FastQCFastQC Report
Wed 25 May 2016
SRR1779700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779700_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT933.4355374953823423No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT421.551533062430735Illumina PCR Primer Index 9 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC170.6280014776505356No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC170.6280014776505356No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG150.5541189508681197No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC90.33247137052087183No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC70.25858884373845586No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG60.2216475803472479No Hit
CTTATAAACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT60.2216475803472479No Hit
CCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAA50.1847063169560399No Hit
CACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAAC50.1847063169560399No Hit
GTACTATTTATACTAAAAGAGTAAGACCCTCATCAATAGATGGAGACATA40.1477650535648319No Hit
CACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACCACCTA40.1477650535648319No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT40.1477650535648319TruSeq Adapter, Index 12 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT40.1477650535648319Illumina PCR Primer Index 10 (95% over 23bp)
GTAGATGCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTT40.1477650535648319No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG40.1477650535648319No Hit
GAGTAGGGGAAGGGAGCCTACTAGGGTGTAGAATAGGAAGTATGTGCCTG40.1477650535648319No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT40.1477650535648319No Hit
GAGCAAGAACTGAGCCAAAATTGTCCGGTAGGAGCACTCCGTATTAGGTG40.1477650535648319No Hit
GGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGATGGG40.1477650535648319No Hit
GTATAAGAGACAGGCAGCGTCAGATGTGTATAAGAGCTGTCTCTTATACA40.1477650535648319No Hit
GGTAAAGCTGCCTGGAATAGATGAGTTTGTATCTATGTTAATGCTGATGG40.1477650535648319No Hit
GCAGCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCAC40.1477650535648319No Hit
GGAATGGAATGGAAACAACACGAATGGAATGGAACGGAATGGAATGGAAT30.11082379017362395No Hit
GATAAGGAGACACCAGCAAAGGAGACTGAAGAGGAGTGAGCAGTAACGGA30.11082379017362395No Hit
ATTAAACACAAACTACCACCTACCTCCCTCACCAAAGCCCATAAAAATAA30.11082379017362395No Hit
ACCAAATCTCCACCTCCATCATCACCTCAACCCAAAAAGGCATAATTAAA30.11082379017362395No Hit
CCTGGATGGACAGCGGAAGAAGCGAGGCGGCCGCAGGTGAGGGGCCCTGG30.11082379017362395No Hit
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA30.11082379017362395No Hit
GACAGGCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCC30.11082379017362395No Hit
ATTGAGGCCCAGCCTGTCTGGCACTAAAGTCCTAGTCTTTTGGATTTCAT30.11082379017362395No Hit
ATGTTAGCGGTTAGGCGTACGGCCAGGGCTATTGGTTGAATGAGTAGGCT30.11082379017362395No Hit
CAGTTGATACATAGCGGGATCAACGCAGATCTAGATAAGACCAGTCTTAG30.11082379017362395No Hit
AAGGAGGTTTATGTTAGGAACTTTCTTTCAATAAAGCAATTTAGGACATA30.11082379017362395No Hit
TATATATTGTAATTGAGATTGCTCGGGGGAATAGGTTATGTGATTAGGAG30.11082379017362395No Hit
AGCCGACCCAGCGCGAGCAGAAAGCCGACTACGCCGAAGAACCAGACGAA30.11082379017362395No Hit
CTATTGTGTAAGAGACTAGAGGAGCTTAATCAAGTTAGTTGCTATTTAAA30.11082379017362395No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT30.11082379017362395TruSeq Adapter, Index 2 (95% over 21bp)
ATACTAGCCAGGCTGGTCTCGAACTCCTAACCTTGTGATCTGCCCACCTC30.11082379017362395No Hit
GTGTATAAGAGACAGGCGTCAGATGTGTATAAGAGACAGCTCTTATACAC30.11082379017362395No Hit
GATCACAGGCATGACCCACCATGCCTGGACTTCATTTTTTTTAATTTCAA30.11082379017362395No Hit
ATATCAGATTGTGTTAGAGTTTCTATTTAAATCATCAAATATGATTTTAA30.11082379017362395No Hit
GCAGCGTCAGATGTGTATAAGAGACAGCTCTTATACACATCTCCGAGCCC30.11082379017362395No Hit
GTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCT30.11082379017362395No Hit
AACGCGGAGGGCGGCGGCCCGGAGAACATGCTCCGCAACCTGCGCGAGCT30.11082379017362395No Hit
CCTCTCAGAGATGTAATGAATAAATCTGAATGCTTCTCCCTTTATCCTGT30.11082379017362395No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC30.11082379017362395No Hit
ACCCATACTGAGACGCAGAACCCGATCACCTGGCAGGCAGCCACCGGGCA30.11082379017362395No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGTGATGACCCCGGTGCTGGCCAC30.11082379017362395No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC30.11082379017362395No Hit
TTTTTGAATGTTGTCAAAACTAGTTAATTGGAAGTTAACGGTACTATTTA30.11082379017362395No Hit
GCGACGAAGTCGGCGGCGACCAAGACCGCTGCCACGCCGACGGCCAAGAA30.11082379017362395No Hit
ATTGAAGAGTATGCAATGAGCGATTTTAGGTCTGTTTGTCGTAGGCAGAT30.11082379017362395No Hit
GGACTTCACCAGGGCGGGCGCGGTGGCCTTCCACTGCGACCGGCGGGCAC30.11082379017362395No Hit
GTATAAGAGACAGCGGTAACTAAGATTAGTATGGTAATTAGGAAGATGAG30.11082379017362395No Hit
CTTATATACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT30.11082379017362395No Hit
TTCCATTCCATTGCACTCCATTCCATTCCTTTGCACTCGTGTTGATTCCA30.11082379017362395No Hit
GGGGTTCATAGTAGAAGAGCGATGGTGAGAGCTAAGGTCGGGGCGGTGAT30.11082379017362395No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC30.11082379017362395RNA PCR Primer, Index 48 (95% over 21bp)
CTAAATGTTTTAAAGTAGTCATGTTTGTGTTTGAGAAAGGTACCTTTAGC30.11082379017362395No Hit
GCAGCGTCAGATGTGTATAAGAGACAGTTTTTATTGTTGCTGTTTGTTTG30.11082379017362395No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG30.11082379017362395No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT30.11082379017362395RNA PCR Primer, Index 30 (95% over 22bp)
TGTTGAATAACAGCTGAAAAAAACTAACTGGGTGTGTGAGGGGCTGCTGT30.11082379017362395No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG450.00359748131.11110937
ATCTCGT550.00960228325.45454438
TCTCGTA550.00960228325.45454439
TGCTTGA550.00960228325.45454456
GCTTGAA550.00960228325.45454457
TTGAAAA550.00960228325.45454459
AAAAAAA3700.00147500758.51351462
Produced by FastQC (version 0.11.5)