##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779701_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11015 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.148978665456195 34.0 32.0 34.0 23.0 34.0 2 29.24838856105311 34.0 27.0 34.0 12.0 34.0 3 28.111393554244213 34.0 23.0 34.0 11.0 34.0 4 31.034044484793462 34.0 32.0 34.0 23.0 34.0 5 30.53599636858829 34.0 32.0 34.0 21.0 34.0 6 33.165592374035405 37.0 34.0 38.0 21.0 38.0 7 34.18819791193827 37.0 34.0 38.0 24.0 38.0 8 33.44611892873355 38.0 34.0 38.0 21.0 38.0 9 34.8531093962778 38.0 36.0 38.0 27.0 38.0 10-11 34.64108034498412 38.0 36.0 38.0 25.0 38.0 12-13 33.41688606445756 37.5 32.0 38.0 19.0 38.0 14-15 33.66754425783023 37.5 34.0 38.0 22.0 38.0 16-17 33.39568769859283 37.5 32.0 38.0 19.0 38.0 18-19 33.981525192918745 37.5 34.0 38.0 23.0 38.0 20-21 32.90753517930095 37.0 30.5 38.0 18.5 38.0 22-23 32.89400817067635 37.5 31.0 38.0 18.5 38.0 24-25 34.31343622333182 37.5 35.0 38.0 24.5 38.0 26-27 32.56640944167045 37.0 30.5 38.0 16.5 38.0 28-29 34.98733545165683 38.0 36.5 38.0 27.0 38.0 30-31 35.36745347253745 38.0 37.0 38.0 28.5 38.0 32-33 35.46536541080345 38.0 37.0 38.0 31.0 38.0 34-35 35.51398093508851 38.0 37.0 38.0 31.0 38.0 36-37 33.24625510667272 38.0 32.5 38.0 18.0 38.0 38-39 31.177848388561053 36.5 26.5 38.0 11.0 38.0 40-41 32.72074443940082 37.0 30.5 38.0 17.0 38.0 42-43 33.825873808443035 37.5 34.0 38.0 19.0 38.0 44-45 35.50821606899682 38.0 37.0 38.0 31.0 38.0 46-47 34.004493871992736 38.0 34.0 38.0 21.0 38.0 48-49 33.48620063549705 37.5 33.5 38.0 18.5 38.0 50-51 31.112301407172037 36.5 26.5 38.0 11.0 38.0 52-53 34.726463912846114 37.5 35.5 38.0 26.0 38.0 54-55 33.77734906945075 37.5 34.0 38.0 19.0 38.0 56-57 34.20290512936904 38.0 35.5 38.0 19.0 38.0 58-59 35.28111665910123 38.0 37.0 38.0 29.0 38.0 60-61 35.59605083976396 38.0 37.0 38.0 31.0 38.0 62-63 35.595823876532 38.0 37.0 38.0 30.0 38.0 64-65 35.33309123921924 38.0 37.0 38.0 28.0 38.0 66-67 33.172446663640486 37.5 34.0 38.0 15.5 38.0 68-69 33.29596005447117 37.0 34.0 38.0 16.0 38.0 70-71 32.623422605537904 37.0 32.0 38.0 16.0 38.0 72-73 31.395960054471175 36.5 26.5 38.0 11.0 38.0 74-75 33.97462551066727 37.5 35.0 38.0 24.0 38.0 76 34.56432137993645 37.0 36.0 38.0 25.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 11.0 14 18.0 15 47.0 16 59.0 17 79.0 18 113.0 19 107.0 20 95.0 21 95.0 22 96.0 23 81.0 24 88.0 25 92.0 26 111.0 27 150.0 28 155.0 29 212.0 30 226.0 31 349.0 32 512.0 33 803.0 34 1372.0 35 2060.0 36 2633.0 37 1450.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.31411711302769 21.407172038129822 12.691783931003178 29.586926917839314 2 19.909214707217433 20.898774398547435 36.250567408079895 22.941443486155244 3 19.94552882433046 19.845665002269634 33.890149795733095 26.318656377666816 4 12.619155696777124 17.84838856105311 34.807081252837044 34.72537448933273 5 12.201543349977303 31.493418066273264 38.90149795733091 17.403540626418522 6 24.16704493871993 35.4698138901498 19.128461189287336 21.23467998184294 7 23.876532001815708 30.276895142986838 