FastQCFastQC Report
Wed 25 May 2016
SRR1779702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779702_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12690
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT790.6225374310480694No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT360.28368794326241137RNA PCR Primer, Index 42 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC240.18912529550827423No Hit
GTGTAGGAAGAGGCAGATAAAGAATATTGAGGCGCCATTGGCGTGAAGGT220.17336485421591805No Hit
GGGTGGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTG190.14972419227738376No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA160.1260835303388495No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT160.1260835303388495No Hit
GGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGGGATGGGTTCGA140.11032308904649331No Hit
GTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAATAAAA130.1024428684003152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGAG150.002174246870.0000137
TAAGTAG150.002174246870.0000117
GAAAAAA657.5303926E-426.92307757
AAAAAAA2902.7927806E-410.86206962
Produced by FastQC (version 0.11.5)