##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779699_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1860
Sequences flagged as poor quality	0
Sequence length	76
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.203225806451613	33.0	23.0	34.0	12.0	34.0
2	27.205376344086023	32.0	23.0	34.0	12.0	34.0
3	26.573118279569893	32.0	21.0	34.0	11.0	34.0
4	28.397849462365592	34.0	23.0	34.0	12.0	34.0
5	27.81290322580645	34.0	23.0	34.0	11.0	34.0
6	29.741397849462366	35.0	23.0	38.0	11.0	38.0
7	31.195698924731182	37.0	26.0	38.0	12.0	38.0
8	30.239247311827956	36.0	24.0	38.0	11.0	38.0
9	32.026881720430104	37.0	28.0	38.0	12.0	38.0
10-11	31.388172043010755	37.0	27.0	38.0	11.0	38.0
12-13	30.627150537634407	35.5	27.0	38.0	11.0	38.0
14-15	30.421505376344086	36.0	24.5	38.0	11.0	38.0
16-17	30.352150537634408	35.5	25.0	38.0	11.0	38.0
18-19	30.843548387096774	36.5	26.0	38.0	11.5	38.0
20-21	29.740860215053765	35.5	23.5	38.0	11.0	38.0
22-23	30.1247311827957	35.5	24.5	38.0	11.0	38.0
24-25	31.104032258064514	36.5	26.5	38.0	11.0	38.0
26-27	29.508602150537634	35.0	22.0	38.0	11.0	38.0
28-29	31.29462365591398	37.0	26.5	38.0	11.0	38.0
30-31	31.84193548387097	37.5	28.5	38.0	11.0	38.0
32-33	32.013172043010755	38.0	31.0	38.0	11.0	38.0
34-35	32.05241935483871	38.0	31.0	38.0	11.0	38.0
36-37	30.218548387096774	36.5	24.0	38.0	11.0	38.0
38-39	28.77768817204301	34.0	21.5	37.5	11.0	38.0
40-41	29.21478494623656	34.0	22.0	37.5	11.0	38.0
42-43	30.51102150537634	36.5	25.0	38.0	11.0	38.0
44-45	31.747580645161293	37.0	27.0	38.0	11.0	38.0
46-47	30.591397849462368	36.0	24.5	38.0	11.0	38.0
48-49	29.916129032258063	35.5	23.5	38.0	11.0	38.0
50-51	27.976881720430107	33.5	21.0	37.5	11.0	38.0
52-53	30.92231182795699	36.5	26.0	38.0	11.0	38.0
54-55	29.9997311827957	35.5	24.0	38.0	11.0	38.0
56-57	30.425806451612903	36.5	24.5	38.0	11.0	38.0
58-59	31.398118279569893	37.0	26.5	38.0	11.0	38.0
60-61	31.943279569892475	38.0	29.0	38.0	11.0	38.0
62-63	31.987903225806452	37.5	29.0	38.0	11.0	38.0
64-65	31.7497311827957	37.5	27.5	38.0	11.0	38.0
66-67	29.803494623655915	35.5	23.5	38.0	11.0	38.0
68-69	29.836559139784946	36.0	23.0	38.0	11.0	38.0
70-71	29.313709677419354	35.0	22.5	38.0	11.0	38.0
72-73	28.326075268817206	34.0	21.0	37.5	11.0	38.0
74-75	30.07715053763441	35.5	24.0	38.0	11.0	38.0
76	30.259677419354837	36.0	24.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	8.0
14	10.0
15	36.0
16	32.0
17	52.0
18	61.0
19	71.0
20	55.0
21	65.0
22	34.0
23	23.0
24	35.0
25	29.0
26	42.0
27	31.0
28	39.0
29	28.0
30	59.0
31	68.0
32	67.0
33	137.0
34	165.0
35	247.0
36	315.0
37	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.881720430107528	19.193548387096772	12.956989247311828	35.96774193548387
2	20.161290322580644	15.053763440860216	39.247311827956985	25.537634408602152
3	21.29032258064516	14.946236559139786	35.43010752688172	28.333333333333332
4	15.96774193548387	18.225806451612904	28.763440860215056	37.04301075268817
5	16.612903225806452	24.247311827956988	36.98924731182795	22.1505376344086
6	27.956989247311824	32.903225806451616	13.38709677419355	25.75268817204301
7	26.82795698924731	24.946236559139784	17.634408602150536	30.591397849462364