21.452564684521107 24.39400817067635 8 21.280072628234226 32.87335451656831 19.836586472991375 26.009986382206087 9 22.35133908306854 17.203812982296867 20.880617339990923 39.56423059464367 10-11 21.983658647299137 25.533363595097597 27.680435769405353 24.802541988197913 12-13 22.48297775760327 22.128915115751248 28.99228325011348 26.395823876532003 14-15 21.27099409895597 24.198819791193827 25.197458011802087 29.332728098048115 16-17 21.470721743077622 26.336813436223334 27.099409895596914 25.09305492510213 18-19 19.61870177031321 27.317294598275076 27.598729005901045 25.46527462551067 20-21 21.166591012256013 26.4593735814798 27.93463458919655 24.439400817067636 22-23 21.62051747616886 25.379028597367224 28.1025873808443 24.89786654561961 24-25 19.455288243304587 27.385383567862004 26.967771221062186 26.191556967771223 26-27 19.79573309123922 26.087153881071266 29.092147072174306 25.024965955515206 28-29 19.931911030413076 27.757603268270543 27.948252383113935 24.36223331820245 30-31 20.09078529278257 27.743985474353156 27.13572401270994 25.029505220154334 32-33 19.78665456196096 27.330912392192463 27.862006354970493 25.02042669087608 34-35 20.490240581025873 27.562414888788016 27.040399455288245 24.906945074897866 36-37 20.34952337721289 26.486609169314573 29.82296867907399 23.340898774398546 38-39 20.739900136177937 24.970494779845666 32.21062187925556 22.078983204720835 40-41 19.986382206082613 25.583295506128007 26.631865637766683 27.798456650022697 42-43 18.34316840671811 26.772582841579663 27.90285973672265 26.981389014979573 44-45 18.93327280980481 28.002723558783476 27.64412165229233 25.419881979119385 46-47 20.358601906491145 26.25056740807989 28.85610531093963 24.534725374489334 48-49 20.358601906491145 27.38084430322288 28.47026781661371 23.790285973672265 50-51 21.652292328642762 25.451656831593283 30.20880617339991 22.687244666364048 52-53 19.08760780753518 27.671357240127097 28.41579664094417 24.825238311393555 54-55 20.79437131184748 27.153881071266454 27.816613708579208 24.235133908306857 56-57 20.68088969586927 27.176577394462097 27.762142532909667 24.380390376758964 58-59 19.555152065365412 27.812074443940084 27.562414888788016 25.070358601906488 60-61 19.24194280526555 27.66227871084884 28.002723558783476 25.09305492510213 62-63 18.393100317748527 27.285519745801178 28.924194280526557 25.397185655923742 64-65 19.037675896504766 28.461189287335454 27.802995914661828 24.698138901497956 66-67 18.679073990013617 26.27326373127553 25.746709033136632 29.300953245574217 68-69 18.11867253824851 27.01230308258047 26.808008353384484 28.061016025786532 70-71 18.597367226509306 25.982750794371313 25.75124829777576 29.66863368134362 72-73 18.039037675896505 25.3109396277803 25.44711756695415 31.20290512936904 74-75 18.92873354516568 27.703132092600995 27.557875624148885 25.81025873808443 76 18.683613254652744 27.889241942805263 27.19927371765774 26.227871084884246 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.5 17 5.0 18 4.5 19 5.5 20 6.0 21 5.0 22 9.5 23 18.5 24 27.5 25 32.0 26 40.0 27 62.0 28 89.5 