##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779702_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12690
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03215130023641	34.0	34.0	34.0	31.0	34.0
2	33.259574468085106	34.0	34.0	34.0	32.0	34.0
3	33.29054373522459	34.0	34.0	34.0	33.0	34.0
4	33.39960598896769	34.0	34.0	34.0	34.0	34.0
5	33.33624901497242	34.0	34.0	34.0	34.0	34.0
6	36.582111899133174	38.0	37.0	38.0	34.0	38.0
7	36.62939322301025	38.0	37.0	38.0	34.0	38.0
8	36.15437352245863	38.0	37.0	38.0	34.0	38.0
9	36.08313632781718	38.0	36.0	38.0	32.0	38.0
10-11	36.18234830575256	38.0	37.0	38.0	34.0	38.0
12-13	36.21674546887313	38.0	37.0	38.0	34.0	38.0
14-15	36.21446020488574	38.0	37.0	38.0	34.0	38.0
16-17	36.17635933806147	38.0	37.0	38.0	33.5	38.0
18-19	36.20693459416864	38.0	37.0	38.0	34.0	38.0
20-21	36.16130811662727	38.0	37.0	38.0	33.5	38.0
22-23	35.957013396375096	38.0	36.0	38.0	32.0	38.0
24-25	36.013120567375886	38.0	36.0	38.0	32.0	38.0
26-27	35.91201733648542	38.0	36.0	38.0	31.5	38.0
28-29	35.77352245862884	38.0	36.0	38.0	31.5	38.0
30-31	35.748305752561066	38.0	36.0	38.0	31.0	38.0
32-33	35.730457052797476	38.0	36.0	38.0	31.0	38.0
34-35	35.72163120567376	38.0	36.0	38.0	31.0	38.0
36-37	35.80409771473602	38.0	36.0	38.0	31.5	38.0
38-39	35.79369582348306	38.0	36.0	38.0	31.5	38.0
40-41	35.959771473601265	38.0	36.5	38.0	32.0	38.0
42-43	35.896808510638294	38.0	36.0	38.0	32.0	38.0
44-45	35.810874704491724	38.0	36.0	38.0	31.5	38.0
46-47	35.84448384554767	38.0	36.0	38.0	31.5	38.0
48-49	35.84208037825059	38.0	36.0	38.0	32.0	38.0
50-51	35.8834121355398	38.0	36.0	38.0	32.0	38.0
52-53	35.85973207249803	38.0	36.5	38.0	32.0	38.0
54-55	35.79531126871552	38.0	36.0	38.0	31.5	38.0
56-57	35.763750985027585	38.0	36.0	38.0	31.5	38.0
58-59	35.74503546099291	38.0	36.0	38.0	31.0	38.0
60-61	35.75342789598109	38.0	36.0	38.0	31.0	38.0
62-63	35.68136327817179	38.0	36.0	38.0	31.0	38.0
64-65	35.67139479905437	38.0	36.0	38.0	31.0	38.0
66-67	35.63550039401103	38.0	36.0	38.0	31.0	38.0
68-69	35.52821118991332	38.0	36.0	38.0	31.0	38.0
70-71	35.37021276595745	38.0	36.0	38.0	30.0	38.0
72-73	35.489598108747046	38.0	36.0	38.0	31.0	38.0
74-75	35.4827817178881	38.0	36.0	38.0	31.0	38.0
76	34.36635145784082	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	1.0
22	1.0
23	1.0
24	3.0
25	14.0
26	22.0
27	57.0
28	68.0
29	136.0
30	217.0
31	344.0
32	585.0
33	884.0
34	1259.0
35	1805.0
36	2910.0
37	4380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46808510638298	21.07171000788022	11.536643026004729	22.92356185973207
2	24.94877856579984	21.37115839243499	33.57762017336485	20.102442868400317
3	22.789598108747043	24.649330181245073	29.79511426319937	22.76595744680851
4	17.163120567375888	18.400315208825845	37.738376674546885	26.69818754925138
5	16.80851063829787	35.1063829787234	34.05043341213554	14.034672970843184
6	33.018124507486206	35.83924349881797	17.84869976359338	13.293932230102442
7	31.66272655634358	28.778565799842397	22.84475965327029	16.713947990543733
8	27.257683215130022	34.8936170212766	19.82663514578408	18.0220646178093
9	27.84869976359338	18.817966903073284	21.300236406619387	32.03309692671395
10-11	28.140267927501974	25.275807722616232	26.97399527186761	19.609929078014186
12-13	28.06146572104019	22.38770685579196	26.871552403467298	22.67927501970055