8	22.795698924731184	32.795698924731184	16.881720430107528	27.526881720430108
9	21.29032258064516	14.89247311827957	19.408602150537636	44.40860215053763
10-11	24.56989247311828	21.236559139784948	26.801075268817204	27.392473118279568
12-13	23.655913978494624	17.446236559139784	28.655913978494624	30.241935483870968
14-15	24.327956989247312	21.344086021505376	22.822580645161292	31.505376344086024
16-17	25.967741935483872	20.188172043010752	24.327956989247312	29.516129032258064
18-19	22.63440860215054	20.161290322580644	27.688172043010752	29.516129032258064
20-21	26.263440860215052	19.946236559139784	25.483870967741932	28.306451612903228
22-23	26.666666666666668	19.35483870967742	24.677419354838708	29.301075268817208
24-25	22.903225806451612	22.876344086021504	21.20967741935484	33.01075268817204
26-27	24.838709677419356	22.526881720430108	25.376344086021508	27.258064516129032
28-29	24.48924731182796	21.397849462365592	24.56989247311828	29.54301075268817
30-31	23.225806451612904	23.010752688172044	24.032258064516128	29.731182795698924
32-33	23.306451612903224	22.043010752688172	24.704301075268816	29.946236559139784
34-35	23.844086021505376	22.93010752688172	22.526881720430108	30.698924731182796
36-37	24.704301075268816	20.7258064516129	28.763440860215056	25.806451612903224
38-39	25.134408602150536	18.064516129032256	32.93010752688172	23.870967741935484
40-41	25.026881720430104	21.774193548387096	23.172043010752688	30.02688172043011
42-43	22.016129032258064	22.983870967741936	25.053763440860216	29.946236559139784
44-45	20.887096774193548	20.77956989247312	27.365591397849464	30.967741935483872
46-47	24.193548387096776	21.155913978494624	26.344086021505376	28.306451612903228
48-49	25.403225806451612	21.20967741935484	26.424731182795696	26.96236559139785
50-51	29.408602150537632	19.301075268817204	27.500000000000004	23.790322580645164
52-53	20.376344086021504	20.7258064516129	28.413978494623652	30.483870967741932
54-55	25.403225806451612	21.182795698924732	23.306451612903224	30.107526881720432
56-57	25.430107526881716	21.29032258064516	24.543010752688172	28.736559139784944
58-59	21.102150537634408	24.731182795698924	24.6505376344086	29.516129032258064
60-61	23.091397849462368	20.026881720430108	25.349462365591396	31.532258064516128
62-63	21.263440860215056	22.661290322580648	25.349462365591396	30.725806451612904
64-65	21.344086021505376	24.35483870967742	24.35483870967742	29.946236559139784
66-67	20.56451612903226	27.043010752688172	20.483870967741936	31.908602150537636
68-69	21.532258064516128	26.63978494623656	20.698924731182796	31.129032258064516
70-71	20.080645161290324	25.99462365591398	21.048387096774192	32.876344086021504
72-73	18.629032258064516	26.129032258064516	21.021505376344084	34.22043010752688
74-75	20.77956989247312	25.913978494623656	21.908602150537636	31.397849462365592
76	19.946236559139784	24.89247311827957	22.419354838709676	32.74193548387097
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	2.0
21	2.0
22	4.5
23	7.5
24	8.5
25	9.0
26	10.5
27	11.5
28	10.5
29	10.0
30	13.5
31	21.5
32	27.5
33	29.0
34	31.5
35	35.5
36	40.0
37	43.0
38	47.0
39	52.5
40	52.5
41	57.0
42	63.0
43	66.0
44	61.5
45	59.5
46	65.0
47	70.5
48	68.0
49	57.0
50	54.0
51	56.0
