FastQCFastQC Report
Wed 25 May 2016
SRR1779701_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779701_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11015
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT850.7716749886518384No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT380.34498411257376305RNA PCR Primer, Index 15 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC250.22696323195642307No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC190.17249205628688152No Hit
ATTATGTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAG130.11802088061733998No Hit
GGGTGGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTG120.10894235133908306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC150.002166624270.03
TGCCGTC350.001202341840.045
CCGTCTT350.001202341840.047
TTGAAAA350.001202341840.058
GCCGTCT350.001202341840.046
CGTCTTC350.001202341840.048
TATGCCG400.002317103835.00000443
TGCTTGA400.002317103835.00000455
ATGCCGT400.002317103835.00000444
GTCTTCT400.002317103835.00000449
CGTATGC400.002317103835.00000441
CTGCTTG400.002317103835.00000454
TCGTATG400.002317103835.00000440
TCTTCTG400.002317103835.00000450
CCGAGCC552.806426E-431.8181814
GTATGCC450.00412731431.11111342
TCTGCTT450.00412731431.11111353
GCTTGAA450.00412731431.11111356
CTTGAAA450.00412731431.11111357
ATCTCCG604.6743767E-429.16666610