##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779698_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18149
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.82235935864235	34.0	32.0	34.0	21.0	34.0
2	28.87123257479751	34.0	24.0	34.0	12.0	34.0
3	27.792109758113394	34.0	23.0	34.0	11.0	34.0
4	30.695410215438866	34.0	32.0	34.0	23.0	34.0
5	30.15549066064246	34.0	31.0	34.0	11.0	34.0
6	32.74323654195823	37.0	33.0	38.0	12.0	38.0
7	33.61446911675574	37.0	34.0	38.0	21.0	38.0
8	33.050856796517714	37.0	34.0	38.0	12.0	38.0
9	34.5259242933495	38.0	34.0	38.0	26.0	38.0
10-11	34.38845115433357	38.0	35.0	38.0	24.0	38.0
12-13	33.102732932943965	37.5	31.5	38.0	19.0	38.0
14-15	33.360653479530555	37.0	33.5	38.0	21.0	38.0
16-17	32.87296820761475	37.5	31.5	38.0	18.5	38.0
18-19	33.61119069921208	37.0	34.0	38.0	22.0	38.0
20-21	32.47779491983029	37.0	30.5	38.0	17.5	38.0
22-23	32.37588847870406	37.0	29.5	38.0	18.0	38.0
24-25	33.9736900104689	37.0	34.5	38.0	24.0	38.0
26-27	32.118353628299076	37.0	30.5	38.0	16.0	38.0
28-29	34.70979117306739	37.5	35.0	38.0	26.5	38.0
30-31	35.22568736569508	38.0	36.5	38.0	27.0	38.0
32-33	35.248223042591874	38.0	37.0	38.0	27.0	38.0
34-35	35.30974158355832	38.0	37.0	38.0	27.0	38.0
36-37	32.880874979337705	37.0	30.5	38.0	17.0	38.0
38-39	30.6865943027164	36.5	26.0	38.0	11.0	38.0
40-41	32.316794313736295	36.5	30.5	38.0	16.0	38.0
42-43	33.54892831560967	37.0	33.0	38.0	18.5	38.0
44-45	35.26566201994601	38.0	36.5	38.0	28.0	38.0
46-47	33.72271199515125	37.5	32.0	38.0	21.0	38.0
48-49	33.182819990082095	37.0	32.0	38.0	18.5	38.0
50-51	30.521598986170037	36.0	25.0	38.0	11.0	38.0
52-53	34.4640476059287	37.0	35.0	38.0	25.5	38.0
54-55	33.49286462064026	37.5	33.0	38.0	18.5	38.0
56-57	33.93655297812552	37.5	34.0	38.0	19.0	38.0
58-59	35.13295498374566	38.0	36.5	38.0	28.0	38.0
60-61	35.52909251198413	38.0	37.0	38.0	31.0	38.0
62-63	35.40845225632266	38.0	37.0	38.0	29.0	38.0
64-65	35.15419582346135	38.0	36.5	38.0	27.5	38.0
66-67	32.767783348944846	37.5	32.5	38.0	11.0	38.0
68-69	32.7729626976693	37.0	32.5	38.0	16.0	38.0
70-71	32.255936966224034	37.0	30.0	38.0	15.5	38.0
72-73	30.89925064741859	36.5	26.0	38.0	11.0	38.0
74-75	33.65022866273624	37.0	33.0	38.0	24.0	38.0
76	34.2551104744063	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	10.0
14	41.0
15	80.0
16	85.0
17	129.0
18	168.0
19	159.0
20	164.0
21	135.0
22	145.0
23	160.0
24	147.0
25	188.0
26	220.0
27	239.0
28	310.0
29	413.0
30	553.0
31	766.0
32	1097.0
33	1732.0
34	2487.0
35	3289.0
36	3684.0
37	1748.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.81756570609951	19.455617389387843	14.380957628519479	27.345859275993167
2	19.885393134607966	19.747644498319467	37.51170863408452	22.855253732988043
3	20.01763182544493	20.09477106176649	34.86693481734531	25.020662295443273
4	12.689404374896688	18.403217808143697	36.84500523444818	32.062372582511436
5	11.774753429941045	32.238690836960714	40.21709185079067	15.769463882307566
6	26.199790622072843	35.75954598049479	18.078131026502838	19.962532370929527
7	25.918783404044298	28.640696457105076	22.348338751446363	23.092181387404263
8	21.703675133616176	34.38756956306133	19.681525152900985	24.227230150421512
9	22.68995536944184	16.788803790842472	21.025951843076754	39.495288996638934
10-11	23.296049369111245	24.957297922750566	28.249490330045735	23.497162378092458
12-13	22.758829687586093	22.10039120612706	30.059507410876634	25.08127169541022
