0	11.0	34.0
4	31.139243498817965	34.0	32.0	34.0	23.0	34.0
5	30.591095350669818	34.0	32.0	34.0	21.0	34.0
6	33.35027580772262	37.0	34.0	38.0	21.0	38.0
7	34.45949566587864	38.0	34.0	38.0	26.0	38.0
8	33.63940110323089	38.0	34.0	38.0	21.0	38.0
9	35.09795114263199	38.0	36.0	38.0	27.0	38.0
10-11	34.943538219070135	38.0	36.0	38.0	26.5	38.0
12-13	33.641686367218284	37.5	32.0	38.0	21.0	38.0
14-15	33.846926713947994	37.5	34.0	38.0	22.5	38.0
16-17	33.4414499605989	37.5	32.0	38.0	19.0	38.0
18-19	34.05488573680063	37.5	34.0	38.0	23.5	38.0
20-21	32.9644208037825	37.0	30.5	38.0	18.5	38.0
22-23	32.955594956658786	37.5	31.0	38.0	19.0	38.0
24-25	34.462056737588654	37.5	35.0	38.0	25.0	38.0
26-27	32.70307328605201	37.0	30.5	38.0	16.5	38.0
28-29	35.153585500394016	38.0	36.5	38.0	27.0	38.0
30-31	35.516666666666666	38.0	37.0	38.0	31.0	38.0
32-33	35.62490149724192	38.0	37.0	38.0	31.0	38.0
34-35	35.67352245862884	38.0	37.0	38.0	31.0	38.0
36-37	33.40681639085894	38.0	33.0	38.0	18.5	38.0
38-39	31.235657998423957	36.5	26.5	38.0	11.0	38.0
40-41	32.883884948778565	37.0	32.5	38.0	17.0	38.0
42-43	34.02612293144208	37.5	35.0	38.0	19.0	38.0
44-45	35.64215918045706	38.0	37.0	38.0	31.5	38.0
46-47	34.08416075650118	38.0	34.0	38.0	21.5	38.0
48-49	33.578526398739164	37.5	33.5	38.0	18.5	38.0
50-51	31.129472025216707	36.5	26.5	38.0	11.0	38.0
52-53	34.799290780141845	37.5	35.5	38.0	28.5	38.0
54-55	33.873246650906225	37.5	34.0	38.0	19.0	38.0
56-57	34.35252167060678	38.0	35.5	38.0	21.0	38.0
58-59	35.4246256895193	38.0	37.0	38.0	31.0	38.0
60-61	35.823443656422384	38.0	37.0	38.0	32.0	38.0
62-63	35.73999211977935	38.0	37.0	38.0	32.5	38.0
64-65	35.456422379826634	38.0	37.0	38.0	29.5	38.0
66-67	33.139401103230895	37.5	33.5	38.0	16.0	38.0
68-69	33.32427107959023	37.0	33.5	38.0	16.5	38.0
70-71	32.71532702915682	37.0	32.5	38.0	16.0	38.0
72-73	31.34523246650906	36.5	27.0	38.0	11.0	38.0
74-75	34.080102442868395	37.5	35.0	38.0	24.0	38.0
76	34.67911741528763	37.0	36.0	38.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	7.0
14	19.0
15	58.0
16	66.0
17	86.0
18	118.0
19	111.0
20	114.0
21	91.0
22	79.0
23	73.0
24	80.0
25	83.0
26	104.0
27	151.0
28	173.0
29	239.0
30	299.0
31	450.0
32	660.0
33	986.0
34	1548.0
35	2490.0
36	2977.0
37	1628.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.30260047281324	20.41765169424744	10.37037037037037	34.909377462568955
2	19.09377462568952	19.605988967691097	35.68951930654058	25.6107171000788
3	17.659574468085108	18.51063829787234	32.88416075650118	30.94562647754137
4	12.2301024428684	17.383766745468872	33.766745468873125	36.6193853427896
5	12.261623325453112	30.346729708431834	38.2033096926714	19.188337273443658
6	25.429472025216704	35.37431048069346	17.013396375098502	22.18282111899133
7	26.02836879432624	29.676910953506695	20.62253743104807	23.67218282111899
8	21.56028368794326	32.671394799054376	18.786446020488572	26.98187549251379
9	20.921985815602838	15.760441292356187	19.55082742316785	43.76674546887313
