FastQCFastQC Report
Wed 25 May 2016
SRR1779698_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779698_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18149
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT1090.6005840542178633No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT570.3140668907377817Illumina PCR Primer Index 10 (95% over 23bp)
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA400.2203978180616012No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC350.19284809080390106Illumina PCR Primer Index 10 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC200.1101989090308006Illumina PCR Primer Index 10 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGAGA150.002189596970.08
GAAGTGT200.00684255252.56
TTGGGGA200.00684255252.560
AAGTGTG200.00684255252.57
AGTGTGA305.726549E-446.6666648
TAGAAGT450.0041984431.1111094
Produced by FastQC (version 0.11.5)