14-15	26.4381402679275	24.33412135539795	23.400315208825848	25.8274231678487
16-17	26.481481481481485	27.915681639085893	24.692671394799053	20.91016548463357
18-19	25.886524822695034	26.993695823483055	25.717100078802208	21.4026792750197
20-21	24.393223010244284	27.39952718676123	25.57131599684791	22.63593380614657
22-23	25.858944050433415	26.56422379826635	26.237194641449964	21.339637509850277
24-25	25.752561071710005	27.23404255319149	26.587864460204884	20.425531914893615
26-27	25.08668242710796	28.31363278171789	26.710007880220648	19.889676910953508
28-29	24.732072498029943	26.595744680851062	28.38849487785658	20.28368794326241
30-31	25.40977147360126	25.429472025216704	28.455476753349096	20.70527974783294
32-33	23.93617021276596	27.442868400315206	27.64381402679275	20.977147360126082
34-35	25.070921985815602	26.12687155240347	27.828999211977933	20.973207249802993
36-37	25.78408195429472	26.851851851851855	27.143420015760437	20.220646178092984
38-39	25.066981875492512	26.583924349881798	27.608353033884946	20.74074074074074
40-41	24.91331757289204	25.03152088258471	28.975571315996845	21.0795902285264
42-43	25.354609929078016	25.724980299448386	28.703703703703702	20.216706067769895
44-45	25.137903861308114	26.710007880220648	27.698975571315998	20.45311268715524
46-47	25.996847911741526	25.319148936170212	27.624113475177303	21.059889676910952
48-49	25.137903861308114	25.2521670606777	27.907801418439714	21.70212765957447
50-51	24.436564223798264	25.98108747044917	28.447596532702917	21.134751773049647
52-53	24.75177304964539	25.3230890464933	29.590228526398736	20.33490937746257
54-55	24.822695035460992	25.587076438140265	29.09771473601261	20.49251379038613
56-57	25.03940110323089	25.65405831363278	28.246650906225373	21.059889676910952
58-59	25.070921985815602	26.639085894405046	27.85263987391647	20.437352245862883
60-61	25.043341213553976	26.997635933806148	27.671394799054372	20.2876280535855
62-63	23.6327817178881	28.14420803782506	27.631993695823482	20.591016548463354
64-65	23.861308116627267	29.22379826635146	26.489361702127663	20.425531914893615
66-67	24.04255319148936	28.904649330181243	26.851851851851855	20.20094562647754
68-69	23.01024428684003	29.676910953506695	27.249802994483847	20.063041765169427
70-71	23.947990543735227	28.557919621749413	26.481481481481485	21.012608353033883
72-73	24.152876280535853	27.143420015760437	27.206461780929864	21.49724192277384
74-75	23.63672182821119	28.384554767533494	27.064617809298657	20.91410559495666
76	23.814026792750198	27.90386130811663	27.927501970055165	20.354609929078013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	0.5
16	1.5
17	3.0
18	4.0
19	7.0
20	11.0
21	13.0
22	15.5
23	25.0
24	39.0
25	46.0
26	53.0
27	62.0
28	86.5
29	109.0
30	124.5
31	167.0
32	260.5
33	327.0
34	340.0
35	374.0
36	405.5
37	416.0
38	465.5
39	606.0
40	770.0
41	826.5
42	810.0
43	829.0
44	843.5
45	842.0
46	845.0
47	777.0
48	669.0
49	616.0
50	603.0
51	525.5
52	394.0
53	306.5
54	273.0
55	266.0
56	233.5
57	184.0
58	160.0
59	143.0
60	108.5
61	94.5
62	98.0
63	99.5
64	98.5
65	102.0
66	117.5
67	127.0
68	117.5
69	103.0
70	100.5
71	103.0
72	103.5
73	85.5
74	63.0
75	59.0
76	59.5
77	48.5
78	34.5
79	32.0
80	31.0
81	20.0
82	10.5
83	11.0
84	8.5
85	6.0
86	3.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	12690.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.90543735224585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.47390373125916	53.45941686367218