10-11	21.72970843183609	24.74389282899921	27.10795902285264	26.418439716312058
12-13	21.95035460992908	21.56816390858944	28.43577620173365	28.045705279747835
14-15	19.901497241922776	23.774625689519308	24.08589440504334	32.23798266351458
16-17	21.185973207249802	25.78802206461781	26.300236406619387	26.725768321513005
18-19	19.475965327029158	26.970055161544522	26.351457840819542	27.202521670606778
20-21	21.16233254531127	25.508274231678485	27.419227738376673	25.91016548463357
22-23	20.721040189125294	25.65011820330969	27.750197005516153	25.878644602048855
24-25	19.653270291568166	27.490149724192275	25.516154452324663	27.340425531914892
26-27	19.972419227738378	25.65799842395587	28.400315208825848	25.969267139479907
28-29	19.59022852639874	27.383766745468876	26.39873916469661	26.627265563435778
30-31	19.87391646966115	27.190701339637506	26.773049645390074	26.16233254531127
32-33	19.34200157604413	27.253743104806933	26.528762805358554	26.875492513790388
34-35	20.0354609929078	27.28132387706856	26.091410559495664	26.591804570527977
36-37	20.70527974783294	25.799842395587074	28.735224586288417	24.75965327029157
38-39	20.61859732072498	25.200945626477537	31.22931442080378	22.951142631993697
40-41	20.271867612293143	24.692671394799053	26.063829787234045	28.97163120567376
42-43	19.20409771473601	25.744680851063826	26.04018912529551	29.01103230890465
44-45	19.148936170212767	27.013396375098502	27.620173364854217	26.217494089834513
46-47	19.90937746256895	26.24507486209614	27.328605200945628	26.516942474389282
48-49	19.95665878644602	26.836091410559497	27.43104806934594	25.77620173364854
50-51	20.760441292356184	25.236406619385342	29.830575256107174	24.1725768321513
52-53	19.25925925925926	26.717888100866826	27.364066193853425	26.65878644602049
54-55	20.08668242710796	26.93065405831363	27.356185973207246	25.626477541371155
56-57	19.822695035460995	26.970055161544522	27.249802994483847	25.957446808510635
58-59	19.739952718676122	27.442868400315206	26.773049645390074	26.044129235618595
60-61	18.88888888888889	26.85579196217494	27.143420015760437	27.111899133175726
62-63	18.29787234042553	27.324665090622535	27.60441292356186	26.773049645390074
64-65	18.786446020488572	28.179669030732864	27.001576044129234	26.03230890464933
66-67	18.573680063041763	25.413711583924346	25.307328605200947	30.705279747832943
68-69	18.10766078184111	26.525063051702396	25.457124842370742	29.91015132408575
70-71	18.68794326241135	24.83057525610717	25.724980299448386	30.756501182033098
72-73	17.52955082742317	24.822695035460992	24.739952718676122	32.90780141843971
74-75	18.821907013396373	27.395587076438137	26.457840819542948	27.324665090622535
76	19.148936170212767	26.721828211189912	27.39952718676123	26.72970843183609
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	1.0
17	1.0
18	1.0
19	2.5
20	4.0
21	4.0
22	5.5
23	9.5
24	18.0
25	24.0
26	30.5
27	50.0
28	84.0
29	105.0
30	108.0
31	136.0
32	188.0
33	215.0
34	252.0
35	328.0