29 103.0 30 123.5 31 153.0 32 186.5 33 211.0 34 238.0 35 291.0 36 363.5 37 410.0 38 453.0 39 556.0 40 637.5 41 652.5 42 646.0 43 673.0 44 716.5 45 709.5 46 686.0 47 678.5 48 637.5 49 581.0 50 558.0 51 519.5 52 431.5 53 360.0 54 338.0 55 294.5 56 228.5 57 181.0 58 156.0 59 152.0 60 124.5 61 107.0 62 113.0 63 99.5 64 84.5 65 79.5 66 74.0 67 72.0 68 59.5 69 45.0 70 44.5 71 46.0 72 46.0 73 37.0 74 26.5 75 25.0 76 23.0 77 19.5 78 14.0 79 10.0 80 10.5 81 9.5 82 9.0 83 10.0 84 7.5 85 5.5 86 4.0 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.013617793917385383 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 11015.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.55787562414889 #Duplication Level Percentage of deduplicated Percentage of total 1 97.95272659594268 95.56059918293236 2 1.7587939698492463 3.4316840671811164 3 0.18611576400521124 0.5447117566954154 4 0.0744463056020845 0.2905129369042215 5 0.009305788200260562 0.04539264639128461 6 0.009305788200260562 0.05447117566954153 7 0.0 0.0 8 0.009305788200260562 0.07262823422605537 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.018157058556513846 0.0 20 0.0 0.0 0.0 0.02723558783477077 0.0 21 0.0 0.0 0.0 0.02723558783477077 0.0 22 0.0 0.0 0.0 0.05447117566954154 0.0 23 0.0 0.0 0.0 0.05447117566954154 0.0 24 0.0 0.0 0.0 0.05447117566954154 0.0 25 0.0 0.0 0.0 0.05447117566954154 0.0 26 0.0 0.0 0.0 0.05447117566954154 0.0 27 0.0 0.0 0.0 0.05447117566954154 0.0 28 0.0 0.0 0.0 0.05447117566954154 0.0 29 0.0 0.0 0.0 0.05447117566954154 0.0 30 0.0 0.0 0.0 0.05447117566954154 0.0 31 0.0 0.0 0.0 0.05447117566954154 0.0 32 0.0 0.0 0.0 0.05447117566954154 0.0 33 0.0 0.0 0.0 0.06354970494779846 0.0 34 0.0 0.0 0.0 0.06354970494779846 0.0 35 0.0 0.0 0.0 0.07262823422605538 0.0 36 0.0 0.0 0.0 0.07262823422605538 0.0 37 0.0 0.0 0.0 0.09078529278256922 0.0 38 0.0 0.0 0.0 0.11802088061734 0.0 39 0.0 0.0 0.0 0.17249205628688152 0.0 40 0.0 0.0 0.0 0.21788470267816615 0.0 41 0.0 0.0 0.0 0.30866999546073537 0.0 42 0.0 0.0 0.0 0.39037675896504764 0.0 43 0.0 0.0 0.0 0.46300499319110305 0.0 44 0.0 0.0 0.0 0.5719473445301861 0.0 45 0.0 0.0 0.0 0.6173399909214707 0.0 46 0.0 0.0 0.0 0.6445755787562415 0.0 47 0.0 0.0 0.0 0.7262823422605538 0.0 48 0.0 0.0 0.0 0.7807535179300953 0.0 49 0.0 0.0 0.0 0.8261461643213799 0.0 50 0.0 0.0 0.0 0.9714026327734907 0.0 51 0.0 0.0 0.0 1.0894235133908308 0.0 52 0.0 0.0 0.0 1.1802088061734 0.0 53 0.0 0.0 0.0 1.3617793917385383 0.0 54 0.0 0.0 0.0 1.4798002723558783 0.0 55 0.0 0.0 0.0 1.5978211529732183 0.0 56 0.0 0.0 0.0 1.7067635043123015 0.0 57 0.0 0.0 0.0 1.7975487970948707 0.0 58 0.0 0.0 0.0 1.8429414434861553 0.0 59 0.0 0.0 0.0 1.88833408987744 0.0 60 0.0 0.0 0.0 2.00635497049478 0.0 61 0.0 0.0 0.0 2.1516114389468908 0.0 62 0.0 0.0 0.0 2.1788470267816615 0.0 63 0.0 0.0 0.0 2.2877893781207446 0.0 64 0.0 0.0 0.0 2.333182024512029 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGAC 15 0.0021705348 69.96818 19 CATCCAT 15 0.0021705348 69.96818 38 GCCGACG 15 0.0021705348 69.96818 20 CTGCCGA 20 0.0067834947 52.476135 18 >>END_MODULE