52	62.5
53	70.0
54	73.0
55	56.0
56	47.0
57	53.5
58	52.0
59	48.0
60	47.5
61	54.0
62	57.0
63	45.5
64	45.5
65	50.0
66	52.5
67	62.0
68	54.0
69	45.0
70	41.5
71	39.0
72	44.0
73	41.5
74	34.5
75	35.0
76	29.0
77	22.5
78	16.5
79	11.0
80	10.0
81	7.0
82	4.0
83	3.0
84	3.0
85	2.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	1860.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.6236559139785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.62223421478683	99.24731182795699
2	0.37776578521316784	0.7526881720430108
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCCGATCTTGGTGATGCGGGTGTAGCCGCCGTTGCGCTCGGCCATCGCG	2	0.10752688172043011	No Hit
CCCTTGGGTTTCTAAAGTCTATAAAAAGCACTAAGTGAAGATAATAGCAA	2	0.10752688172043011	No Hit
GTGTGCCGGAAGCTCGTCCAGTCGGGACGCAGGGCCACCACCAGCGTGTC	2	0.10752688172043011	No Hit
GCTCCACGGACCGTCCCGGTCCGCGATCCTCGAGGTCGCGGCTGTCTCTT	2	0.10752688172043011	No Hit
GCATTTAAACAAGTGTTCAAAGTTTACATAGCTAATATGAGACAAATGAT	2	0.10752688172043011	No Hit
ACATTACAGCAACATTTGCTAGAGGTAGAAAAATTAACATTAAGTAAATA	2	0.10752688172043011	No Hit
GCCACTGGAGGCAAACACACGCAGAGCCCAGAGACAGTGGAGGAGCTGCT	2	0.10752688172043011	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.053763440860215055	0.0
15	0.0	0.0	0.0	0.053763440860215055	0.0
16	0.0	0.0	0.0	0.053763440860215055	0.0
17	0.0	0.0	0.0	0.053763440860215055	0.0
18	0.0	0.0	0.0	0.053763440860215055	0.0
19	0.0	0.0	0.0	0.053763440860215055	0.0
20	0.0	0.0	0.0	0.053763440860215055	0.0
21	0.0	0.0	0.0	0.053763440860215055	0.0
22	0.0	0.0	0.0	0.16129032258064516	0.0
23	0.0	0.0	0.0	0.21505376344086022	0.0
24	0.0	0.0	0.0	0.21505376344086022	0.0
25	0.0	0.0	0.0	0.21505376344086022	0.0
26	0.0	0.0	0.0	0.21505376344086022	0.0
27	0.0	0.0	0.0	0.21505376344086022	0.0
28	0.0	0.0	0.0	0.21505376344086022	0.0
29	0.0	0.0	0.0	0.21505376344086022	0.0
30	0.0	0.0	0.0	0.21505376344086022	0.0
31	0.0	0.0	0.0	0.21505376344086022	0.0
32	0.0	0.0	0.0	0.26881720430107525	0.0
33	0.0	0.0	0.0	0.26881720430107525	0.0
34	0.0	0.0	0.0	0.26881720430107525	0.0
35	0.0	0.0	0.0	0.4838709677419355	0.0
36	0.0	0.0	0.0	0.5376344086021505	0.0
37	0.0	0.0	0.0	0.6451612903225806	0.0
38	0.0	0.0	0.0	0.7526881720430108	0.0
39	0.0	0.0	0.0	0.8064516129032258	0.0
40	0.0	0.0	0.0	0.9139784946236559	0.0
41	0.0	0.0	0.0	1.075268817204301	0.0
42	0.0	0.0	0.0	1.2365591397849462	0.0
43	0.0	0.0	0.0	1.5591397849462365	0.0
44	0.0	0.0	0.0	1.7204301075268817	0.0
45	0.0	0.0	0.0	1.8279569892473118	0.0
46	0.0	0.0	0.0	1.935483870967742	0.0
47	0.0	0.0	0.0	1.989247311827957	0.0
48	0.0	0.0	0.0	2.043010752688172	0.0
49	0.0	0.0	0.0	2.2580645161290325	0.0
50	0.0	0.0	0.0	2.3118279569892475	0.0
51	0.0	0.0	0.0	2.3118279569892475	0.0
52	0.0	0.0	0.0	2.3118279569892475	0.0
53	0.0	0.0	0.0	2.4731182795698925	0.0
54	0.0	0.0	0.0	2.6344086021505375	0.0
55	0.0	0.0	0.0	2.849462365591398	0.0
56	0.0	0.0	0.0	2.903225806451613	0.0
57	0.0	0.0	0.0	3.118279569892473	0.0
58	0.0	0.0	0.0	3.172043010752688	0.0
59	0.0	0.0	0.0	3.3333333333333335	0.0
60	0.0	0.0	0.0	3.4946236559139785	0.0
61	0.0	0.0	0.0	3.4946236559139785	0.0
62	0.0	0.0	0.0	3.6559139784946235	0.0
63	0.0	0.0	0.0	3.6559139784946235	0.0
64	0.0	0.0	0.0	3.7096774193548385	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE