##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779697_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1962
Sequences flagged as poor quality	0
Sequence length	76
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.567787971457697	33.0	23.0	34.0	12.0	34.0
2	26.433741080530073	32.0	21.0	34.0	12.0	34.0
3	25.883792048929664	31.0	21.0	34.0	11.0	34.0
4	27.672273190621816	33.0	23.0	34.0	12.0	34.0
5	27.48980632008155	33.0	23.0	34.0	11.0	34.0
6	29.084097859327215	34.0	21.0	38.0	11.0	38.0
7	30.093781855249745	36.0	25.0	38.0	12.0	38.0
8	29.113659531090725	34.0	21.0	38.0	11.0	38.0
9	30.68195718654434	37.0	26.0	38.0	12.0	38.0
10-11	30.292303771661572	36.5	25.5	38.0	11.0	38.0
12-13	29.710499490316003	35.5	23.5	38.0	11.0	38.0
14-15	29.256625891946992	34.5	21.5	38.0	11.0	38.0
16-17	29.158002038735983	35.5	23.5	38.0	11.0	38.0
18-19	29.81625891946993	35.0	23.0	38.0	11.0	38.0
20-21	28.755096839959226	32.5	22.5	37.5	11.0	38.0
22-23	29.07925586136595	34.0	23.5	38.0	11.0	38.0
24-25	29.596075433231398	35.0	22.5	38.0	11.0	38.0
26-27	28.306320081549437	33.0	21.0	38.0	11.0	38.0
28-29	30.122324159021407	36.5	23.0	38.0	11.0	38.0
30-31	30.5598878695209	37.0	24.5	38.0	11.0	38.0
32-33	30.8098878695209	37.0	26.0	38.0	11.0	38.0
34-35	30.87054026503568	37.0	26.0	38.0	11.0	38.0
36-37	29.143985728848115	34.5	22.5	38.0	11.0	38.0
38-39	27.449031600407746	31.0	21.0	37.0	11.0	38.0
40-41	28.514525993883794	34.0	21.0	37.0	11.0	38.0
42-43	29.502803261977576	35.0	22.5	38.0	11.0	38.0
44-45	30.58256880733945	37.0	24.0	38.0	11.0	38.0
46-47	29.515035677879716	35.5	23.5	38.0	11.0	38.0
48-49	28.75254841997961	34.5	17.0	38.0	11.0	38.0
50-51	26.79892966360856	31.5	11.0	37.0	11.0	38.0
52-53	29.60474006116208	35.0	23.0	37.5	11.0	38.0
54-55	28.717635066258918	35.0	21.5	38.0	11.0	38.0
56-57	29.326197757390418	35.5	22.5	38.0	11.0	38.0
58-59	30.341233435270134	36.5	24.0	38.0	11.0	38.0
60-61	30.846330275229356	37.0	25.0	38.0	11.0	38.0
62-63	30.609836901121305	37.0	24.5	38.0	11.0	38.0
64-65	30.54383282364934	37.0	24.0	38.0	11.0	38.0
66-67	28.788990825688074	34.0	21.0	38.0	11.0	38.0
68-69	28.70616717635066	34.0	21.0	38.0	11.0	38.0
70-71	27.958970438328237	34.0	20.5	37.5	11.0	38.0
72-73	27.32874617737003	31.5	20.0	37.0	11.0	38.0
74-75	28.996432212028544	35.0	22.0	37.5	11.0	38.0
76	29.266564729867483	34.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	22.0
15	36.0
16	61.0
17	68.0
18	73.0
19	80.0
20	82.0
21	72.0
22	59.0
23	40.0
24	19.0
25	40.0
26	28.0
27	43.0
28	40.0
29	59.0
30	63.0
31	67.0
32	88.0
33	121.0
34	193.0
35	220.0
36	257.0
37	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.015290519877674	18.603465851172274	10.75433231396534	35.62691131498471
2	20.846075433231395	14.72986748216106	39.70438328236494	24.71967380224261
3	19.77573904179409	14.780835881753312	37.920489296636084	27.522935779816514
4	16.51376146788991	18.90927624872579	26.24872579001019	38.328236493374106
5	16.004077471967378	23.751274209989806	38.379204892966364	21.865443425076453
6	29.051987767584098	28.287461773700308	14.984709480122325	27.675840978593275
7	27.62487257900102	23.139653414882773	15.698267074413863	33.53720693170234
8	21.55963302752294	30.886850152905197	15.443425076452598	32.11009174311927
9	21.916411824668707	14.933741080530073	16.055045871559635	47.09480122324159