36	384.5
37	402.0
38	459.0
39	580.5
40	663.0
41	694.5
42	708.0
43	729.0
44	774.0
45	802.0
46	806.0
47	799.5
48	751.5
49	671.0
50	632.0
51	609.5
52	529.0
53	437.5
54	404.0
55	359.5
56	286.0
57	237.0
58	217.0
59	202.5
60	166.5
61	130.0
62	115.0
63	110.5
64	108.5
65	117.5
66	121.0
67	118.0
68	117.5
69	107.5
70	115.0
71	132.0
72	113.0
73	84.5
74	58.0
75	41.0
76	37.5
77	31.0
78	25.0
79	22.0
80	22.0
81	14.5
82	6.0
83	5.0
84	5.5
85	4.0
86	2.0
87	2.0
88	1.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.015760441292356188
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	12690.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.67454688731284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.34268014346267	94.10559495665879
2	2.135637430714053	4.1292356185973205
3	0.38311053146397134	1.1111111111111112
4	0.06521030322791001	0.252167060677699
5	0.05705901532442126	0.27580772261623326
6	0.0	0.0
7	0.008151287903488751	0.055161544523246654
8	0.0	0.0
9	0.008151287903488751	0.07092198581560284
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.007880220646178092	0.0
21	0.0	0.0	0.0	0.007880220646178092	0.0
22	0.0	0.0	0.0	0.03152088258471237	0.0
23	0.0	0.0	0.0	0.03152088258471237	0.0
24	0.0	0.0	0.0	0.055161544523246654	0.0
25	0.0	0.0	0.0	0.055161544523246654	0.0
26	0.0	0.0	0.0	0.055161544523246654	0.0
27	0.0	0.0	0.0	0.06304176516942474	0.0
28	0.0	0.0	0.0	0.06304176516942474	0.0
29	0.0	0.0	0.0	0.06304176516942474	0.0
30	0.0	0.0	0.0	0.06304176516942474	0.0
31	0.0	0.0	0.0	0.06304176516942474	0.0
32	0.0	0.0	0.0	0.06304176516942474	0.0
33	0.0	0.0	0.0	0.07092198581560284	0.0
34	0.0	0.0	0.0	0.07880220646178093	0.0
35	0.0	0.0	0.0	0.08668242710795902	0.0
36	0.0	0.0	0.0	0.09456264775413711	0.0
37	0.0	0.0	0.0	0.1182033096926714	0.0
38	0.0	0.0	0.0	0.13396375098502758	0.0
39	0.0	0.0	0.0	0.16548463356973994	0.0
40	0.0	0.0	0.0	0.22064617809298662	0.0
41	0.0	0.0	0.0	0.26792750197005516	0.0
42	0.0	0.0	0.0	0.3624901497241923	0.0
43	0.0	0.0	0.0	0.44129235618597323	0.0
44	0.0	0.0	0.0	0.5358550039401103	0.0
45	0.0	0.0	0.0	0.6304176516942475	0.0
46	0.0	0.0	0.0	0.7092198581560284	0.0
47	0.0	0.0	0.0	0.8195429472025216	0.0
48	0.0	0.0	0.0	0.8747044917257684	0.0
49	0.0	0.0	0.0	0.929866036249015	0.0
50	0.0	0.0	0.0	1.0480693459416863	0.0
51	0.0	0.0	0.0	1.1426319936958236	0.0
52	0.0	0.0	0.0	1.2450748620961387	0.0
53	0.0	0.0	0.0	1.426319936958235	0.0
54	0.0	0.0	0.0	1.615445232466509	0.0
55	0.0	0.0	0.0	1.7257683215130024	0.0
56	0.0	0.0	0.0	1.867612293144208	0.0
57	0.0	0.0	0.0	1.9858156028368794	0.0
58	0.0	0.0	0.0	2.033096926713948	0.0
59	0.0	0.0	0.0	2.19070133963751	0.0
60	0.0	0.0	0.0	2.316784869976359	0.0
61	0.0	0.0	0.0	2.39558707643814	0.0
62	0.0	0.0	0.0	2.5059101654846336	0.0
63	0.0	0.0	0.0	2.624113475177305	0.0
64	0.0	0.0	0.0	2.7501970055161546	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTCAC	15	0.0021776587	69.97233	8
TATATTT	20	0.0068055824	52.479248	45
ATATTTT	20	0.0068055824	52.479248	46
>>END_MODULE