14-15	22.031516887982807	24.11427626866494	24.57160174114276	29.282605102209487
16-17	22.871783569342664	25.8085844950135	27.632376439473248	23.687255496170586
18-19	20.9570775249325	26.57446691277756	28.155821257369553	24.31263430492038
20-21	22.43374290594523	25.513802413356107	27.976748030194504	24.075706650504163
22-23	22.877293514794204	24.59364152294892	28.87486913879553	23.654195823461347
24-25	20.053446470879937	27.486362885007438	26.995977739820376	25.46421290429225
26-27	20.640255661468952	26.265909967491325	29.089757011405588	24.00407735963414
28-29	20.4887321615516	27.070362003416164	28.621411648024687	23.819494187007546
30-31	20.58515620695355	27.180560912446968	28.571822138960822	23.662460741638657
32-33	20.53556669788969	27.822469557551383	27.310044630558156	24.331919114000772
34-35	21.463992506474185	26.560692049148717	27.635131412199023	24.34018403217808
36-37	22.09488126067552	25.263099895311036	29.946553529120063	22.695465314893383
38-39	21.623780924568848	25.026172240894816	32.690506363987	20.65954047054934
40-41	20.731169761419363	25.74797509504656	26.849964185354562	26.670890958179516
42-43	19.684280125626756	27.070362003416164	27.092401785222325	26.15295608573475
44-45	19.824783734641027	27.387183866879717	27.943688357485264	24.84434404099399
46-47	21.202270097526036	25.896743622238137	29.089757011405588	23.81122926883024
48-49	21.687145297261555	26.579976858229102	28.574577111686594	23.158300732822745
50-51	22.965452642018843	24.786489613752824	30.373574301614415	21.87448344261392
52-53	20.62923577056587	26.880268885338037	28.235715466416885	24.254779877679212
54-55	21.312469006556835	26.582731830954877	28.536007493525815	23.568791668962476
56-57	20.88820320678825	26.533142321891013	27.73155545760097	24.847099013719763
58-59	20.565871397873163	27.268720039671607	27.81695961209984	24.34844895035539
60-61	20.02865171634801	27.130971403383107	28.365199184528073	24.47517769574081
62-63	19.188385034988155	27.52217753044245	27.93542343930795	25.354013995261447
64-65	20.794534134112073	28.114496666483003	27.285249876026228	23.8057193233787
66-67	19.27929913493856	26.560692049148717	25.76725990412695	28.392748911785777
68-69	18.15577044934843	27.297021792434638	26.988456346254512	27.558751411962422
70-71	18.981762080555402	26.910573585321508	26.22458537660477	27.88307895751832
72-73	18.75309934431649	25.80307454956196	25.163920877183315	30.27990522893823
74-75	19.441842525758997	28.10347677557992	27.246680257865446	25.208000440795637
76	19.753154443771006	27.924403548404868	27.68747589398865	24.634966113835475
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.5
17	2.0
18	2.5
19	4.5
20	6.5
21	7.0
22	10.5
23	23.5
24	41.0
25	49.0
26	49.0
27	69.5
28	111.0
29	132.0
30	156.5
31	232.0
32	307.5
33	332.0
34	392.0
35	500.0
36	621.0
37	694.0
38	754.5
39	890.5
40	1002.0
41	1092.5
42	1147.0
43	1181.0
44	1227.5
45	1226.0
46	1212.0
47	1169.0
48	1086.5
49	988.0
50	929.0
51	881.5
52	755.5
53	615.0
54	553.0
55	503.0
56	387.0
57	281.0
58	241.0
59	229.5
60	181.0
61	138.0
62	132.0
63	118.5
64	101.5
65	93.0
66	92.5
67	97.0
68	90.5
69	87.5
70	83.0
71	75.0
72	76.0
73	78.0
74	61.5
75	44.0
76	37.5
77	30.5
78	24.0
79	18.0
80	17.0
81	14.5
82	10.0
83	7.0
84	5.5
85	4.5
86	3.0
87	1.0
88	0.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.002754972725770015