2	14.654492165483035	20.48857368006304
3	4.8359824146094015	10.141843971631205
4	2.0516289031676247	5.736800630417652
5	0.8454514710855596	2.955082742316785
6	0.46218013752677267	1.938534278959811
7	0.2705444707473791	1.3238770685579195
8	0.05636343140570398	0.31520882584712373
9	0.09018149024912638	0.5673758865248227
>10	0.24799909818509752	2.450748620961387
>50	0.011272686281140797	0.6225374310480694
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	79	0.6225374310480694	No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT	36	0.28368794326241137	RNA PCR Primer, Index 42 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	24	0.18912529550827423	No Hit
GTGTAGGAAGAGGCAGATAAAGAATATTGAGGCGCCATTGGCGTGAAGGT	22	0.17336485421591805	No Hit
GGGTGGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTG	19	0.14972419227738376	No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA	16	0.1260835303388495	No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT	16	0.1260835303388495	No Hit
GGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGGGATGGGTTCGA	14	0.11032308904649331	No Hit
GTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAATAAAA	13	0.1024428684003152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.007880220646178092	0.0
19	0.0	0.0	0.0	0.007880220646178092	0.0
20	0.0	0.0	0.0	0.015760441292356184	0.0
21	0.0	0.0	0.0	0.015760441292356184	0.0
22	0.0	0.0	0.0	0.015760441292356184	0.0
23	0.0	0.0	0.0	0.015760441292356184	0.0
24	0.0	0.0	0.0	0.015760441292356184	0.0
25	0.0	0.0	0.0	0.02364066193853428	0.0
26	0.0	0.0	0.0	0.02364066193853428	0.0
27	0.0	0.0	0.0	0.03152088258471237	0.0
28	0.0	0.0	0.0	0.039401103230890466	0.0
29	0.0	0.0	0.0	0.039401103230890466	0.0
30	0.0	0.0	0.0	0.039401103230890466	0.0
31	0.0	0.0	0.0	0.039401103230890466	0.0
32	0.0	0.0	0.0	0.039401103230890466	0.0
33	0.0	0.0	0.0	0.055161544523246654	0.0
34	0.0	0.0	0.0	0.1024428684003152	0.0
35	0.0	0.0	0.0	0.11032308904649331	0.0
36	0.0	0.0	0.0	0.12608353033884948	0.0
37	0.0	0.0	0.0	0.2285263987391647	0.0
38	0.0	0.0	0.0	0.2364066193853428	0.0
39	0.0	0.0	0.0	0.28368794326241137	0.0
40	0.0	0.0	0.0	0.3467297084318361	0.0
41	0.0	0.0	0.0	0.38613081166272656	0.0
42	0.0	0.0	0.0	0.48857368006304175	0.0
43	0.0	0.0	0.0	0.598896769109535	0.0
44	0.0	0.0	0.0	0.6934594168636722	0.0
45	0.0	0.0	0.0	0.8274231678486997	0.0
46	0.0	0.0	0.0	0.9377462568951931	0.0
47	0.0	0.0	0.0	1.095350669818755	0.0
48	0.0	0.0	0.0	1.2135539795114263	0.0
49	0.0	0.0	0.0	1.3238770685579195	0.0
50	0.0	0.0	0.0	1.4893617021276595	0.0
51	0.0	0.0	0.0	1.5839243498817968	0.0
52	0.0	0.0	0.0	1.7415287628053586	0.0
53	0.0	0.0	0.0	1.9148936170212767	0.0
54	0.0	0.0	0.0	2.0724980299448386	0.0
55	0.0	0.0	0.0	2.2379826635145785	0.0
56	0.0	0.0	0.0	2.4271079590228526	0.0
57	0.0	0.0	0.0	2.5847123719464147	0.0
58	0.0	0.0	0.0	2.710795902285264	0.0
59	0.0	0.0	0.0	2.9078014184397163	0.0
60	0.0	0.0	0.0	3.120567375886525	0.0
61	0.0	0.0	0.0	3.2781717888100865	0.0
62	0.0	0.0	0.0	3.4988179669030735	0.0
63	0.0	0.0	0.0	3.632781717888101	0.0
64	0.0	0.0	0.0	3.892828999211978	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTGAG	15	0.0021742468	70.00001	37
TAAGTAG	15	0.0021742468	70.00001	17
GAAAAAA	65	7.5303926E-4	26.923077	57
AAAAAAA	290	2.7927806E-4	10.862069	62
>>END_MODULE