10-11	25.662589194699287	19.495412844036696	24.337410805300713	30.504587155963304
12-13	23.369011213047912	15.80020387359837	28.618756371049948	32.21202854230377
14-15	26.885830784913356	19.648318042813457	21.126401630988788	32.3394495412844
16-17	28.134556574923547	20.005096839959226	21.712538226299692	30.147808358817535
18-19	23.547400611620795	18.83282364933741	25.76452599388379	31.855249745158005
20-21	27.726809378185525	18.425076452599388	22.477064220183486	31.371049949031597
22-23	27.77777777777778	18.144750254842	22.884811416921508	31.19266055045872
24-25	24.133537206931702	22.579001019367993	20.05606523955148	33.23139653414883
26-27	26.962283384301735	20.54026503567788	23.01223241590214	29.48521916411825
28-29	26.146788990825687	20.667686034658512	21.100917431192663	32.08460754332314
30-31	24.057084607543324	21.661569826707442	20.693170234454637	33.58817533129459
32-33	25.101936799184504	20.795107033639145	22.145769622833843	31.95718654434251
34-35	24.821610601427118	20.871559633027523	21.02446483180428	33.28236493374108
36-37	27.854230377166157	16.896024464831804	26.911314984709477	28.338430173292554
38-39	26.52905198776758	15.876656472986747	32.186544342507645	25.407747196738022
40-41	26.656472986748213	21.075433231396534	20.795107033639145	31.47298674821611
42-43	23.114169215086648	21.763506625891946	23.649337410805302	31.47298674821611
44-45	21.58511722731906	19.367991845056064	26.044852191641183	33.00203873598369
46-47	26.63098878695209	18.934760448521917	23.751274209989806	30.68297655453619
48-49	25.382262996941897	18.883792048929664	26.401630988786952	29.332313965341488
50-51	31.371049949031597	16.76860346585117	25.815494393476047	26.044852191641183
52-53	22.45158002038736	20.03058103975535	25.4841997961264	32.03363914373089
54-55	26.70744138634047	18.960244648318042	22.884811416921508	31.44750254841998
56-57	26.452599388379205	20.438328236493376	23.369011213047912	29.740061162079513
58-59	22.93577981651376	21.202854230377167	24.79612640163099	31.065239551478086
60-61	23.114169215086648	19.189602446483182	24.20998980632008	33.48623853211009
62-63	22.655453618756372	20.310907237512744	24.057084607543324	32.976554536187564
64-65	22.09480122324159	21.58511722731906	23.878695208970438	32.44138634046891
66-67	23.08868501529052	24.108053007135577	20.82059123343527	31.982670744138638
68-69	20.54026503567788	25.840978593272173	19.571865443425075	34.04689092762487
70-71	21.279306829765545	26.19775739041794	19.240570846075432	33.28236493374108
72-73	20.56574923547401	27.268093781855253	17.99184505606524	34.174311926605505
74-75	21.687054026503567	23.980632008154945	20.718654434250762	33.613659531090725
76	21.661569826707442	23.598369011213048	23.037716615698265	31.702344546381244
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	0.5
21	0.0
22	0.0
23	0.5
24	1.0
25	1.0
26	1.0
27	2.0
28	4.5
29	6.0
30	10.5
31	12.5
32	8.0
33	6.0
34	13.5
35	23.5
36	30.0
37	34.0
38	38.0
39	40.0
40	53.0
41	65.5
42	63.0
43	69.0
44	67.5
45	68.0
46	76.0
47	70.5
48	67.5
49	67.5
50	65.0
51	71.5
52	69.5
53	59.5
54	58.0
55	60.0
56	58.0
57	49.0
58	44.0
59	44.5
60	48.0
61	56.5
62	62.0
63	57.0
64	55.0
65	67.5
66	72.5
67	68.0
68	67.5
69	69.0
70	64.5
71	58.0
72	53.5
73	50.0
74	47.5
75	44.0
76	36.5
77	29.5
78	26.5
79	23.0
