FastQCFastQC Report
Wed 25 May 2016
SRR1779697_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779697_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGAGGAGGCGGCGGACGCCGTCCTCCCCGGTGCGCACGATCAGCACCG20.10193679918450561No Hit
GTAGTAGTTACTGGTTGAACATTGTTTGTTGGTGTATATATTGTAATTGA20.10193679918450561No Hit
CCGGTGTCCCACATGGTGGACATTCGGCGTGTCACAGAACGTCCGGTTCC20.10193679918450561No Hit
CCCCCGGCTCGGTCGGGGCCGGTCTCACGCGGTCAGCGGATCACCAGGTT20.10193679918450561No Hit
GTTCTCCCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATAT20.10193679918450561No Hit
AGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAA20.10193679918450561No Hit
CTCTTGGTCTGTATTTAACCTAAATTTCTATAAGATTATTAGTATAAAAG20.10193679918450561No Hit
GGGCAGGTGGGCGCCCGCGATGCCGTCGACGGGCTCACCGTGACCCTCGC20.10193679918450561No Hit
AAAATCACCTTCCACCCTTACTACACAATCAAAGACGCCCTCGGCTTACT20.10193679918450561No Hit
CGCCGGGGCCGTGCCCCTGGCCGTCTCCGACTGCCTGAACTTCGGCTCCC20.10193679918450561No Hit
CTTATACACATCTGACGCTGCCTGTCTCTTATACACCTGTCTCTTATACA20.10193679918450561No Hit
AGTCGTAGAAGTCGGTGGGGACACCCGTCAGGCCCTCCCCCTGGTTGCCG20.10193679918450561No Hit
CGGCATCACCGTGAACCGTCTCTGCGCATCCGGCATGTCCGCGATCGGCA20.10193679918450561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACATC200.005961255652.56
ATACACA200.005961255652.54
Produced by FastQC (version 0.11.5)