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	18149.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.87586092897679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.4633147537254	94.41842525758996
2	2.0589238994426116	3.9892005069149814
3	0.3469457399613241	1.0083200176318254
4	0.08531452621999773	0.3305967270924018
5	0.02843817540666591	0.13774863628850076
6	0.005687635081333181	0.03305967270924018
7	0.005687635081333181	0.03856961816078021
8	0.005687635081333181	0.04407956361232024
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.01101989090308006	0.0
10	0.0	0.0	0.0	0.01101989090308006	0.0
11	0.0	0.0	0.0	0.01101989090308006	0.0
12	0.0	0.0	0.0	0.01101989090308006	0.0
13	0.0	0.0	0.0	0.01101989090308006	0.0
14	0.0	0.0	0.0	0.01101989090308006	0.0
15	0.0	0.0	0.0	0.01101989090308006	0.0
16	0.0	0.0	0.0	0.01101989090308006	0.0
17	0.0	0.0	0.0	0.01101989090308006	0.0
18	0.0	0.0	0.0	0.01101989090308006	0.0
19	0.0	0.0	0.0	0.01652983635462009	0.0
20	0.0	0.0	0.0	0.01652983635462009	0.0
21	0.0	0.0	0.0	0.01652983635462009	0.0
22	0.0	0.0	0.0	0.02754972725770015	0.0
23	0.0	0.0	0.0	0.02754972725770015	0.0
24	0.0	0.0	0.0	0.03856961816078021	0.0
25	0.0	0.0	0.0	0.04407956361232024	0.0
26	0.0	0.0	0.0	0.04407956361232024	0.0
27	0.0	0.0	0.0	0.04407956361232024	0.0
28	0.0	0.0	0.0	0.04407956361232024	0.0
29	0.0	0.0	0.0	0.04407956361232024	0.0
30	0.0	0.0	0.0	0.0550994545154003	0.0
31	0.0	0.0	0.0	0.0550994545154003	0.0
32	0.0	0.0	0.0	0.0550994545154003	0.0
33	0.0	0.0	0.0	0.06060939996694033	0.0
34	0.0	0.0	0.0	0.06060939996694033	0.0
35	0.0	0.0	0.0	0.06611934541848036	0.0
36	0.0	0.0	0.0	0.06611934541848036	0.0
37	0.0	0.0	0.0	0.07713923632156042	0.0
38	0.0	0.0	0.0	0.10468896357926057	0.0
39	0.0	0.0	0.0	0.14325858174004077	0.0
40	0.0	0.0	0.0	0.19284809080390103	0.0
41	0.0	0.0	0.0	0.2589674362223814	0.0
42	0.0	0.0	0.0	0.33610667254394183	0.0
43	0.0	0.0	0.0	0.39671607251088215	0.0
44	0.0	0.0	0.0	0.4793652542839826	0.0
45	0.0	0.0	0.0	0.49589509063860265	0.0
46	0.0	0.0	0.0	0.562014436057083	0.0
47	0.0	0.0	0.0	0.6281337814755634	0.0
48	0.0	0.0	0.0	0.6832332359909636	0.0
49	0.0	0.0	0.0	0.7162929087002039	0.0
50	0.0	0.0	0.0	0.7989420904733043	0.0
51	0.0	0.0	0.0	0.8926111631494849	0.0
52	0.0	0.0	0.0	1.0413796903410657	0.0
53	0.0	0.0	0.0	1.2066780538872666	0.0
54	0.0	0.0	0.0	1.311367017466527	0.0
55	0.0	0.0	0.0	1.4160559810457876	0.0
56	0.0	0.0	0.0	1.5152349991735081	0.0
57	0.0	0.0	0.0	1.6199239627527688	0.0
58	0.0	0.0	0.0	1.7025731445258692	0.0
59	0.0	0.0	0.0	1.82379194445975	0.0
60	0.0	0.0	0.0	1.86787150807207	0.0
61	0.0	0.0	0.0	1.9284809080390104	0.0
62	0.0	0.0	0.0	1.9890903080059508	0.0
63	0.0	0.0	0.0	2.033169871618271	0.0
64	0.0	0.0	0.0	2.0937792715852113	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAA	15	0.0021895969	70.0	51
GAGGCAT	20	7.720024E-5	70.0	19
CCTACGA	20	7.720024E-5	70.0	47
GGCATAA	15	0.0021895969	70.0	21
AACTGTC	15	0.0021895969	70.0	59
TATGTTC	15	0.0021895969	70.0	51
CAACTAT	15	0.0021895969	70.0	10
TATAACA	20	7.720024E-5	70.0	29
CGTGGTA	15	0.0021895969	70.0	7
ATAACAA	15	0.0021895969	70.0	30
TCGCTCA	15	0.0021895969	70.0	69
GCTAAGC	20	7.720024E-5	70.0	8
GACAAAC	15	0.0021895969	70.0	52
TACGACA	15	0.0021895969	70.0	49
AGGCATA	20	7.720024E-5	70.0	20
ACGACAA	20	7.720024E-5	70.0	50
CGATGAG	15	0.0021895969	70.0	18
TAGCGTG	15	0.0021895969	70.0	4
AAACAGA	25	2.3285033E-4	56.000004	55
TTATAAC	25	2.3285033E-4	56.000004	28
>>END_MODULE