80	15.0
81	5.5
82	4.0
83	4.0
84	5.0
85	3.5
86	0.5
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	1962.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.33741080530072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.33299127757824	98.67482161060143
2	0.6670087224217547	1.325178389398573
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTGAGGAGGCGGCGGACGCCGTCCTCCCCGGTGCGCACGATCAGCACCG	2	0.10193679918450561	No Hit
GTAGTAGTTACTGGTTGAACATTGTTTGTTGGTGTATATATTGTAATTGA	2	0.10193679918450561	No Hit
CCGGTGTCCCACATGGTGGACATTCGGCGTGTCACAGAACGTCCGGTTCC	2	0.10193679918450561	No Hit
CCCCCGGCTCGGTCGGGGCCGGTCTCACGCGGTCAGCGGATCACCAGGTT	2	0.10193679918450561	No Hit
GTTCTCCCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATAT	2	0.10193679918450561	No Hit
AGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAA	2	0.10193679918450561	No Hit
CTCTTGGTCTGTATTTAACCTAAATTTCTATAAGATTATTAGTATAAAAG	2	0.10193679918450561	No Hit
GGGCAGGTGGGCGCCCGCGATGCCGTCGACGGGCTCACCGTGACCCTCGC	2	0.10193679918450561	No Hit
AAAATCACCTTCCACCCTTACTACACAATCAAAGACGCCCTCGGCTTACT	2	0.10193679918450561	No Hit
CGCCGGGGCCGTGCCCCTGGCCGTCTCCGACTGCCTGAACTTCGGCTCCC	2	0.10193679918450561	No Hit
CTTATACACATCTGACGCTGCCTGTCTCTTATACACCTGTCTCTTATACA	2	0.10193679918450561	No Hit
AGTCGTAGAAGTCGGTGGGGACACCCGTCAGGCCCTCCCCCTGGTTGCCG	2	0.10193679918450561	No Hit
CGGCATCACCGTGAACCGTCTCTGCGCATCCGGCATGTCCGCGATCGGCA	2	0.10193679918450561	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0509683995922528	0.0
21	0.0	0.0	0.0	0.1019367991845056	0.0
22	0.0	0.0	0.0	0.3058103975535168	0.0
23	0.0	0.0	0.0	0.3058103975535168	0.0
24	0.0	0.0	0.0	0.4077471967380224	0.0
25	0.0	0.0	0.0	0.4077471967380224	0.0
26	0.0	0.0	0.0	0.4077471967380224	0.0
27	0.0	0.0	0.0	0.4077471967380224	0.0
28	0.0	0.0	0.0	0.4077471967380224	0.0
29	0.0	0.0	0.0	0.45871559633027525	0.0
30	0.0	0.0	0.0	0.45871559633027525	0.0
31	0.0	0.0	0.0	0.45871559633027525	0.0
32	0.0	0.0	0.0	0.45871559633027525	0.0
33	0.0	0.0	0.0	0.45871559633027525	0.0
34	0.0	0.0	0.0	0.509683995922528	0.0
35	0.0	0.0	0.0	0.764525993883792	0.0
36	0.0	0.0	0.0	0.8154943934760448	0.0
37	0.0	0.0	0.0	0.9683995922528033	0.0
38	0.0	0.0	0.0	1.019367991845056	0.0
39	0.0	0.0	0.0	1.070336391437309	0.0
40	0.0	0.0	0.0	1.070336391437309	0.0
41	0.0	0.0	0.0	1.2232415902140672	0.0
42	0.0	0.0	0.0	1.529051987767584	0.0
43	0.0	0.0	0.0	1.5800203873598369	0.0
44	0.0	0.0	0.0	1.7329255861365953	0.0
45	0.0	0.0	0.0	1.8858307849133538	0.0
46	0.0	0.0	0.0	1.9367991845056065	0.0
47	0.0	0.0	0.0	2.038735983690112	0.0
48	0.0	0.0	0.0	2.2426095820591234	0.0
49	0.0	0.0	0.0	2.2426095820591234	0.0
50	0.0	0.0	0.0	2.3955147808358817	0.0
51	0.0	0.0	0.0	2.497451580020387	0.0
52	0.0	0.0	0.0	2.854230377166157	0.0
53	0.0	0.0	0.0	3.1600407747196737	0.0
54	0.0	0.0	0.0	3.2110091743119265	0.0
55	0.0	0.0	0.0	3.261977573904179	0.0
56	0.0	0.0	0.0	3.414882772680938	0.0
57	0.0	0.0	0.0	3.618756371049949	0.0
58	0.0	0.0	0.0	3.669724770642202	0.0
59	0.0	0.0	0.0	3.720693170234455	0.0
60	0.0	0.0	0.0	3.9755351681957185	0.0
61	0.0	0.0	0.0	4.128440366972477	0.0
62	0.0	0.0	0.0	4.230377166156982	0.0
63	0.0	0.0	0.0	4.332313965341489	0.0
64	0.0	0.0	0.0	4.5361875637105	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACATC	20	0.0059612556	52.5	6
ATACACA	20	0.0059612556	52.5	4
>>END_MODULE